{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T19:03:16Z","timestamp":1784055796626,"version":"3.55.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"W1","license":[{"start":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T00:00:00Z","timestamp":1620432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T018496\/1"],"award-info":[{"award-number":["BB\/T018496\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Saudi Arabian Government"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methods for estimating the quality of 3D models of proteins are vital tools for driving the acceptance and utility of predicted tertiary structures by the wider bioscience community. Here we describe the significant major updates to ModFOLD, which has maintained its position as a leading server for the prediction of global and local quality of 3D protein models, over the past decade (&amp;gt;20 000 unique external users). ModFOLD8 is the latest version of the server, which combines the strengths of multiple pure-single and quasi-single model methods. Improvements have been made to the web server interface and there has been successive increases in prediction accuracy, which were achieved through integration of newly developed scoring methods and advanced deep learning-based residue contact predictions. Each version of the ModFOLD server has been independently blind tested in the biennial CASP experiments, as well as being continuously evaluated via the CAMEO project. In CASP13 and CASP14, the ModFOLD7 and ModFOLD8 variants ranked among the top 10 quality estimation methods according to almost every official analysis. Prior to CASP14, ModFOLD8 was also applied for the evaluation of SARS-CoV-2 protein models as part of CASP Commons 2020 initiative. The ModFOLD8 server is freely available at: https:\/\/www.reading.ac.uk\/bioinf\/ModFOLD\/.<\/jats:p>","DOI":"10.1093\/nar\/gkab321","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T16:12:44Z","timestamp":1619107964000},"page":"W425-W430","source":"Crossref","is-referenced-by-count":74,"title":["ModFOLD8: accurate global and local quality estimates for 3D protein models"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4501-4767","authenticated-orcid":false,"given":"Liam J","family":"McGuffin","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahd M F","family":"Aldowsari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaa M A","family":"Alharbi","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Recep","family":"Adiyaman","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,5,8]]},"reference":[{"key":"2021070812053394100_B1","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1093\/bioinformatics\/btn014","article-title":"The ModFOLD server for the quality assessment of protein structural models","volume":"24","author":"McGuffin","year":"2008","journal-title":"Bioinformatics"},{"key":"2021070812053394100_B2","doi-asserted-by":"crossref","first-page":"W368","DOI":"10.1093\/nar\/gkt294","article-title":"The ModFOLD4 server for the quality assessment of 3D protein models","volume":"41","author":"McGuffin","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2021070812053394100_B3","doi-asserted-by":"crossref","first-page":"W416","DOI":"10.1093\/nar\/gkx332","article-title":"ModFOLD6: an accurate web server for the global and local quality estimation of 3D protein models","volume":"45","author":"Maghrabi","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2021070812053394100_B4","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1002\/prot.25767","article-title":"Estimation of model accuracy in CASP13","volume":"87","author":"Cheng","year":"2019","journal-title":"Proteins"},{"key":"2021070812053394100_B5","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1002\/prot.25804","article-title":"Assessment of protein model structure accuracy estimation in CASP13: challenges in the era of deep learning","volume":"87","author":"Won","year":"2019","journal-title":"Proteins"},{"key":"2021070812053394100_B6","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/prot.25360","article-title":"Accurate template-based modeling in CASP12 using the IntFOLD4-TS, ModFOLD6, and ReFOLD methods","volume":"86","author":"McGuffin","year":"2018","journal-title":"Proteins"},{"key":"2021070812053394100_B7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/978-1-0716-0708-4_4","article-title":"Estimating the quality of 3D protein models using the ModFOLD7 server","volume":"2165","author":"Maghrabi","year":"2020","journal-title":"Methods Mol. 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