{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T20:27:23Z","timestamp":1768681643589,"version":"3.49.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"W1","license":[{"start":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T00:00:00Z","timestamp":1620432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Italian Ministry of Education","award":["PRIN2017"],"award-info":[{"award-number":["PRIN2017"]}]},{"name":"European Commission H2020","award":["818290"],"award-info":[{"award-number":["818290"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Bologna ENZyme Web Server (BENZ WS) annotates four-level Enzyme Commission numbers (EC numbers) as defined by the International Union of Biochemistry and Molecular Biology (IUBMB). BENZ WS filters a target sequence with a combined system of Hidden Markov Models, modelling protein sequences annotated with the same molecular function, and Pfams, carrying along conserved protein domains. BENZ returns, when successful, for any enzyme target sequence an associated four-level EC number. Our system can annotate both monofunctional and polyfunctional enzymes, and it can be a valuable resource for sequence functional annotation.<\/jats:p>","DOI":"10.1093\/nar\/gkab328","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T21:53:21Z","timestamp":1618955601000},"page":"W60-W66","source":"Crossref","is-referenced-by-count":11,"title":["BENZ WS: the Bologna ENZyme Web Server for four-level EC number annotation"],"prefix":"10.1093","volume":"49","author":[{"given":"Davide","family":"Baldazzi","sequence":"first","affiliation":[{"name":"Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Castrense","family":"Savojardo","sequence":"additional","affiliation":[{"name":"Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0274-5669","authenticated-orcid":false,"given":"Pier Luigi","family":"Martelli","sequence":"additional","affiliation":[{"name":"Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Casadio","sequence":"additional","affiliation":[{"name":"Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy"},{"name":"Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IBIOM), Italian National Research Council (CNR), Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,5,8]]},"reference":[{"key":"2021070812053801900_B1","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkaa1100","article-title":"UniProt: the universal protein knowledgebase in 2021","volume":"49","author":"The UniProt Consortium","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2021070812053801900_B2","doi-asserted-by":"crossref","first-page":"D437","DOI":"10.1093\/nar\/gkaa1038","article-title":"RCSB Protein Data Bank: powerful new tools for exploring 3D structures of biological macromolecules for basic and applied research and education in fundamental biology, biomedicine, biotechnology, bioengineering and energy sciences","volume":"49","author":"Burley","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2021070812053801900_B3","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s13059-019-1835-8","article-title":"The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens","volume":"20","author":"Zhou","year":"2019","journal-title":"Genome biology"},{"key":"2021070812053801900_B4","doi-asserted-by":"crossref","first-page":"D325","DOI":"10.1093\/nar\/gkaa1113","article-title":"The Gene Ontology resource: enriching a GOld mine","volume":"49","author":"Gene Ontology Consortium","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2021070812053801900_B5","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/protein\/gzx008","article-title":"An update on the Enzyme Portal: an integrative approach for exploring enzyme knowledge","volume":"30","author":"Pundir","year":"2017","journal-title":"Protein Eng. 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