{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:20:01Z","timestamp":1792441201314,"version":"4.5.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T00:00:00Z","timestamp":1633305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003845","name":"Indian Institute of Technology Madras","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003845","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology, Government of India","award":["CRG\/2020\/000314"],"award-info":[{"award-number":["CRG\/2020\/000314"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Protein\u2013nucleic acid interactions are involved in various biological processes such as gene expression, replication, transcription, translation and packaging. The binding affinities of protein\u2013DNA and protein\u2013RNA complexes are important for elucidating the mechanism of protein\u2013nucleic acid recognition. Although experimental data on binding affinity are reported abundantly in the literature, no well-curated database is currently available for protein\u2013nucleic acid binding affinity. We have developed a database, ProNAB, which contains more than 20 000 experimental data for the binding affinities of protein\u2013DNA and protein\u2013RNA complexes. Each entry provides comprehensive information on sequence and structural features of a protein, nucleic acid and its complex, experimental conditions, thermodynamic parameters such as dissociation constant (Kd), binding free energy (\u0394G) and change in binding free energy upon mutation (\u0394\u0394G), and literature information. ProNAB is cross-linked with GenBank, UniProt, PDB, ProThermDB, PROSITE, DisProt and Pubmed. It provides a user-friendly web interface with options for search, display, sorting, visualization, download and upload the data. ProNAB is freely available at https:\/\/web.iitm.ac.in\/bioinfo2\/pronab\/ and it has potential applications such as understanding the factors influencing the affinity, development of prediction tools, binding affinity change upon mutation and design complexes with the desired affinity.<\/jats:p>","DOI":"10.1093\/nar\/gkab848","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T15:10:08Z","timestamp":1631286608000},"page":"D1528-D1534","source":"Crossref","is-referenced-by-count":63,"title":["ProNAB: database for binding affinities of protein\u2013nucleic acid complexes and their mutants"],"prefix":"10.1093","volume":"50","author":[{"given":"Kannan","family":"Harini","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambuj","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arulsamy","family":"Kulandaisamy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1776-4096","authenticated-orcid":false,"given":"M Michael","family":"Gromiha","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"2022010507401870200_B1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/bs.ctdb.2015.11.018","article-title":"The soft touch: low-affinity transcription factor binding sites in development and evolution","volume":"117","author":"Crocker","year":"2016","journal-title":"Curr. 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Oncol."},{"key":"2022010507401870200_B5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1128\/JCM.02118-12","article-title":"Neutralizing DNA aptamers against swine influenza H3N2 viruses","volume":"51","author":"Wongphatcharachai","year":"2013","journal-title":"J. Clin. Microbial."},{"key":"2022010507401870200_B6","doi-asserted-by":"crossref","first-page":"11796","DOI":"10.1038\/s41598-017-10070-1","article-title":"A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry","volume":"7","author":"Xu","year":"2017","journal-title":"Sci. Rep."},{"key":"2022010507401870200_B7","doi-asserted-by":"crossref","first-page":"10361","DOI":"10.1002\/ange.202100345","article-title":"Neutralizing aptamers block S\/RBD-ACE2 interactions and prevent host cell infection","volume":"133","author":"Liu","year":"2021","journal-title":"Angew. Chem. Weinheim. Bergstr. 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