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Stochastic V(D)J recombination generates massive TCR\/BCR repertoire diversity. Single-cell immune profiling with transcriptome analysis allows the high-throughput study of individual TCR\/BCR clonotypes and functions under both normal and pathological settings. However, a comprehensive database linking these data is not yet readily available. Here, we present the human Antigen Receptor database (huARdb), a large-scale human single-cell immune profiling database that contains 444 794 high confidence T or B cells (hcT\/B cells) with full-length TCR\/BCR sequence and transcriptomes from 215 datasets. All datasets were processed in a uniform workflow, including sequence alignment, cell subtype prediction, unsupervised cell clustering, and clonotype definition. We also developed a multi-functional and user-friendly web interface that provides interactive visualization modules for biologists to analyze the transcriptome and TCR\/BCR features at the single-cell level. HuARdb is freely available at https:\/\/huarc.net\/database with functions for data querying, browsing, downloading, and depositing. In conclusion, huARdb is a comprehensive and multi-perspective atlas for human antigen receptors.<\/jats:p>","DOI":"10.1093\/nar\/gkab857","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T11:19:43Z","timestamp":1631618383000},"page":"D1244-D1254","source":"Crossref","is-referenced-by-count":23,"title":["huARdb: human Antigen Receptor database for interactive clonotype-transcriptome analysis at the single-cell level"],"prefix":"10.1093","volume":"50","author":[{"given":"Lize","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Immunology and Department of Rheumatology at Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"},{"name":"Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3491-2433","authenticated-orcid":false,"given":"Ziwei","family":"Xue","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Siqian","family":"Jin","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Jinchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Yixin","family":"Guo","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Yadan","family":"Bai","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Xuexiao","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Department of Rheumatology at Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"}]},{"given":"Chaochen","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"Lie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Immunology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"}]},{"given":"Zuozhu","family":"Liu","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Illinois at Urbana-Champaign Institute (ZJU-UIUC Institute), International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"given":"James Q","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0171-4115","authenticated-orcid":false,"given":"Linrong","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Department of Rheumatology at Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China"},{"name":"Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China"},{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"},{"name":"Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5963-2908","authenticated-orcid":false,"given":"Wanlu","family":"Liu","sequence":"additional","affiliation":[{"name":"Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China"},{"name":"Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, International Campus, Zhejiang University, Haining, Zhejiang 314400, China"},{"name":"Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China"},{"name":"Department of Orthopedic Surgery of the Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China"},{"name":"Alibaba-Zhejiang University Joint Research Center of Future Digital Healthcare, Zhejiang University, Hangzhou, Zhejiang 310058, China"}]}],"member":"286","published-online":{"date-parts":[[2021,10,4]]},"reference":[{"key":"2022010507331977500_B1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1055\/s-2006-939525","article-title":"Immunologic and oncologic implications of laparoscopic surgery: what is the latest","volume":"19","author":"Lee","year":"2006","journal-title":"Clin. 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