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RNA-sequencing data from human tissues has shown that this process can be inaccurate, resulting in the presence of novel introns detected at low frequency across samples and within an individual. To enable the full spectrum of intron use to be explored, we have developed IntroVerse, which offers an extensive catalogue on the splicing of 332,571 annotated introns and a linked set of 4,679,474 novel junctions covering 32,669 different genes. This dataset has been generated through the analysis of 17,510 human control RNA samples from 54 tissues provided by the Genotype-Tissue Expression Consortium. IntroVerse has two unique features: (i) it provides a complete catalogue of novel junctions and (ii) each novel junction has been assigned to a specific annotated intron. This unique, hierarchical structure offers multiple uses, including the identification of novel transcripts from known genes and their tissue-specific usage, and the assessment of background splicing noise for introns thought to be mis-spliced in disease states. IntroVerse provides a user-friendly web interface and is freely available at https:\/\/rytenlab.com\/browser\/app\/introverse.<\/jats:p>","DOI":"10.1093\/nar\/gkac1056","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T18:34:54Z","timestamp":1668796494000},"page":"D167-D178","source":"Crossref","is-referenced-by-count":16,"title":["IntroVerse: a comprehensive database of introns across human tissues"],"prefix":"10.1093","volume":"51","author":[{"given":"Sonia","family":"Garc\u00eda-Ruiz","sequence":"first","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0541-7537","authenticated-orcid":false,"given":"Emil K","family":"Gustavsson","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2382-8460","authenticated-orcid":false,"given":"David","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina H","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongbo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aine","family":"Fairbrother-Browne","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"},{"name":"Department of Medical and Molecular Genetics, School of Basic and Medical Biosciences, King's College London , London , WC2R\u00a02LS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luisa","family":"Gil-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"},{"name":"Department of Information and Communications Engineering Faculty of Informatics, Espinardo Campus, University of Murcia , Murcia , 30100, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A","family":"Botia","sequence":"additional","affiliation":[{"name":"Department of Information and Communications Engineering Faculty of Informatics, Espinardo Campus, University of Murcia , Murcia , 30100, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Collado-Torres","sequence":"additional","affiliation":[{"name":"Lieber Institute for Brain Development , Baltimore , MD\u00a021205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9520-6957","authenticated-orcid":false,"given":"Mina","family":"Ryten","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Medicine Research & Teaching, UCL GOS Institute of Child Health , London , WC1N\u00a01EH, UK"},{"name":"Department of Neurodegenerative Disease, Queen Square Institute of Neurology , UCL, London \u00a0WC1N 3BG, UK"},{"name":"NIHR Great Ormond Street Hospital Biomedical Research Centre, University College London , London , WC1N\u00a01EH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,18]]},"reference":[{"key":"2023010804261250100_B1","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.1073\/pnas.74.8.3171","article-title":"Spliced segments at the 5\u2019 terminus of adenovirus 2 late mRNA","volume":"74","author":"Berget","year":"1977","journal-title":"Proc. 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