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Manual curation of all the available human cell line perturbed RNA-seq datasets enabled us to develop a comprehensive human perturbation database (GPSAdb, https:\/\/www.gpsadb.com\/). The current version of GPSAdb collected 3048 RNA-seq datasets associated with 1458 genes, which were knocked out\/down by siRNA, shRNA, CRISPR\/Cas9, or CRISPRi. The database provides full exploration of these datasets and generated 6096 new perturbed gene sets (up and down separately). GPSAdb integrated the gene sets and developed an online tool, genetic perturbation similarity analysis (GPSA), to identify candidate causal perturbations from differential gene expression data. In summary, GPSAdb is a powerful platform that aims to assist life science researchers to easily access and analyze public perturbed data and explore differential gene expression data in depth.<\/jats:p>","DOI":"10.1093\/nar\/gkac1066","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T11:12:05Z","timestamp":1669201925000},"page":"D964-D968","source":"Crossref","is-referenced-by-count":39,"title":["GPSAdb: a comprehensive web resource for interactive exploration of genetic perturbation RNA-seq datasets"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9286-7132","authenticated-orcid":false,"given":"Shipeng","family":"Guo","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and 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