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There are 90 papers reporting on new databases and 82 updates from resources previously published in the Issue. Six more papers are updates from databases most recently published elsewhere. Major nucleic acid databases reporting updates include Genbank, ENA, ChIPBase, JASPAR, mirDIP and the Issue's first Breakthrough Article, NACDDB for Circular Dichroism data. Updates from BMRB and RCSB cover experimental protein structural data while AlphaFold 2 computational structure predictions feature widely. STRING and REBASE are stand-out updates in the signalling and enzymes section. Immunology-related databases include CEDAR, the second Breakthrough Article, for cancer epitopes and receptors alongside returning IPD-IMGT\/HLA and the new PGG.MHC. Genomics-related resources include Ensembl, GWAS Central and UCSC Genome Browser. Major returning databases for drugs and their targets include Open Targets, DrugCentral, CTD and Pubchem. The EMPIAR image archive appears in the Issue for the first time. The entire database Issue is freely available online on the Nucleic Acids Research website (https:\/\/academic.oup.com\/nar). The NAR online Molecular Biology Database Collection has been updated, revisiting 463 entries, adding 92 new resources and eliminating 96 discontinued URLs so bringing the current total to 1764 databases. It is available at http:\/\/www.oxfordjournals.org\/nar\/database\/c\/.<\/jats:p>","DOI":"10.1093\/nar\/gkac1186","type":"journal-article","created":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T04:39:56Z","timestamp":1673152796000},"page":"D1-D8","source":"Crossref","is-referenced-by-count":29,"title":["The 2023 <i>Nucleic Acids Research<\/i> Database Issue and the online molecular biology database collection"],"prefix":"10.1093","volume":"51","author":[{"given":"Daniel J","family":"Rigden","sequence":"first","affiliation":[{"name":"Institute of Systems, Molecular and Integrative Biology, University of Liverpool , Crown Street, Liverpool L69 7ZB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xos\u00e9 M","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"IQVIA Ltd., The Point, 37 North Wharf Road , London W2 1AF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"key":"2023011102261768000_B1","article-title":"EMBL\u2019s European Bioinformatics Institute (EMBL-EBI) in 2022","author":"Thakur","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2023011102261768000_B2","article-title":"Database resources of the National Center for Biotechnology Information in 2023","author":"Sayers","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B3","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkab951","article-title":"Database resources of the National Genomics Data Center, China National Center for Bioinformation in 2023","author":"CNCB-NGDC Members and Partners","year":"2022","journal-title":"Nucleic\u00a0Acids Res."},{"key":"2023011102261768000_B4","article-title":"NACDDB: nucleic acid circular dichroism Database","author":"Cappannini","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2023011102261768000_B5","article-title":"G4Atlas: a comprehensive transcriptome-wide G-quadruplex database","author":"Yu","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B6","article-title":"QUADRatlas: the RNA G-quadruplex and RG4-binding proteins database","author":"Bourdon","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B7","article-title":"GAIA: g-quadruplexes in alive creature database","author":"Vannutelli","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B8","article-title":"tModBase: deciphering the landscape of tRNA modifications and their dynamic changes from epitranscriptome data","author":"Lei","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B9","article-title":"RM2Target: a comprehensive database for targets of writers, erasers and readers of RNA modifications","author":"Bao","year":"2022","journal-title":"Nucleic\u00a0Acids\u00a0Res."},{"key":"2023011102261768000_B10","doi-asserted-by":"crossref","first-page":"bbaa055","DOI":"10.1093\/bib\/bbaa055","article-title":"M6A2Target: a comprehensive database for targets of m6A writers, erasers and readers","volume":"22","author":"Deng","year":"2021","journal-title":"Brief. 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