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Visualization of big omics data is essential to answer a wide range of biological problems. As a concise but comprehensive strategy, a heatmap can analyze and visualize high-dimensional and heterogeneous biomolecular expression data in an attractive artwork. In 2014, we developed a stand-alone software package, Heat map Illustrator (HemI 1.0), which implemented three clustering methods and seven distance metrics for heatmap illustration. Here, we significantly improved 1.0 and released the online service of HemI 2.0, in which 7 clustering methods and 22 types of distance metrics were implemented. In HemI 2.0, the clustering results and publication-quality heatmaps can be exported directly. For an in-depth analysis of the data, we further added an option of enrichment analysis for 12 model organisms, with 15 types of functional annotations. The enrichment results can be visualized in five idioms, including bubble chart, bar graph, coxcomb chart, pie chart and word cloud. We anticipate that HemI 2.0 can be a helpful web server for visualization of biomolecular expression data, as well as the additional enrichment analysis. HemI 2.0 is freely available for all users at: https:\/\/hemi.biocuckoo.org\/.<\/jats:p>","DOI":"10.1093\/nar\/gkac480","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T13:50:34Z","timestamp":1654609834000},"page":"W405-W411","source":"Crossref","is-referenced-by-count":44,"title":["HemI 2.0: an online service for heatmap illustration"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1410-7891","authenticated-orcid":false,"given":"Wanshan","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan , Hubei 430022 , China"},{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Yuxiang","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1232-8866","authenticated-orcid":false,"given":"Letian","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Cheng","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Yujie","family":"Gou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Shanshan","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Xinhe","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Sicheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Di","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"given":"Chenwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9403-6869","authenticated-orcid":false,"given":"Yu","family":"Xue","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Center for Artificial Intelligence Biology, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , Hubei 430074 , China"},{"name":"Nanjing University Institute of Artificial Intelligence Biomedicine , Nanjing \u00a0210031, China"}]}],"member":"286","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"2022070423564893900_B1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0958-1669(99)00060-9","article-title":"Visualization of data","volume":"11","author":"Meyer","year":"2000","journal-title":"Curr. 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