{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T19:03:19Z","timestamp":1780945399203,"version":"3.54.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF1200900"],"award-info":[{"award-number":["2021YFF1200900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0507504"],"award-info":[{"award-number":["2018YFA0507504"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070666"],"award-info":[{"award-number":["32070666"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Phase separation (PS) proteins form droplets to regulate myriad membraneless organelles (MLOs) and cellular pathways such as transcription, signaling transduction and protein degeneration. PS droplets are usually liquid-like and can convert to hydrogel\/solid-like under certain conditions. The PS behavior of proteins is regulated by co-PS partners and mutations, modifications, oligomerizations, repeat regions and alternative splicing of the proteins. With growing interest in PS condensates and associated proteins, we established PhaSepDB 1.0, which provided experimentally verified PS proteins and MLO-related proteins. The past few\u00a0years witnessed a surge in PS-related research works; thus, we kept updating PhaSepDB. The current PhaSepDB contains 1419 PS entries, 770 low-throughput MLO-related entries and 7303 high-throughput MLO-related entries. We provided more detailed annotations of PS proteins, including PS verification experiments, regions used in experiments, phase diagrams of different experimental conditions, droplet states, co-PS partners and PS regulatory information. We believe that researchers can go further in studying PS proteins with the updated PhaSepDB (http:\/\/db.phasep.pro\/).<\/jats:p>","DOI":"10.1093\/nar\/gkac783","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T08:45:52Z","timestamp":1663663552000},"page":"D460-D465","source":"Crossref","is-referenced-by-count":85,"title":["PhaSepDB in 2022: annotating phase separation-related proteins with droplet states, co-phase separation partners and other experimental information"],"prefix":"10.1093","volume":"51","author":[{"given":"Chao","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotai","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Peking University First Hospital , Beijing \u00a0100034,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5038-1185","authenticated-orcid":false,"given":"Kaiqiang","family":"You","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4266-0317","authenticated-orcid":false,"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191,\u00a0 China"},{"name":"Key Laboratory for Neuroscience, Ministry of Education\/National Health Commission of China, Peking University , Beijing \u00a0100191,\u00a0 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"2023010804333474000_B1","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s11427-020-1702-x","article-title":"Liquid\u2013liquid phase separation in biology: mechanisms, physiological functions and human diseases","volume":"63","author":"Zhang","year":"2020","journal-title":"Sci. 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