{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T09:19:32Z","timestamp":1771924772859,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170656"],"award-info":[{"award-number":["32170656"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["Z211100002121039"],"award-info":[{"award-number":["Z211100002121039"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinical Medicine Plus X - Young Scholars Project, Peking University","award":["PKU2022LCXQ043"],"award-info":[{"award-number":["PKU2022LCXQ043"]}]},{"DOI":"10.13039\/501100009399","name":"Peking University Third Hospital","doi-asserted-by":"publisher","award":["BYSYYZD2021001"],"award-info":[{"award-number":["BYSYYZD2021001"]}],"id":[{"id":"10.13039\/501100009399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Project of Collaborative Innovation Center of GDAAS","award":["XT202218"],"award-info":[{"award-number":["XT202218"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CRISPR-Cas base editing (BE) system is a powerful tool to expand the scope and efficiency of genome editing with single-nucleotide resolution. The editing efficiency, product purity, and off-target effect differ among various BE systems. Herein, we developed CRISPRbase (http:\/\/crisprbase.maolab.org), by integrating 1 252 935 records of base editing outcomes in more than 50 cell types from 17 species. CRISPRbase helps to evaluate the putative editing precision of different BE systems by integrating multiple annotations, functional predictions and a blasting system for single-guide RNA sequences. We systematically assessed the editing window, editing efficiency and product purity of various BE systems. Intensive efforts were focused on increasing the editing efficiency and product purity of base editors since the byproduct could be detrimental in certain applications. Remarkably, more than half of cancer-related off-target mutations were non-synonymous and extremely damaging to protein functions in most common tumor types. Luckily, most of these cancer-related mutations were passenger mutations (4840\/5703, 84.87%) rather than cancer driver mutations (863\/5703, 15.13%), indicating a weak effect of off-target mutations on carcinogenesis. In summary, CRISPRbase is a powerful and convenient tool to study the outcomes of different base editors and help researchers choose appropriate BE designs for functional studies.<\/jats:p>","DOI":"10.1093\/nar\/gkac967","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T16:38:15Z","timestamp":1668011895000},"page":"D1249-D1256","source":"Crossref","is-referenced-by-count":7,"title":["Annotation and evaluation of base editing outcomes in multiple cell types using CRISPRbase"],"prefix":"10.1093","volume":"51","author":[{"given":"Jibiao","family":"Fan","sequence":"first","affiliation":[{"name":"Institute of Medical Innovation and Research, Peking University Third Hospital , Beijing \u00a0100191, China"},{"name":"College of Animal Science and Technology, Yangzhou University , Yangzhou , Jiangsu Province\u00a0 225009 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leisheng","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Medical Innovation and Research, Peking University Third Hospital , Beijing \u00a0100191, China"},{"name":"Cancer Center, Peking University Third Hospital , Beijing \u00a0100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou \u00a0510640, China"},{"name":"Guangdong Key Laboratory of New Technology in Rice Breeding , Guangzhou \u00a0510640, China"},{"name":"Guangdong Rice Engineering Laboratory , Guangzhou \u00a0510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Medical Innovation and Research, Peking University Third Hospital , Beijing \u00a0100191, 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Degui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Guangdong Academy of Agricultural Sciences , Guangzhou \u00a0510640, China"},{"name":"Guangdong Key Laboratory of New Technology in Rice Breeding , Guangzhou \u00a0510640, China"},{"name":"Guangdong Rice Engineering Laboratory , Guangzhou \u00a0510640, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Marine Protozoan Biodiversity & Evolution, Marine College, Shandong University , Weihai \u00a0264209, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University Health Science Center , Beijing \u00a0100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixiang","family":"Xue","sequence":"additional","affiliation":[{"name":"Institute of Medical Innovation and Research, Peking University Third Hospital , Beijing \u00a0100191, China"},{"name":"Cancer Center, Peking University Third Hospital , Beijing \u00a0100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lichao","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing \u00a0100021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0852-4266","authenticated-orcid":false,"given":"Fengbiao","family":"Mao","sequence":"additional","affiliation":[{"name":"Institute of Medical Innovation and Research, Peking University Third 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