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In its latest version (v4.0, http:\/\/www.microrna.gr\/miRPathv4), DIANA-miRPath breaks new ground by introducing the capacity to tailor its target-based miRNA functional analysis engine to specific biological and\/or experimental contexts. Via a redesigned modular interface with rich interaction, annotation and parameterization options, users can now perform enrichment analysis on Gene Ontology (GO) terms, KEGG and REACTOME pathways, sets from Molecular Signatures Database (MSigDB) and PFAM. Included miRNA interaction sets are derived from state-of-the-art resources of experimentally supported (DIANA-TarBase v8.0, miRTarBase and microCLIP cell-type-specific interactions) or from in silico miRNA\u2013target interactions (updated DIANA-microT-CDS and TargetScan predictions). Bulk and single-cell expression datasets from The Cancer Genome Atlas (TCGA), the Genotype-Tissue Expression project (GTEx) and adult\/fetal single-cell atlases are integrated and can be used to assess the expression of enriched term components across a wide range of states. A discrete module enabling enrichment analyses using CRISPR knock-out screen datasets enables the detection of selected miRNAs with potentially crucial roles within conditions under study. Notably, the option to upload custom interaction, term, expression\u00a0and screen sets further expands the versatility of miRPath webserver.<\/jats:p>","DOI":"10.1093\/nar\/gkad431","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T14:24:24Z","timestamp":1685543064000},"page":"W154-W159","source":"Crossref","is-referenced-by-count":143,"title":["DIANA-miRPath v4.0: expanding target-based miRNA functional analysis in cell-type and tissue contexts"],"prefix":"10.1093","volume":"51","author":[{"given":"Spyros","family":"Tastsoglou","sequence":"first","affiliation":[{"name":"DIANA-Lab, Department of Computer Science and Biomedical Informatics, Univ. of Thessaly , Lamia \u00a035131, Greece"},{"name":"Hellenic Pasteur Institute , Athens \u00a011521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgos","family":"Skoufos","sequence":"additional","affiliation":[{"name":"DIANA-Lab, Department of Computer Science and Biomedical 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