{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:54:01Z","timestamp":1788893641824,"version":"build-2803163510"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T00:00:00Z","timestamp":1508889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,4]]},"DOI":"10.1093\/nar\/gkx993","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T15:28:02Z","timestamp":1507822082000},"page":"D776-D780","source":"Crossref","is-referenced-by-count":82,"title":["PICKLES: the database of pooled in-vitro CRISPR knockout library essentiality screens"],"prefix":"10.1093","volume":"46","author":[{"given":"Walter F","family":"Lenoir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tassica L","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-3341","authenticated-orcid":false,"given":"Traver","family":"Hart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,10,25]]},"reference":[{"key":"key\n\t\t\t\t20180103190733_B1","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.cell.2006.01.040","article-title":"A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen","volume":"124","year":"2006","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1038\/nature03353","article-title":"Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans","volume":"434","year":"2005","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180103190733_B3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/nmeth1006-777","article-title":"Minimizing the risk of reporting false positives in large-scale RNAi screens","volume":"3","year":"2006","journal-title":"Nat. Methods"},{"key":"key\n\t\t\t\t20180103190733_B4","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrg1836","article-title":"High-throughput RNAi screening in cultured cells: a user's guide","volume":"7","year":"2006","journal-title":"Nat. Rev. Genet."},{"key":"key\n\t\t\t\t20180103190733_B5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.tips.2007.02.009","article-title":"Off-target effects associated with long dsRNAs in Drosophila RNAi screens","volume":"28","year":"2007","journal-title":"Trends Pharmacol. Sci."},{"key":"key\n\t\t\t\t20180103190733_B6","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1126\/science.1225787","article-title":"Molecular biology. Use and abuse of RNAi to study mammalian gene function","volume":"337","year":"2012","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B7","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1126\/science.1231143","article-title":"Multiplex genome engineering using CRISPR\/Cas systems","volume":"339","year":"2013","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B8","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","year":"2013","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B9","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","year":"2014","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B10","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","year":"2014","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B11","doi-asserted-by":"crossref","first-page":"aah7111","DOI":"10.1126\/science.aah7111","article-title":"CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells","volume":"355","year":"2017","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B12","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1038\/nbt.3715","article-title":"Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library","volume":"34","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"key\n\t\t\t\t20180103190733_B13","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.cell.2014.09.029","article-title":"Genome-scale CRISPR-mediated control of gene repression and activation","volume":"159","year":"2014","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B14","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/nature14136","article-title":"Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex","volume":"517","year":"2015","journal-title":"Nature"},{"key":"key\n\t\t\t\t20180103190733_B15","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.cell.2013.02.022","article-title":"Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression","volume":"152","year":"2013","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B16","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/nbt.3199","article-title":"Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers","volume":"33","year":"2015","journal-title":"Nat. Biotechnol"},{"key":"key\n\t\t\t\t20180103190733_B17","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nmeth.4286","article-title":"Genetic interaction mapping in mammalian cells using CRISPR interference","volume":"14","year":"2017","journal-title":"Nat. Methods"},{"key":"key\n\t\t\t\t20180103190733_B18","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1016\/j.celrep.2016.09.079","article-title":"A CRISPR dropout screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia","volume":"17","year":"2016","journal-title":"Cell Rep."},{"key":"key\n\t\t\t\t20180103190733_B19","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.aac7041","article-title":"Identification and characterization of essential genes in the human genome","volume":"350","year":"2015","journal-title":"Science"},{"key":"key\n\t\t\t\t20180103190733_B20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/nm.4219","article-title":"Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors","volume":"23","year":"2017","journal-title":"Nat. Med."},{"key":"key\n\t\t\t\t20180103190733_B21","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.cell.2017.01.013","article-title":"Gene essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras","volume":"168","year":"2017","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B22","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1158\/2159-8290.CD-16-0154","article-title":"Genomic copy number dictates a gene-independent cell response to CRISPR\/Cas9 targeting","volume":"6","year":"2016","journal-title":"Cancer Discov."},{"key":"key\n\t\t\t\t20180103190733_B23","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.cell.2017.07.005","article-title":"Project DRIVE: a compendium of cancer dependencies and synthetic lethal relationships uncovered by large-scale, deep RNAi screening","volume":"170","year":"2017","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B24","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.cell.2017.06.010","article-title":"Defining a cancer dependency map","volume":"170","year":"2017","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B25","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12859-016-1015-8","article-title":"BAGEL: a computational framework for identifying essential genes from pooled library screens","volume":"17","year":"2016","journal-title":"BMC Bioinformatics"},{"key":"key\n\t\t\t\t20180103190733_B26","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1158\/2159-8290.CD-11-0224","article-title":"Essential gene profiles in breast, pancreatic, and ovarian cancer cells","volume":"2","year":"2012","journal-title":"Cancer Discov."},{"key":"key\n\t\t\t\t20180103190733_B27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.cell.2015.11.062","article-title":"Functional genomic landscape of human breast cancer drivers, vulnerabilities, and resistance","volume":"164","year":"2016","journal-title":"Cell"},{"key":"key\n\t\t\t\t20180103190733_B28","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1016\/j.celrep.2017.02.028","article-title":"Interrogation of functional cell-surface markers identifies CD151 dependency in high-grade serous ovarian cancer","volume":"18","year":"2017","journal-title":"Cell Rep."},{"key":"key\n\t\t\t\t20180103190733_B29","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1534\/g3.117.041277","article-title":"Evaluation and design of genome-wide CRISPR\/SpCas9 knockout screens","volume":"7","year":"2017","journal-title":"G3 (Bethesda)"},{"key":"key\n\t\t\t\t20180103190733_B30","first-page":"96","article-title":"International system for human gene nomenclature (1979) ISGN (1979)","volume":"15","year":"1980","journal-title":"Birth Defects Orig. Artic. Ser."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/46\/D1\/D776\/23162942\/gkx993.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,4]],"date-time":"2019-10-04T11:43:34Z","timestamp":1570189414000},"score":1,"resource":{"primary":{"URL":"http:\/\/academic.oup.com\/nar\/article\/46\/D1\/D776\/4564803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,25]]},"references-count":30,"journal-issue":{"issue":"D1","published-online":{"date-parts":[[2017,10,25]]},"published-print":{"date-parts":[[2018,1,4]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkx993","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,1,4]]},"published":{"date-parts":[[2017,10,25]]}}}