{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:26:11Z","timestamp":1780471571956,"version":"3.54.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"D1","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science","award":["RTI 2018-095584-B-C42"],"award-info":[{"award-number":["RTI 2018-095584-B-C42"]}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/R019002\/1"],"award-info":[{"award-number":["EP\/R019002\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The Standard European Vector Architecture 3.0 database (SEVA-DB 3.0, http:\/\/seva.cnb.csic.es) is the update of the platform launched in 2013 both as a web-based resource and as a material repository of formatted genetic tools (mostly plasmids) for analysis, construction and deployment of complex bacterial phenotypes. The period between the first version of SEVA-DB and the present time has witnessed several technical, computational and conceptual advances in genetic\/genomic engineering of prokaryotes that have enabled upgrading of the utilities of the updated database. Novelties include not only a more user-friendly web interface and many more plasmid vectors, but also new links of the plasmids to advanced bioinformatic tools. These provide an intuitive visualization of the constructs at stake and a range of virtual manipulations of DNA segments that were not possible before. Finally, the list of canonical SEVA plasmids is available in machine-readable SBOL (Synthetic Biology Open Language) format. This ensures interoperability with other platforms and affords simulations of their behaviour under different in vivo conditions. We argue that the SEVA-DB will remain a useful resource for extending Synthetic Biology approaches towards non-standard bacterial species as well as genetically programming new prokaryotic chassis for a suite of fundamental and biotechnological endeavours.<\/jats:p>","DOI":"10.1093\/nar\/gkz1024","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T15:10:50Z","timestamp":1571411450000},"page":"D1164-D1170","source":"Crossref","is-referenced-by-count":99,"title":["SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts"],"prefix":"10.1093","volume":"48","author":[{"given":"Esteban","family":"Mart\u00ednez-Garc\u00eda","sequence":"first","affiliation":[{"name":"Systems Biology Program, Centro Nacional de Biotecnolog\u00eda CSIC, Campus de la Universidad Aut\u00f3noma de Madrid, 28049 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Go\u00f1i-Moreno","sequence":"first","affiliation":[{"name":"School of Computing, Newcastle University NE4 5TG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Bartley","sequence":"first","affiliation":[{"name":"Raytheon BBN Technologies, Arlington, VA 22209, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"McLaughlin","sequence":"first","affiliation":[{"name":"School of Computing, Newcastle University NE4 5TG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"S\u00e1nchez-Sampedro","sequence":"first","affiliation":[{"name":"Scienseed SL, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor","family":"Pascual\u00a0del\u00a0Pozo","sequence":"first","affiliation":[{"name":"Scienseed SL, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Prieto\u00a0Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Scienseed SL, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ada Serena","family":"Marletta","sequence":"first","affiliation":[{"name":"Doulix-Explora Srl, Via G. 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