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We upgraded our original coding potential calculator, CNCI (Coding-Non-Coding Index), to CNIT (Coding-Non-Coding Identifying Tool), which provides faster and more accurate evaluation of the coding ability of RNA transcripts. CNIT runs\u00a0\u223c200 times faster than CNCI and exhibits more accuracy compared with CNCI (0.98 versus 0.94 for human, 0.95 versus 0.93 for mouse, 0.93 versus 0.92 for zebrafish, 0.93 versus 0.92 for fruit fly, 0.92 versus 0.88 for worm, and 0.98 versus 0.85 for Arabidopsis transcripts). Moreover, the AUC values of 11 animal species and 27 plant species showed that CNIT was capable of obtaining relatively accurate identification results for almost all eukaryotic transcripts. In addition, a mobile-friendly web server is now freely available at http:\/\/cnit.noncode.org\/CNIT.<\/jats:p>","DOI":"10.1093\/nar\/gkz400","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T15:13:13Z","timestamp":1556809993000},"page":"W516-W522","source":"Crossref","is-referenced-by-count":132,"title":["CNIT: a fast and accurate web tool for identifying protein-coding and long non-coding transcripts based on intrinsic sequence composition"],"prefix":"10.1093","volume":"47","author":[{"given":"Jin-Cheng","family":"Guo","sequence":"first","affiliation":[{"name":"Beijing University of Chinese Medicine, Chaoyang District, Beijing 100029, China"},{"name":"Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou 515041, China"},{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Shuang-Sang","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}]},{"given":"Yang","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing University of Chinese Medicine, Chaoyang District, Beijing 100029, China"},{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Jian-Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion, Peking University People's Hospital, Beijing 100000, China"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou 515041, China"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"The College of Life Sciences, Northwest University, Xi\u2019an 710069, China"}]},{"given":"Bo","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Jia-Rui","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing University of Chinese Medicine, Chaoyang District, Beijing 100029, China"}]},{"given":"En-Min","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou 515041, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1618-4292","authenticated-orcid":false,"given":"Li-Yan","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biology in High Cancer Incidence Coastal Chaoshan Area of Guangdong Higher Education Institutes, Shantou University Medical College, Shantou 515041, China"}]},{"given":"Liang","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Intelligent Information Processing, Advanced Computer Research Center, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing University of Chinese Medicine, Chaoyang District, Beijing 100029, China"}]}],"member":"286","published-online":{"date-parts":[[2019,5,31]]},"reference":[{"key":"2019062808230816600_B1","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1038\/35103511","article-title":"Non-coding RNA genes and the modern RNA world","volume":"2","author":"Eddy","year":"2001","journal-title":"Nat. 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