{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:58:07Z","timestamp":1761101887627},"reference-count":33,"publisher":"China Science Publishing & Media Ltd.","issue":"4","content-domain":{"domain":["engine.scichina.com"],"crossmark-restriction":false},"short-container-title":["ABBS"],"published-print":{"date-parts":[[2010,4,1]]},"DOI":"10.1093\/abbs\/gmq014","type":"journal-article","created":{"date-parts":[[2010,3,3]],"date-time":"2010-03-03T01:25:51Z","timestamp":1267579551000},"page":"266-273","update-policy":"http:\/\/dx.doi.org\/10.1360\/scp-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Tandem affinity purification and identification of the human TSC1 protein complex"],"prefix":"10.3724","volume":"42","author":[{"given":"Longhua","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wantao","family":"Ying","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanzhi","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuchu","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2026","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"null","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1357-4310(98)01245-3","volume":"4","author":"Young J","year":"1998","unstructured":"Young J, Povey S. The genetic basis of tuberous sclerosis. Mol Med Today, 1998, 4: 313-319.","journal-title":"Mol Med Today"},{"key":"null","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1093\/hmg\/7.6.1053","volume":"7","author":"van Slegtenhorst M","year":"1998","unstructured":"van Slegtenhorst M, Nellist M, Nagelkerken B, Cheadle J, Snell R, van den Ouweland A, Reuser A. Interaction between hamartin and tuberin, the TSC1 and TSC2 gene products. Hum Mol Genet, 1998, 7: 1053-1057.","journal-title":"Hum Mol Genet"},{"key":"null","doi-asserted-by":"crossref","first-page":"35647","DOI":"10.1074\/jbc.274.50.35647","volume":"274","author":"Nellist M","year":"1999","unstructured":"Nellist M, van Slegtenhorst MA, Goedbloed M, van den Ouweland AM, Halley DJ, van der Sluijs P. Characterization of the cytosolic tuberin-hamartin complex. Tuberin is a cytosolic chaperone for hamartin. J Biol Chem, 1999, 274: 35647-35652.","journal-title":"J Biol Chem"},{"key":"null","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S0898-6568(03)00040-8","volume":"15","author":"Krymskaya VP","year":"2003","unstructured":"Krymskaya VP. Tumour suppressors hamartin and tuberin: intracellular signalling. Cell Signal, 2003, 15: 729-739.","journal-title":"Cell Signal"},{"key":"null","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S1383-5742(01)00058-8","volume":"488","author":"Hengstschlager M","year":"2001","unstructured":"Hengstschlager M, Rodman DM, Miloloza A, Hengstschlager-Ottnad E, Rosner M, Kubista M. Tuberous sclerosis gene products in proliferation control. Mutat Res, 2001, 488: 233-239.","journal-title":"Mutat Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1016\/j.bbrc.2005.05.175","volume":"333","author":"Nellist M","year":"2005","unstructured":"Nellist M, Burgers PC, van den Ouweland AM, Halley DJ, Luider TM. Phosphorylation and binding partner analysis of the TSC1-TSC2 complex. Biochem Biophys Res Commun, 2005, 333: 818-826.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1073\/pnas.0409143102","volume":"102","author":"Ballif BA","year":"2005","unstructured":"Ballif BA, Roux PP, Gerber SA, MacKeigan JP, Blenis J, Gygi SP. Quantitative phosphorylation profiling of the ERK\/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proc Natl Acad Sci USA, 2005, 102: 667-672.","journal-title":"Proc Natl Acad Sci USA"},{"key":"null","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0092-8674(00)80922-8","volume":"92","author":"Alberts B","year":"1998","unstructured":"Alberts B. The cell as a collection of protein machines: preparing the next generation of molecular biologists. Cell, 1998, 92: 291-294.","journal-title":"Cell"},{"key":"null","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S1367-5931(02)00007-8","volume":"7","author":"Gavin AC","year":"2003","unstructured":"Gavin AC, Superti-Furga G. Protein complexes and proteome organization from yeast to man. Curr Opin Chem Biol, 2003, 7: 21-27.","journal-title":"Curr Opin Chem Biol"},{"key":"null","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1006\/bbrc.1998.8853","volume":"247","author":"Orimoto K","year":"1998","unstructured":"Orimoto K, Tsuchiya H, Sakurai J, Nishizawa M, Hino O. Identification of cDNAs induced by the tumor suppressor Tsc2 gene using a conditional expression system in Tsc2 mutant (Eker) rat renal carcinoma cells. Biochem Biophys Res Commun, 1998, 247: 728-733.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.mrfmmm.2003.12.023","volume":"549","author":"Sen B","year":"2004","unstructured":"Sen B, Wolf DC, Hester SD. The transcriptional profile of the kidney in Tsc2 heterozygous mutant Long Evans (Eker) rats compared to wild-type. Mutat Res, 2004, 549: 213-224.","journal-title":"Mutat Res"},{"key":"null","first-page":"1411","volume":"27","author":"Rosner M","year":"2005","unstructured":"Rosner M, Freilinger A, Lubec G, Hengstschlager M. The tuberous sclerosis genes, TSC1 and TSC2, trigger different gene expression responses. Int J Oncol, 2005, 27: 1411-1424.","journal-title":"Int J Oncol"},{"key":"null","doi-asserted-by":"crossref","first-page":"4786","DOI":"10.1038\/sj.onc.1206776","volume":"22","author":"Rosner M","year":"2003","unstructured":"Rosner M, Hofer K, Kubista M, Hengstschlager M. Cell size regulation by the human TSC tumor suppressor proteins depends on PI3K and FKBP38. Oncogene, 2003, 22: 4786-4798.","journal-title":"Oncogene"},{"key":"null","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/S0006-291X(03)01238-5","volume":"307","author":"Hengstschlager M","year":"2003","unstructured":"Hengstschlager M, Rosner M, Fountoulakis M, Lubec G. Regulation of PCNA and CAF-1 expression by the two tuberous sclerosis gene products. Biochem Biophys Res Commun, 2003, 307: 737-742.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.bbrc.2003.10.170","volume":"312","author":"Hengstschlager M","year":"2003","unstructured":"Hengstschlager M, Rosner M, Fountoulakis M, Lubec G. Tuberous sclerosis genes regulate cellular 14-3-3 protein levels. Biochem Biophys Res Commun, 2003, 312: 676-683.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.canlet.2004.01.021","volume":"210","author":"Hengstschlager M","year":"2004","unstructured":"Hengstschlager M, Rosner M, Fountoulakis M, Oh JE, Lubec G. Protein levels of alpha1-tubulin, protein disulfide isomerase, tropomyosins and vimentin are regulated by the tuberous sclerosis gene products. Cancer Lett, 2004, 210: 219-226.","journal-title":"Cancer Lett"},{"key":"null","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1038\/13732","volume":"17","author":"Rigaut G","year":"1999","unstructured":"Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B. A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol, 1999, 17: 1030-1032.","journal-title":"Nat Biotechnol"},{"key":"null","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1006\/meth.2001.1183","volume":"24","author":"Puig O","year":"2001","unstructured":"Puig O, Caspary F, Rigaut G, Rutz B, Bouveret E, Bragado-Nilsson E, Wilm M. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods, 2001, 24: 218-229.","journal-title":"Methods"},{"key":"null","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1113\/jphysiol.2004.080440","volume":"563","author":"Gingras AC","year":"2005","unstructured":"Gingras AC, Aebersold R, Raught B. Advances in protein complex analysis using mass spectrometry. J Physiol, 2005, 563: 11-21.","journal-title":"J Physiol"},{"key":"null","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1126\/science.1074276","volume":"297","author":"Gao G","year":"2002","unstructured":"Gao G, Guo X, Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. Science, 2002, 297: 1703-1706.","journal-title":"Science"},{"key":"null","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1021\/pr049761h","volume":"4","author":"Jin WH","year":"2005","unstructured":"Jin WH, Dai J, Li SJ, Xia QC, Zou HF, Zeng R. Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification. J Proteome Res, 2005, 4: 613-619.","journal-title":"J Proteome Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0003-9861(02)00300-4","volume":"404","author":"Yamamoto Y","year":"2002","unstructured":"Yamamoto Y, Jones KA, Mak BC, Muehlenbachs A, Yeung RS. Multicompartmental distribution of the tuberous sclerosis gene products, hamartin and tuberin. Arch Biochem Biophys, 2002, 404: 210-217.","journal-title":"Arch Biochem Biophys"},{"key":"null","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1212\/01.WNL.0000140702.08902.D7","volume":"63","author":"Jansen FE","year":"2004","unstructured":"Jansen FE, Notenboom RGE, Nellist M, Goedbloed MA, Halley DJ, de Graan PNE, van Nieuwenhuizen O. Differential localization of hamartin and tuberin and increased S6 phosphorylation in a tuber. Neurology, 2004, 63: 1293-1295.","journal-title":"Neurology"},{"key":"null","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/labinvest.3780143","volume":"80","author":"Fukuda T","year":"2000","unstructured":"Fukuda T, Kobayashi T, Momose S, Yasui H, Hino O. Distribution of Tsc1 protein detected by immunohistochemistry in various normal rat tissues and the renal carcinomas of Eker rat: detection of limited colocalization with Tsc1 and Tsc2 gene products in vivo. Lab Invest, 2000, 80: 1347-1359.","journal-title":"Lab Invest"},{"key":"null","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S0962-8924(00)01898-5","volume":"11","author":"Burkhard P","year":"2001","unstructured":"Burkhard P, Stetefeld J, Strelkov SV. Coiled coils: a highly versatile protein folding motif. Trends Cell Biol, 2001, 11: 82-88.","journal-title":"Trends Cell Biol"},{"key":"null","doi-asserted-by":"crossref","first-page":"10745","DOI":"10.1074\/jbc.M709211200","volume":"283","author":"Shirai A","year":"2008","unstructured":"Shirai A, Matsuyama A, Yashiroda Y, Hashimoto A, Kawamura Y, Arai R, Komatsu Y. Global analysis of gel mobility of proteins and its use in target identification. J Biol Chem, 2008, 283: 10745-10752.","journal-title":"J Biol Chem"},{"key":"null","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1477-5956-3-6","volume":"3","author":"Ahmad QR","year":"2005","unstructured":"Ahmad QR, Nguyen DH, Wingerd MA, Church GM, Steffen MA. Molecular weight assessment of proteins in total proteome profiles using 1D-PAGE and LC\/MS\/MS. Proteome Sci, 2005, 3:","journal-title":"Proteome Sci"},{"key":"null","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.mrrev.2008.01.001","volume":"658","author":"Rosner M","year":"2008","unstructured":"Rosner M, Hanneder M, Siegel N, Valli A, Hengstschlager M. The tuberous sclerosis gene products hamartin and tuberin are multifunctional proteins with a wide spectrum of interacting partners. Mutat Res, 2008, 658: 234-246.","journal-title":"Mutat Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.dnarep.2004.03.015","volume":"3","author":"Lieber MR","year":"2004","unstructured":"Lieber MR, Ma Y, Pannicke U, Schwarz K. The mechanism of vertebrate nonhomologous DNA end joining and its role in V(D)J recombination. DNA Repair (Amst), 2004, 3: 817-826.","journal-title":"DNA Repair (Amst)"},{"key":"null","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.0105-2896.2004.00162.x","volume":"200","author":"Meek K","year":"2004","unstructured":"Meek K, Gupta S, Ramsden DA, Lees-Miller SP. The DNA-dependent protein kinase: the director at the end. Immunol Rev, 2004, 200: 132-141.","journal-title":"Immunol Rev"},{"key":"null","doi-asserted-by":"crossref","first-page":"5205","DOI":"10.1074\/jbc.M412758200","volume":"280","author":"Bladen CL","year":"2005","unstructured":"Bladen CL, Udayakumar D, Takeda Y, Dynan WS. Identification of the polypyrimidine tract binding protein-associated splicing factor. p54(nrb) complex as a candidate DNA double-strand break rejoining factor. J Biol Chem, 2005, 280: 5205-5210.","journal-title":"J Biol Chem"},{"key":"null","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1038\/sj.onc.1201743","volume":"16","author":"Soucek T","year":"1998","unstructured":"Soucek T, Holzl G, Bernaschek G, Hengstschlager M. A role of the tuberous sclerosis gene-2 product during neuronal differentiation. Oncogene, 1998, 16: 2197-2204.","journal-title":"Oncogene"},{"key":"null","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1038\/nn1566","volume":"8","author":"Tavazoie SF","year":"2005","unstructured":"Tavazoie SF, Alvarez VA, Ridenour DA, Kwiatkowski DJ, Sabatini BL. Regulation of neuronal morphology and function by the tumor suppressors Tsc1 and Tsc2. Nat Neurosci, 2005, 8: 1727-1734.","journal-title":"Nat Neurosci"}],"container-title":["Acta Biochimica et Biophysica Sinica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/engine.scichina.com\/doi\/pdf\/F36B675396344FFE9A4CD1E0FBE3DF18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/engine.scichina.com\/doi\/10.1093\/abbs\/gmq014","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/engine.scichina.com\/doi\/pdf\/F36B675396344FFE9A4CD1E0FBE3DF18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,24]],"date-time":"2024-03-24T15:27:14Z","timestamp":1711294034000},"score":1,"resource":{"primary":{"URL":"http:\/\/engine.scichina.com\/doi\/10.1093\/abbs\/gmq014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,1]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2010,4,1]]},"published-print":{"date-parts":[[2010,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/abbs\/gmq014","relation":{},"ISSN":["1672-9145"],"issn-type":[{"value":"1672-9145","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4,1]]}}}