{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T08:28:15Z","timestamp":1770539295992,"version":"3.49.0"},"reference-count":47,"publisher":"China Science Publishing & Media Ltd.","issue":"10","content-domain":{"domain":["engine.scichina.com"],"crossmark-restriction":false},"short-container-title":["ABBS"],"published-print":{"date-parts":[[2012,10,1]]},"DOI":"10.1093\/abbs\/gms070","type":"journal-article","created":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T22:29:50Z","timestamp":1348784990000},"page":"856-865","update-policy":"https:\/\/doi.org\/10.1360\/scp-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Lentiviral-mediated gene transfer into human adipose-derived stem cells: role of NELL1 versus BMP2 in osteogenesis and adipogenesis &amp;lt;italic&amp;gt;in vitro&amp;lt;\/italic&amp;gt;"],"prefix":"10.3724","volume":"44","author":[{"given":"Yajing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiyun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huixia","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongsheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LingLing","family":"E","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongchen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2026","published-online":{"date-parts":[[2012,10,1]]},"reference":[{"key":"null","doi-asserted-by":"crossref","first-page":"4279","DOI":"10.1091\/mbc.e02-02-0105","volume":"13","author":"Zuk PA","year":"2002","unstructured":"Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13: 4279-4295.","journal-title":"Mol Biol Cell"},{"key":"null","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.bbrc.2007.02.165","volume":"356","author":"Li H","year":"2007","unstructured":"Li H, Dai K, Tang T, Zhang X, Yan M, Lou J. Bone regeneration by implantation of adipose-derived stromal cells expressing BMP-2. Biochem Biophys Res Commun, 2007, 356: 836-842.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1242\/dev.122.10.2977","volume":"122","author":"Zhang H","year":"1996","unstructured":"Zhang H, Bradley A. Mice deficient for BMP2 are nonviable and have defects in amnion\/chorion and cardiac development. Development, 1996, 122: 2977-2986.","journal-title":"Development"},{"key":"null","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1006\/bbrc.1997.7497","volume":"239","author":"Kusumoto K","year":"1997","unstructured":"Kusumoto K, Bessho K, Fujimura K, Akioka J, Ogawa Y, Iizuka T. Comparison of ectopic osteoinduction in vivo by recombinant human BMP-2 and recombinant Xenopus BMP-4\/7 heterodimer. Biochem Biophys Res Commun, 1997, 239: 575-579.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.spinee.2009.10.016","volume":"10","author":"Deutsch H","year":"2010","unstructured":"Deutsch H. High-dose bone morphogenetic protein-induced ectopic abdomen bone growth. Spine J, 2010, 10: e1-4.","journal-title":"Spine J"},{"key":"null","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1359\/jbmr.1999.14.1.80","volume":"14","author":"Ting K","year":"1999","unstructured":"Ting K, Vastardis H, Mulliken JB, Soo C, Tieu A, Do H, Kwong E. Human NELL-1 expressed in unilateral coronal synostosis. J Bone Miner Res, 1999, 14: 80-89.","journal-title":"J Bone Miner Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1172\/JCI15375","volume":"110","author":"Zhang X","year":"2002","unstructured":"Zhang X, Kuroda S, Carpenter D, Nishimura I, Soo C, Moats R, Iida K. Craniosynostosis in transgenic mice overexpressing Nell-1. J Clin Invest, 2002, 110: 861-870.","journal-title":"J Clin Invest"},{"key":"null","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1093\/hmg\/ddl053","volume":"15","author":"Desai J","year":"2006","unstructured":"Desai J, Shannon ME, Johnson MD, Ruff DW, Hughes LA, Kerley MK, Carpenter DA. Nell1-deficient mice have reduced expression of extracellular matrix proteins causing cranial and vertebral defects. Hum Mol Genet, 2006, 15: 1329-1341.","journal-title":"Hum Mol Genet"},{"key":"null","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1177\/0022034510376401","volume":"89","author":"Zhang X","year":"2010","unstructured":"Zhang X, Zara J, Siu RK, Ting K, Soo C. The role of NELL-1, a growth factor associated with craniosynostosis, in promoting bone regeneration. J Dent Res, 2010, 89: 865-878.","journal-title":"J Dent Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"903","DOI":"10.2353\/ajpath.2006.051210","volume":"169","author":"Aghaloo T","year":"2006","unstructured":"Aghaloo T, Cowan CM, Chou YF, Zhang X, Lee H, Miao S, Hong N. Nell-1-induced bone regeneration in calvarial defects. Am J Pathol, 2006, 169: 903-915.","journal-title":"Am J Pathol"},{"key":"null","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1038\/sj.mt.6300270","volume":"15","author":"Aghaloo T","year":"2007","unstructured":"Aghaloo T, Jiang X, Soo C, Zhang Z, Zhang X, Hu J, Pan H. A study of the role of nell-1 gene modified goat bone marrow stromal cells in promoting new bone formation. Mol Ther, 2007, 15: 1872-1880.","journal-title":"Mol Ther"},{"key":"null","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.spinee.2006.04.020","volume":"7","author":"Lu SS","year":"2007","unstructured":"Lu SS, Zhang X, Soo C, Hsu T, Napoli A, Aghaloo T, Wu BM. The osteoinductive properties of Nell-1 in a rat spinal fusion model. Spine J, 2007, 7: 50-60.","journal-title":"Spine J"},{"key":"null","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.bone.2010.10.166","volume":"48","author":"Xue J","year":"2011","unstructured":"Xue J, Peng J, Yuan M, Wang A, Zhang L, Liu S, Fan M. NELL1 promotes high-quality bone regeneration in rat femoral distraction osteogenesis model. Bone, 2011, 48: 485-495.","journal-title":"Bone"},{"key":"null","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1359\/jbmr.061012","volume":"22","author":"Truong T","year":"2007","unstructured":"Truong T, Zhang X, Pathmanathan D, Soo C, Ting K. Craniosynostosis-associated gene nell-1 is regulated by runx2. J Bone Miner Res, 2007, 22: 7-18.","journal-title":"J Bone Miner Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/jbmr.267","volume":"26","author":"Zhang X","year":"2011","unstructured":"Zhang X, Ting K, Bessette CM, Culiat CT, Sung SJ, Lee H, Chen F. Nell-1, a key functional mediator of Runx2, partially rescues calvarial defects in Runx2(+\/-) mice. J Bone Miner Res, 2011, 26: 777-791.","journal-title":"J Bone Miner Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1359\/jbmr.070312","volume":"22","author":"Cowan CM","year":"2007","unstructured":"Cowan CM, Jiang X, Hsu T, Soo C, Zhang B, Wang JZ, Kuroda S. Synergistic effects of Nell-1 and BMP-2 on the osteogenic differentiation of myoblasts. J Bone Miner Res, 2007, 22: 918-930.","journal-title":"J Bone Miner Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcb.20411","volume":"95","author":"Zhao M","year":"2005","unstructured":"Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7. J Cell Biochem, 2005, 95: 1-16.","journal-title":"J Cell Biochem"},{"key":"null","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1080\/17453670510041709","volume":"76","author":"Xu XL","year":"2005","unstructured":"Xu XL, Tang T, Dai K, Zhu Z, Guo XE, Yu C, Lou J. Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats. Acta Orthop, 2005, 76: 637-646.","journal-title":"Acta Orthop"},{"key":"null","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1038\/sj.gt.3302785","volume":"13","author":"Egermann M","year":"2006","unstructured":"Egermann M, Lill CA, Griesbeck K, Evans CH, Robbins PD, Schneider E, Baltzer AW. Effect of BMP-2 gene transfer on bone healing in sheep. Gene Ther, 2006, 13: 1290-1299.","journal-title":"Gene Ther"},{"key":"null","doi-asserted-by":"crossref","first-page":"11382","DOI":"10.1073\/pnas.93.21.11382","volume":"93","author":"Naldini L","year":"1996","unstructured":"Naldini L, Bl\u00f6mer U, Gage FH, Trono D, Verma IM. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A, 1996, 93: 11382-11388.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"null","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1126\/science.272.5259.263","volume":"272","author":"Naldini L","year":"1996","unstructured":"Naldini L, Bl\u00f6mer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science, 1996, 272: 263-267.","journal-title":"Science"},{"key":"null","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1002\/jor.20229","volume":"24","author":"Feeley BT","year":"2006","unstructured":"Feeley BT, Conduah AH, Sugiyama O, Krenek L, Chen IS, Lieberman JR. In vivo molecular imaging of adenoviral versus lentiviral gene therapy in two bone formation models. J Orthop Res, 2006, 24: 1709-1721.","journal-title":"J Orthop Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1097\/BRS.0b013e3181761003","volume":"33","author":"Miyazaki M","year":"2008","unstructured":"Miyazaki M, Sugiyama O, Zou J, Yoon SH, Wei F, Morishita Y, Sintuu C. Comparison of lentiviral and adenoviral gene therapy for spinal fusion in rats. Spine (Phila Pa 1976), 2008, 33: 1410-1417.","journal-title":"Spine (Phila Pa 1976)"},{"key":"null","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/j.bone.2007.12.216","volume":"42","author":"Virk MS","year":"2008","unstructured":"Virk MS, Conduah A, Park SH, Liu N, Sugiyama O, Cuomo A, Kang C. Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model. Bone, 2008, 42: 921-931.","journal-title":"Bone"},{"key":"null","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1172\/JCI21311","volume":"113","author":"Pei L","year":"2004","unstructured":"Pei L, Tontonoz P. Fat's loss is bone's gain. J Clin Invest, 2004, 113: 805-806.","journal-title":"J Clin Invest"},{"key":"null","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/jcb.20777","volume":"98","author":"Gimble JM","year":"2006","unstructured":"Gimble JM, Zvonic S, Floyd ZE, Kassem M, Nuttall ME. Playing with bone and fat. J Cell Biochem, 2006, 98: 251-266.","journal-title":"J Cell Biochem"},{"key":"null","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1517\/14728220902915310","volume":"13","author":"Takada I","year":"2009","unstructured":"Takada I, Kouzmenko AP, Kato S. Molecular switching of osteoblastogenesis versus adipogenesis: implications for targeted therapies. Expert Opin Ther Targets, 2009, 13: 593-603.","journal-title":"Expert Opin Ther Targets"},{"key":"null","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1091\/mbc.e02-06-0356","volume":"14","author":"Hata K","year":"2003","unstructured":"Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T. Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis. Mol Biol Cell, 2003, 14: 545-555.","journal-title":"Mol Biol Cell"},{"key":"null","doi-asserted-by":"crossref","first-page":"34015","DOI":"10.1074\/jbc.M403621200","volume":"279","author":"Ichida F","year":"2004","unstructured":"Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H. Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation. J Biol Chem, 2004, 279: 34015-34022.","journal-title":"J Biol Chem"},{"key":"null","doi-asserted-by":"crossref","first-page":"12670","DOI":"10.1073\/pnas.0906266106","volume":"106","author":"Huang H","year":"2009","unstructured":"Huang H, Song TJ, Li X, Hu L, He Q, Liu M, Lane MD. BMP signaling pathway is required for commitment of C3H10T1\/2 pluripotent stem cells to the adipocyte lineage. Proc Natl Acad Sci U S A, 2009, 106: 12670-12675.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"null","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.bbrc.2011.06.111","volume":"411","author":"James AW","year":"2011","unstructured":"James AW, Pan A, Chiang M, Zara JN, Zhang X, Ting K. A new function of Nell-1 protein in repressing adipogenic differentiation. Biochem Biophys Res Commun, 2011, 411: 126-131.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1006\/bbrc.1996.1573","volume":"227","author":"Zhang G","year":"1996","unstructured":"Zhang G, Gurtu V, Kain SR. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem Biophys Res Commun, 1996, 227: 707-711.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s00441-009-0883-x","volume":"338","author":"Arrigoni E","year":"2009","unstructured":"Arrigoni E, Lopa S, de Girolamo L, Stanco D, Brini AT. Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models. Cell Tissue Res, 2009, 338: 401-411.","journal-title":"Cell Tissue Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1097\/PRS.0b013e3181da8858","volume":"126","author":"Levi B","year":"2010","unstructured":"Levi B, James AW, Wan DC, Glotzbach JP, Commons GW, Longaker MT. Regulation of human adipose-derived stromal cell osteogenic differentiation by insulin-like growth factor-1 and platelet-derived growth factor-alpha. Plast Reconstr Surg, 2010, 126: 41-52.","journal-title":"Plast Reconstr Surg"},{"key":"null","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/S0092-8674(00)80257-3","volume":"89","author":"Ducy P","year":"1997","unstructured":"Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. Osf2\/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell, 1997, 89: 747-754.","journal-title":"Cell"},{"key":"null","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0092-8674(01)00622-5","volume":"108","author":"Nakashima K","year":"2002","unstructured":"Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, de Crombrugghe B. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell, 2002, 108: 17-29.","journal-title":"Cell"},{"key":"null","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1177\/154405910508401204","volume":"84","author":"Franceschi RT","year":"2005","unstructured":"Franceschi RT. Biological Approaches to Bone Regeneration by Gene Therapy. J Dent Res, 2005, 84: 1093-1103.","journal-title":"J Dent Res"},{"key":"null","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1006\/mthe.2002.0542","volume":"5","author":"Vigna E","year":"2002","unstructured":"Vigna E, Cavalieri S, Ailles L, Geuna M, Loew R, Bujard H, Naldini L. Robust and efficient regulation of transgene expression in vivo by improved tetracycline-dependent lentiviral vectors. Mol Ther, 2002, 5: 252-261.","journal-title":"Mol Ther"},{"key":"null","doi-asserted-by":"crossref","first-page":"8463","DOI":"10.1128\/JVI.72.11.8463-8471.1998","volume":"72","author":"Dull T","year":"1998","unstructured":"Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. A third-generation lentivirus vector with a conditional packaging system. J Virol, 1998, 72: 8463-8471.","journal-title":"J Virol"},{"key":"null","first-page":"107","volume":"261","author":"Zufferey R","year":"2002","unstructured":"Zufferey R. Production of lentiviral vectors. Curr Top Microbiol Immunol, 2002, 261: 107-121.","journal-title":"Curr Top Microbiol Immunol"},{"key":"null","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/S8756-3282(02)00826-8","volume":"31","author":"Nishimura R","year":"2002","unstructured":"Nishimura R, Hata K, Harris SE, Ikeda F, Yoneda T. Core-binding factor alpha 1 (Cbfa1) induces osteoblastic differentiation of C2C12 cells without interactions with Smad1 and Smad5. Bone, 2002, 31: 303-312.","journal-title":"Bone"},{"key":"null","doi-asserted-by":"crossref","first-page":"29119","DOI":"10.1074\/jbc.M801774200","volume":"283","author":"Matsubara T","year":"2008","unstructured":"Matsubara T, Kida K, Yamaguchi A, Hata K, Ichida F, Meguro H, Aburatani H. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J Biol Chem, 2008, 283: 29119-29125.","journal-title":"J Biol Chem"},{"key":"null","doi-asserted-by":"crossref","first-page":"e24638","DOI":"10.1371\/journal.pone.0024638","volume":"6","author":"Chen F","year":"2011","unstructured":"Chen F, Zhang X, Sun S, Zara JN, Zou X, Chiu R, Culiat CT. NELL-1, an osteoinductive factor, is a direct transcriptional target of Osterix. PLoS One, 2011, 6:","journal-title":"PLoS One"},{"key":"null","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1006\/bbrc.1998.8200","volume":"244","author":"Takase M","year":"1998","unstructured":"Takase M, Imamura T, Sampath TK, Takeda K, Ichijo H, Miyazono K, Kawabata M. Induction of Smad6 mRNA by bone morphogenetic proteins. Biochem Biophys Res Commun, 1998, 244: 26-29.","journal-title":"Biochem Biophys Res Commun"},{"key":"null","doi-asserted-by":"crossref","first-page":"507","DOI":"10.2337\/diabetes.47.4.507","volume":"47","author":"Spiegelman BM","year":"1998","unstructured":"Spiegelman BM. PPAR-gamma: adipogenic regulator and thiazolidinedione receptor. Diabetes, 1998, 47: 507-514.","journal-title":"Diabetes"},{"key":"null","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev.cellbio.16.1.145","volume":"16","author":"Rosen ED","year":"2000","unstructured":"Rosen ED, Spiegelman BM. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol, 2000, 16: 145-171.","journal-title":"Annu Rev Cell Dev Biol"},{"key":"null","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s00223-009-9250-x","volume":"85","author":"Lin L","year":"2009","unstructured":"Lin L, Shen Q, Wei X, Hou Y, Xue T, Fu X, Duan X. Comparison of osteogenic potentials of BMP4 transduced stem cells from autologous bone marrow and fat tissue in a rabbit model of calvarial defects. Calcif Tissue Int, 2009, 85: 55-65.","journal-title":"Calcif Tissue Int"}],"container-title":["Acta Biochimica et Biophysica Sinica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/engine.scichina.com\/doi\/pdf\/67CD0A5C137C40F9928F68DBBA6259CD","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/engine.scichina.com\/doi\/10.1093\/abbs\/gms070","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/engine.scichina.com\/doi\/pdf\/67CD0A5C137C40F9928F68DBBA6259CD","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T21:24:00Z","timestamp":1714425840000},"score":1,"resource":{"primary":{"URL":"http:\/\/engine.scichina.com\/doi\/10.1093\/abbs\/gms070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,1]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2012,10,1]]},"published-print":{"date-parts":[[2012,10,1]]}},"URL":"https:\/\/doi.org\/10.1093\/abbs\/gms070","relation":{},"ISSN":["1672-9145"],"issn-type":[{"value":"1672-9145","type":"print"}],"subject":[],"published":{"date-parts":[[2012,10,1]]}}}