{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:19:54Z","timestamp":1740136794019,"version":"3.37.3"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>C-C chemokine receptor type 4 (CCR4) proteins are expressed on the neoplastic cells of adult T-cell leukemia\/lymphoma (ATLL). As the mutation status of CCR4 gene is reported to correlate with significant clinical information such as prognosis and response to mogamulizumab, we aimed to establish a screening method that is suitable for clinical laboratory tests.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In 34 patients with ATLL, CCR4 mutation analysis, high-resolution melting (HRM) analysis, fragment analysis, and direct sequencing were performed using both genomic DNA and complementary DNA (cDNA). Furthermore, 38 cases of asymptomatic carriers of human T-cell leukemia virus type 1 (HTLV-1) were screened for CCR4 mutation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Mutation analysis by direct sequencing of 34 ATLL clinical samples detected CCR4 mutation in four genomic DNA samples and seven cDNA samples, and two novel mutations were identified. All CCR4 mutations detected by direct sequencing were positive for HRM analysis and\/or fragment analysis. CCR4 mutation was not detected in the asymptomatic carriers of HTLV-1.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CCR4 mutation screening by a combination of HRM and fragment analysis using cDNA is a simple and practical method, and it will contribute to better decision making for a therapeutic strategy, providing a rapid CCR4 mutational status to clinicians.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqaa037","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T12:14:43Z","timestamp":1582719283000},"page":"236-241","source":"Crossref","is-referenced-by-count":1,"title":["cDNA-Based Mutation Screening Using a Combination of High-Resolution Melting Curve and Fragment Analysis Facilitates Efficient CCR4 Mutation Analysis in Adult T-Cell Leukemia\/Lymphoma"],"prefix":"10.1093","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2688-8522","authenticated-orcid":false,"given":"Shumpei","family":"Mizuta","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Hyogo Prefectural Amagasaki General Medical Center, Hyogo, Japan"},{"name":"Department of Hematology, Hyogo Prefectural Amagasaki General Medical Center, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Yamane","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Hyogo Prefectural Amagasaki General Medical Center, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saya","family":"Mononobe","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Hyogo Prefectural Amagasaki General Medical Center, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Komai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Hyogo Prefectural Amagasaki General Medical Center, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Koba","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahito","family":"Kawata","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan"},{"name":"Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Ukyo","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tamekane","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsumasa","family":"Watanabe","sequence":"first","affiliation":[{"name":"Laboratory of Hematology, Division of Medical Biophysics, Kobe University Graduate School of Health Sciences, Hyogo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,5,5]]},"reference":[{"key":"2020070702341808300_CIT0001","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1182\/blood.V50.3.481.481","article-title":"Adult T-cell leukemia: clinical and hematologic features of 16 cases","volume":"50","author":"Uchiyama","year":"1977","journal-title":"Blood."},{"key":"2020070702341808300_CIT0002","doi-asserted-by":"crossref","first-page":"7415","DOI":"10.1073\/pnas.77.12.7415","article-title":"Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma","volume":"77","author":"Poiesz","year":"1980","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020070702341808300_CIT0003","doi-asserted-by":"crossref","first-page":"6476","DOI":"10.1073\/pnas.78.10.6476","article-title":"Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera","volume":"78","author":"Hinuma","year":"1981","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020070702341808300_CIT0004","doi-asserted-by":"crossref","first-page":"2534","DOI":"10.1073\/pnas.81.8.2534","article-title":"Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease","volume":"81","author":"Yoshida","year":"1984","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020070702341808300_CIT0005","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/294770a0","article-title":"Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells","volume":"294","author":"Miyoshi","year":"1981","journal-title":"Nature."},{"key":"2020070702341808300_CIT0006","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s12185-018-02588-5","article-title":"JSH practical guidelines for hematological malignancies, 2018: II. lymphoma-8. Adult T-cell leukemia-lymphoma","volume":"109","author":"Tsukasaki","year":"2019","journal-title":"Int J Hematol."},{"key":"2020070702341808300_CIT0007","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1093\/ajcp\/91.2.152","article-title":"Immunohistochemical analysis of peripheral T-cell lymphoma in Japanese patients","volume":"91","author":"Doi","year":"1989","journal-title":"Am J Clin Pathol."},{"key":"2020070702341808300_CIT0008","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1182\/blood-2004-03-1222","article-title":"Overexpression of a cell adhesion molecule, TSLC1, as a possible molecular marker for acute-type adult T-cell leukemia","volume":"105","author":"Sasaki","year":"2005","journal-title":"Blood."},{"key":"2020070702341808300_CIT0009","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1182\/blood.V99.5.1505","article-title":"Frequent expression of CCR4 in adult T-cell leukemia and human T-cell leukemia virus type 1-transformed T cells","volume":"99","author":"Yoshie","year":"2002","journal-title":"Blood."},{"key":"2020070702341808300_CIT0010","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/j.1365-2141.1991.tb08051.x","article-title":"Diagnostic criteria and classification of clinical subtypes of adult T-cell leukaemia-lymphoma: a report from the Lymphoma Study Group (1984-87)","volume":"79","author":"Shimoyama","year":"1991","journal-title":"Br J Haematol."},{"key":"2020070702341808300_CIT0011","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1200\/JCO.2011.38.2101","article-title":"Prognostic index for acute- and lymphoma-type adult T-cell leukemia\/lymphoma","volume":"30","author":"Katsuya","year":"2012","journal-title":"J Clin Oncol."},{"key":"2020070702341808300_CIT0012","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1182\/blood-2017-01-757542","article-title":"Prognostic index for chronic- and smoldering-type adult T-cell leukemia-lymphoma","volume":"130","author":"Katsuya","year":"2017","journal-title":"Blood."},{"key":"2020070702341808300_CIT0013","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1158\/1078-0432.CCR-09-2697","article-title":"Defucosylated humanized anti-CCR4 monoclonal antibody KW-0761 as a novel immunotherapeutic agent for adult T-cell leukemia\/lymphoma","volume":"16","author":"Ishii","year":"2010","journal-title":"Clin Cancer Res."},{"key":"2020070702341808300_CIT0014","doi-asserted-by":"crossref","first-page":"15036","DOI":"10.1074\/jbc.272.23.15036","article-title":"The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4","volume":"272","author":"Imai","year":"1997","journal-title":"J Biol Chem."},{"key":"2020070702341808300_CIT0015","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1074\/jbc.273.3.1764","article-title":"Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4","volume":"273","author":"Imai","year":"1998","journal-title":"J Biol Chem."},{"key":"2020070702341808300_CIT0016","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1084\/jem.20140987","article-title":"Gain-of-function CCR4 mutations in adult T cell leukemia\/lymphoma","volume":"211","author":"Nakagawa","year":"2014","journal-title":"J Exp Med."},{"key":"2020070702341808300_CIT0017","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1002\/path.4699","article-title":"CCR4 frameshift mutation identifies a distinct group of adult T cell leukaemia\/lymphoma with poor prognosis","volume":"238","author":"Yoshida","year":"2016","journal-title":"J Pathol."},{"key":"2020070702341808300_CIT0018","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1182\/blood-2018-02-835991","article-title":"CCR4 mutations associated with superior outcome of adult T-cell leukemia\/lymphoma under mogamulizumab treatment","volume":"132","author":"Sakamoto","year":"2018","journal-title":"Blood."},{"key":"2020070702341808300_CIT0019","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1093\/labmed\/lmy070","article-title":"Evaluation of SF3B1 mutation screening by high-resolution melting analysis and its clinical utility for myelodysplastic syndrome with ring sideroblasts at the point of diagnosis","volume":"50","author":"Mizuta","year":"2019","journal-title":"Lab Med."},{"key":"2020070702341808300_CIT0020","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1111\/ijlh.13056","article-title":"Investigation of screening method for DNMT3A mutations by high-resolution melting analysis in acute myeloid leukemia","volume":"41","author":"Mizuta","year":"2019","journal-title":"Int J Lab Hematol."},{"key":"2020070702341808300_CIT0021","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1182\/blood.V85.7.1954.bloodjournal8571954","article-title":"Quantitative determination of bone marrow transplant engraftment using fluorescent polymerase chain reaction primers for human identity markers","volume":"85","author":"Scharf","year":"1995","journal-title":"Blood."},{"key":"2020070702341808300_CIT0022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00277-011-1234-z","article-title":"Fragment length analysis screening for detection of CEBPA mutations in intermediate-risk karyotype acute myeloid leukemia","volume":"91","author":"Fuster","year":"2012","journal-title":"Ann Hematol."},{"key":"2020070702341808300_CIT0023","first-page":"216","article-title":"Clinical utility of measuring FLT3-ITD mutant allele burden by fragment analysis [in Japanese]","volume":"20","author":"Yamane","year":"2019","journal-title":"Int J Hematol."},{"key":"2020070702341808300_CIT0024","doi-asserted-by":"crossref","first-page":"bar018","DOI":"10.1093\/database\/bar018","article-title":"Data mining using the Catalogue of Somatic Mutations in Cancer BioMart","volume":"2011","author":"Shepherd","year":"2011","journal-title":"Database (Oxford)."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/154\/2\/236\/33470148\/aqaa037.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/154\/2\/236\/33470148\/aqaa037.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T06:34:40Z","timestamp":1594103680000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/154\/2\/236\/5829725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,5]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,5,5]]},"published-print":{"date-parts":[[2020,7,7]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqaa037","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"type":"print","value":"0002-9173"},{"type":"electronic","value":"1943-7722"}],"subject":[],"published-other":{"date-parts":[[2020,8]]},"published":{"date-parts":[[2020,5,5]]}}}