{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:19:45Z","timestamp":1740136785052,"version":"3.37.3"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>RBC alloantibodies can lead to ABO grouping discrepancies unrelated to A or B antigens or antibodies posing challenges in the blood bank testing. Routine blood bank testing and flow cytometry were used to immunophenotype reagent cells and elucidate the cause of ABO discrepancies in two patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ABO discrepancy was identified in two patients after transfusion with several units of RBCs. For both patients, the pretransfusion type and screen demonstrated blood group A. Eight and 16 days later, both patients showed an apparent antibody to reagent group A cells, which prompted additional study with patients\u2019 samples and flow cytometric testing of commercial reagent cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In both patients\u2019 specimens, posttransfusion evaluation demonstrated an emerging antibody to the Kell antigen (K). The RBCs of both patients typed negative for K, and both were transfused with K-positive RBCs. Flow cytometric analysis of reagent RBCs demonstrated that five of seven lot numbers were positive for K.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Emerging anti-K antibody led to agglutination of the K-positive reagent A1 cells, highlighting the importance of considering RBC alloantibodies and the composition of reagent cells when interpreting cases with an apparent ABO grouping discrepancy.<\/jats:p><\/jats:sec>","DOI":"10.1093\/ajcp\/aqaa117","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T19:14:44Z","timestamp":1593285284000},"page":"117-123","source":"Crossref","is-referenced-by-count":1,"title":["A Flow Cytometric Study of Reagent Cells to Resolve ABO Typing Discrepancy"],"prefix":"10.1093","volume":"155","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0901-6856","authenticated-orcid":false,"given":"Danielle L V","family":"Maracaja","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Texas Health, San Antonio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Tech University, The Hospitals of Providence, El Paso"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Salazar","sequence":"additional","affiliation":[{"name":"Department of Pathology Services, University Health System, San Antonio, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Barry","sequence":"additional","affiliation":[{"name":"Department of Pathology Services, University Health System, San Antonio, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"LaForce","sequence":"additional","affiliation":[{"name":"Bio-Rad Laboratories, Redmond, WA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Holder","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Texas Health, San Antonio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Texas Health, San Antonio"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"key":"2021010403501103300_CIT0001","doi-asserted-by":"crossref","first-page":"76","DOI":"10.21307\/immunohematology-2019-012","article-title":"Recognizing and resolving ABO discrepancies","volume":"33","author":"Meny","year":"2017","journal-title":"Immunohematology."},{"key":"2021010403501103300_CIT0002","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1309\/LMEWVSNT2F3O5JDN","article-title":"A 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