{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:26:56Z","timestamp":1761845216593,"version":"3.37.3"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/110\/409\/2011"],"award-info":[{"award-number":["E-26\/110\/409\/2011"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To evaluate the expression of progesterone receptor (PR), estrogen receptor (ER), and G protein\u2013coupled estrogen receptor 1 (GPER-1) in cutaneous neurofibromas (cNFs) and their correlation with demographic, clinical, and laboratory data of individuals with neurofibromatosis 1 (NF1). The association of PROGINS polymorphism and PR expression in cNFs, as well as the serum steroidal hormones and the number of cNFs, was investigated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The sample comprised 80 large and 80 small cNFs from 80 individuals with NF1. PR, ER, GPER-1, and Ki-67 expression were investigated by immunohistochemistry in tissue micro- and macroarrays and quantified using a digital computer-assisted method. The number of cNFs, the levels of serum 17\u03b2 estradiol and progesterone, and the PROGINS polymorphism were identified.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Twelve (8.5%) small cNFs were weakly positive for ER, 131 (92.3%) cNFs expressed PR, and all (100%) cNFs expressed GPER-1. Large cNFs showed a higher expression of PR (P &amp;lt; .0001) and GPER-1 (P = .019) and had a higher intensity of staining for these receptors (P &amp;lt; .0001). The cell proliferation index was positively correlated with PR (P = .001). Persons with more cNFs had higher serum levels of progesterone (P = .001).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These findings emphasize the role of estrogen and progesterone in cNF development and suggest that these hormones may act on cNF cells via a noncanonical pathway through GPER-1.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqaa186","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T19:11:48Z","timestamp":1598641908000},"page":"738-747","source":"Crossref","is-referenced-by-count":3,"title":["A Clinicopathologic Study on the Role of Estrogen, Progesterone, and Their Classical and Nonclassical Receptors in Cutaneous Neurofibromas of Individuals With Neurofibromatosis 1"],"prefix":"10.1093","volume":"155","author":[{"given":"Rafaela E","family":"Rozza-de-Menezes","sequence":"first","affiliation":[{"name":"Graduate Program in Pathology, School of Medicine"},{"name":"Department of Pathology, School of Medicine, Antonio Pedro University Hospital"},{"name":"Department of General and Specialized Surgery, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian M","family":"Almeida","sequence":"additional","affiliation":[{"name":"Graduate Program in Pathology, School of Medicine"},{"name":"Department of Pathology, School of Medicine, Antonio Pedro University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel M","family":"Andrade-Losso","sequence":"additional","affiliation":[{"name":"Graduate Program in Pathology, School of Medicine"},{"name":"Department of Pathology, School of Medicine, Antonio Pedro University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3897-6872","authenticated-orcid":false,"given":"Gustavo","family":"de Souza Vieira","sequence":"additional","affiliation":[{"name":"Graduate Program in Pathology, School of Medicine"},{"name":"Department of Pathology, School of Medicine, Antonio Pedro University Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlando H K","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Neurofibromatosis National Center (Centro Nacional de Neurofibromatose), Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina I","family":"Brum","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent M","family":"Riccardi","sequence":"additional","affiliation":[{"name":"The Neurofibromatosis Institute, La Crescenta, CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin S","family":"Cunha","sequence":"additional","affiliation":[{"name":"Graduate Program in Pathology, School of Medicine"},{"name":"Department of Pathology, School of Medicine, Antonio Pedro University Hospital"},{"name":"Department of General and Specialized Surgery, School of Medicine, Universidade Federal Fluminense, Niter\u00f3i, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"2021042619281960900_CIT0001","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1186\/s13023-014-0202-9","article-title":"Validity and interexaminer reliability of a new method to quantify skin neurofibromas of neurofibromatosis 1 using paper frames","volume":"9","author":"Cunha","year":"2014","journal-title":"Orphanet J Rare Dis."},{"key":"2021042619281960900_CIT0002","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1136\/jcp.56.10.758","article-title":"Identification of growth hormone receptor in 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