{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T23:34:40Z","timestamp":1776382480746,"version":"3.51.2"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T00:00:00Z","timestamp":1613779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Human kidney injury molecule 1 (hKIM-1) is a sensitive and specific marker for detection of clear cell renal cell carcinoma (CRCC), papillary renal cell carcinoma (PRCC), and ovarian clear cell carcinoma (OCCC). Its use was limited to a few surgical pathology laboratories because this specific antibody to hKIM-1 was not commercially available. We investigated the diagnostic utility of RNA in situ hybridization\/RNAscope in the detection of hKIM-1 in tumors from various organs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>RNAscope for hKIM-1 was performed on 1,252 cases on tissue microarray sections, including CRCC (n = 185), PRCC (n = 59), chromophobe renal cell carcinoma (n = 18), oncocytoma (n = 12), OCCC (n = 27), and metastatic CRCC (n = 46).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Fifty-nine (100%) of 59 PRCCs, 94 (95%) of 99 low-grade CRCCs, 83 (96%) of 86 high-grade CRCCs, and 24 (89%) of 27 OCCCs, and 44 (96%) of 46 metastatic CRCCs were positive for hKIM-1. In contrast, hKIM-1 expression was not seen in normal renal tubules or in most nonrenal tumors. Low-level expression could be seen in a small percentage of urothelial, hepatocellular, and colon carcinomas.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>hKIM-1 is a sensitive and relatively specific marker (1) for diagnosing PRCC, CRCC, and OCCC when working on a tumor of unknown origin and (2) for differentiating CRCC from chromophobe renal cell carcinoma and oncocytoma.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqaa236","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T19:11:47Z","timestamp":1603307507000},"page":"288-299","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of Human Kidney Injury Molecule 1 (hKIM-1) Expression in Tumors From Various Organs by Messenger RNA In Situ Hybridization"],"prefix":"10.1093","volume":"156","author":[{"given":"Iman","family":"Sarami","sequence":"first","affiliation":[{"name":"Geisinger Medical Center, Danville, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhui","family":"Shi","sequence":"additional","affiliation":[{"name":"Geisinger Medical Center, Danville, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Lin","sequence":"additional","affiliation":[{"name":"Geisinger Medical Center, Danville, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Geisinger Medical Center, Danville, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Monroe","sequence":"additional","affiliation":[{"name":"Advanced Cell Diagnostics, Newark, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Lin","sequence":"additional","affiliation":[{"name":"Geisinger Medical Center, Danville, PA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,2,20]]},"reference":[{"key":"2021070713341260300_CIT0001","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.eururo.2014.10.002","article-title":"International variations and trends in renal cell carcinoma incidence and mortality","volume":"67","author":"Znaor","year":"2015","journal-title":"Eur Urol."},{"key":"2021070713341260300_CIT0002","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1002\/cncr.23518","article-title":"Renal cell cancer stage migration: analysis of the National Cancer Data 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