{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T12:51:38Z","timestamp":1770555098759,"version":"3.49.0"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Adams Grant"},{"name":"Robert B. and Jean G. Adams Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Hemangiopericytomas (HPCs) and solitary fibrous tumors (SFTs) were considered two distinct entities, but a common gene fusion, NAB2-STAT6, has been identified in both. Although rare, HPCs and SFTs do metastasize, some many years later after resection. Given the extended disease-free interval, it can be difficult to determine with certainty if an HPC or SFT at a new anatomic location represents a second primary or metastatic disease.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>RNA was extracted from formalin-fixed, paraffin-embedded tissue of two patients with multiple SFT\/HPC samples. The fusion gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) and a custom-designed Archer FusionPlex panel (94 target genes) and the Illumina NextSeq 550.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We identified two patients with multiple resections for HPC\/SFT during 26 years at our institution. The first patient had a history of HPC and almost 10 years later she was diagnosed with malignant SFT. The HPC and the SFT shared the same fusion breakpoint. The second patient had multiple lesions in the brain and bone\/soft tissue over a 27-year span following a diagnosis of meningeal SFT. Three lesions from this patient shared the same fusion breakpoint.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our study demonstrated the same fusion breakpoints in primary and metastatic SFTs\/HPCs at different time points using both RT-PCR and the Archer fusion panel.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab045","type":"journal-article","created":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T12:28:16Z","timestamp":1615292896000},"page":"906-912","source":"Crossref","is-referenced-by-count":7,"title":["<i>NAB2-STAT6<\/i> Gene Fusions to Evaluate Primary\/Metastasis of Hemangiopericytoma\/Solitary Fibrous Tumors"],"prefix":"10.1093","volume":"156","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1804-4207","authenticated-orcid":false,"given":"Nirupama","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Collingwood","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Lisa","family":"Eich","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Cologne , Cologne , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyncia","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sooryanarayana","family":"Varambally","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sameer","family":"Al Diffalha","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5783-2049","authenticated-orcid":false,"given":"Shuko","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham , Birmingham, AL , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"2023021116312165900_CIT0001","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00401-013-1117-6","article-title":"Meningeal hemangiopericytoma and solitary fibrous tumors carry the NAB2-STAT6 fusion and can be diagnosed by nuclear expression of STAT6 protein","volume":"125","author":"Schweizer","year":"2013","journal-title":"Acta Neuropathol."},{"key":"2023021116312165900_CIT0002","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/nan.12397","article-title":"WHO 2016 classification: changes and advancements in the diagnosis of miscellaneous primary CNS tumours","volume":"44","author":"Sahm","year":"2018","journal-title":"Neuropathol Appl Neurobiolo."},{"key":"2023021116312165900_CIT0003","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/jnen\/nlv026","article-title":"NAB2-STAT6 gene fusion in meningeal hemangiopericytoma and solitary fibrous tumor","volume":"75","author":"Fritchie","year":"2016","journal-title":"J Neuropathol Exp Neurol."},{"key":"2023021116312165900_CIT0004","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1016\/j.prp.2017.08.011","article-title":"Molecular characterization of a series of solitary fibrous tumors, including immunohistochemical expression of STAT6 and NATB2-STAT6 fusion transcripts, using reverse transcriptase (RT)-polymerase chain reaction (PCR) technique: an Indian experience","volume":"213","author":"Rekhi","year":"2017","journal-title":"Pathol Res Pract."},{"key":"2023021116312165900_CIT0005","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.cancergen.2016.05.071","article-title":"The NAB2-STAT6 gene fusion in solitary fibrous tumor can be reliably detected by anchored multiplexed PCR for targeted next-generation sequencing","volume":"209","author":"Guseva","year":"2016","journal-title":"Cancer Genet."},{"key":"2023021116312165900_CIT0006","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1038\/ng.2823","article-title":"Activating ESR1 mutations in hormone-resistant metastatic breast cancer","volume":"45","author":"Robinson","year":"2013","journal-title":"Nat Genet."},{"key":"2023021116312165900_CIT0007","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/ng.2522","article-title":"Whole-exome sequencing identifies a recurrent NAB2-STAT6 fusion in solitary fibrous tumors","volume":"45","author":"Chmielecki","year":"2013","journal-title":"Nat Genet."},{"key":"2023021116312165900_CIT0008","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/ajcp\/aqz014","article-title":"Effects of improved DNA integrity by punch from tissue blocks as compared to pinpoint extraction from unstained slides on next-generation sequencing quality metrics","volume":"152","author":"Morlote","year":"2019","journal-title":"Am J Clin Pathol."},{"key":"2023021116312165900_CIT0009","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.prp.2018.12.027","article-title":"Setting out the frame conditions for feasible use of FFPE derived RNA","volume":"215","author":"Schmeller","year":"2019","journal-title":"Pathol Res Pract."},{"key":"2023021116312165900_CIT0010","doi-asserted-by":"crossref","first-page":"e34683","DOI":"10.1371\/journal.pone.0034683","article-title":"Effective DNA\/RNA co-extraction for analysis of microRNAs, mRNAs, and genomic DNA from formalin-fixed paraffin-embedded specimens","volume":"7","author":"Kotorashvili","year":"2012","journal-title":"PLoS One."},{"key":"2023021116312165900_CIT0011","first-page":"80","article-title":"Extrapleural solitary fibrous tumour","volume-title":"WHO Classification of Tumours of Soft Tissue and Bone","author":"Fletcher","year":"2013","edition":"4"},{"key":"2023021116312165900_CIT0012","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s00401-016-1545-1","article-title":"The 2016 World Health Organization classification of tumors of the central nervous system: a summary","volume":"131","author":"Louis","year":"2016","journal-title":"Acta Neuropathol."},{"key":"2023021116312165900_CIT0013","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s40792-019-0625-6","article-title":"Solitary fibrous tumor in the liver: case report and literature review","volume":"5","author":"Yugawa","year":"2019","journal-title":"Surg Case Rep."},{"key":"2023021116312165900_CIT0014","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1007\/s00109-019-01815-8","article-title":"Molecular changes in solitary fibrous tumor progression","volume":"97","author":"Park","year":"2019","journal-title":"J Mol Med (Berl)."},{"key":"2023021116312165900_CIT0015","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s00401-018-1952-6","article-title":"The impact of histopathology and NAB2-STAT6 fusion subtype in classification and grading of meningeal solitary fibrous tumor\/hemangiopericytoma","volume":"137","author":"Fritchie","year":"2019","journal-title":"Acta Neuropathol."},{"key":"2023021116312165900_CIT0016","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s00401-019-01968-3","article-title":"Prognostic significance of NAB2-STAT6 fusion variants and TERT promotor mutations in solitary fibrous tumors\/hemangiopericytomas of the CNS: not (yet) clear","volume":"137","author":"Vogels","year":"2019","journal-title":"Acta Neuropathol."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/156\/5\/906\/49161158\/aqab045.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/156\/5\/906\/49161158\/aqab045.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T16:31:42Z","timestamp":1676133102000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/156\/5\/906\/6291037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":16,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,6,2]]},"published-print":{"date-parts":[[2021,10,13]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqab045","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,11,1]]},"published":{"date-parts":[[2021,6,2]]}}}