{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:36:59Z","timestamp":1789015019893,"version":"build-2803163510"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T00:00:00Z","timestamp":1630368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100006459","name":"Children's Hospital Los Angeles","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Fat-soluble vitamins are measured to identify deficiencies that may lead to rickets, osteomalacia, night blindness, and reversible motor and sensory neuropathies. We present a rapid liquid chromatography\u2013mass spectrometry (LC-MS\/MS) method that simultaneously measures 25-hydroxyvitamin D3 (25[OH]D3), epi-25(OH)D3, 25(OH)D2, vitamin A, \u03b1-tocopherol, and \u03b3-tocopherol.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We mixed 100 \u00b5L serum with internal standard and extracted it by using supported liquid extraction plates. Reconstituted specimens were analyzed by LC-MS\/MS with a 10-minute gradient.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The method was linear, covering physiological levels with r2 &amp;gt; 0.99, and the total precision was less than 15% at all quality control levels. The lower limit of the measuring intervals for 25(OH)D3, epi-25(OH)D3, 25(OH)D2, vitamin A, \u03b1-tocopherol, and \u03b3-tocopherol were 4 ng\/mL, 4 ng\/mL, 4 ng\/mL, 1 \u00b5g\/dL, 0.2 \u00b5g\/mL, and 0.2 \u00b5g\/mL, respectively, with coefficient of variation of 20% or less. Recoveries were between 92% and 111% for National Institute of Standards and Technology reference materials and 81% and 122% for spike-recovery studies. Comparison studies for vitamin D total, vitamin A, and \u03b1-tocopherol demonstrated slopes between 1.04 and 1.11 and r2 between 0.94 and 0.96. Minimal matrix effect was observed for all analytes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We developed and validated a rapid LC-MS\/MS method for the simultaneous measurement of 25(OH)D3, epi-25(OH)D3, 25(OH)D2, vitamin A, \u03b1-tocopherol, and \u03b3-tocopherol.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab088","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T18:36:52Z","timestamp":1619116612000},"page":"15-22","source":"Crossref","is-referenced-by-count":5,"title":["Development and Validation of a Liquid Chromatography Mass Spectrometry Method for Simultaneous Measurement of 25(OH)D3, epi-25(OH)D3, 25(OH)D2, Vitamin A, \u03b1-Tocopherol, and \u03b3-Tocopherol"],"prefix":"10.1093","volume":"157","author":[{"given":"Yi","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Los Angeles , Los Angeles, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward Ki Yun","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Los Angeles , Los Angeles, CA , USA"},{"name":"Department of Clinical Pathology, Keck School of Medicine, University of Southern California , Los Angeles, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,8,31]]},"reference":[{"key":"2023020717304122800_CIT0001","volume-title":"Vitamin Analysis for the Health and Food Sciences.","author":"Eitenmiller","year":"2008","edition":"2nd ed"},{"key":"2023020717304122800_CIT0002","article-title":"CRC Press;","volume-title":"Laboratory Tests for the Assessment of Nutritional 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