{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T21:23:45Z","timestamp":1778102625665,"version":"3.51.4"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100010514","name":"Seattle Children\u2019s Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010514","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Forgoing biopsy for the diagnosis of celiac disease in children with tissue transglutaminase immunoglobulin A (tTG-IgA) levels greater than or equal to 10 times the upper limit of normal (\u226510\u00d7ULN) has been advocated by the European Society of Paediatric Gastroenterology, Hepatology and Nutrition.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Our retrospective study tested the specificity and positive predictive value (PPV) of the BioPlex 2200 assay (Bio-Rad Laboratories) in diagnosing celiac disease at the \u226510\u00d7ULN tTG-IgA threshold, which is \u2265150 U\/mL (negative &amp;lt;15 U\/mL). We used the tTG-IgA and duodenal biopsy results within 6 months following tTG-IgA measurements from 542 patients who had any number of duodenal biopsy fragments, of whom 165 patients had 5 or more tissue fragments. Sensitivity and specificity of the test were calculated using histology as the gold standard for Marsh class 2 and above.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>For histopathologic findings in the duodenum with Marsh 2 and higher, the specificity and PPV of the BioPlex 2200 at \u226510\u00d7ULN tTG-IgA were 99.5% and 95.4% using all biopsies and 97.9% and 94.9% for biopsies with 5 or more tissue fragments.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Should clinical considerations preclude endoscopy, the BioPlex 2200 assay at \u226510\u00d7ULN TTG-IgA could be considered highly suggestive of disease.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab104","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T19:14:34Z","timestamp":1620933274000},"page":"136-139","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of BioPlex 2200 tTG-IgA Diagnostic Performance for Serology-Based Diagnosis of Celiac Disease"],"prefix":"10.1093","volume":"157","author":[{"given":"M Cristina","family":"Pacheco","sequence":"first","affiliation":[{"name":"Department of Laboratories , Seattle, WA , USA"},{"name":"Departments of Laboratory Medicine and Pathology , Seattle, WA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Pediatric Gastroenterology and Hepatology, Seattle Children\u2019s Hospital , Seattle, WA , USA"},{"name":"Pediatrics, University of Washington , Seattle, WA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Dickerson","sequence":"additional","affiliation":[{"name":"Department of Laboratories , Seattle, WA , USA"},{"name":"Departments of Laboratory Medicine and Pathology , Seattle, WA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"key":"2023020717412825800_CIT0001","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/MPG.0b013e31821a23d0","article-title":"European Society for Pediatric Gastroenterology, Hepatology, and Nutrition guidelines for the diagnosis of coeliac disease","volume":"54","author":"Husby","year":"2012","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2023020717412825800_CIT0002","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1097\/MPG.0000000000002497","article-title":"European Society Paediatric Gastroenterology, Hepatology and Nutrition guidelines for diagnosing coeliac disease 2020","volume":"70","author":"Husby","year":"2020","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2023020717412825800_CIT0003","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1097\/MPG.0000000000001216","article-title":"NASPGHAN clinical report on the diagnosis and treatment of gluten-related disorders","volume":"63","author":"Hill","year":"2016","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2023020717412825800_CIT0004","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1038\/ajg.2014.29","article-title":"Patient perception of treatment burden is high in celiac disease compared with other common conditions","volume":"109","author":"Shah","year":"2014","journal-title":"Am J Gastroenterol."},{"key":"2023020717412825800_CIT0005","doi-asserted-by":"crossref","first-page":"e20164367","DOI":"10.1542\/peds.2016-4367","article-title":"Celiac disease and anorexia nervosa: a nationwide study","volume":"139","author":"M\u00e5rild","year":"2017","journal-title":"Pediatrics."},{"key":"2023020717412825800_CIT0006","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1053\/j.gastro.2017.04.023","article-title":"Validation of antibody-based strategies for diagnosis of pediatric celiac disease without biopsy","volume":"153","author":"Wolf","year":"2017","journal-title":"Gastroenterology."},{"key":"2023020717412825800_CIT0007","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1053\/j.gastro.2017.06.002","article-title":"Accuracy in diagnosis of celiac disease without biopsies in clinical practice","volume":"153","author":"Werkstetter","year":"2017","journal-title":"Gastroenterology."},{"key":"2023020717412825800_CIT0008","author":"Bio-Rad Laboratories.","year":"2014"},{"key":"2023020717412825800_CIT0009","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jim.2014.01.002","article-title":"Clinical evaluation of the BioPlex 2200 celiac IgA and IgG kits\u2014a novel multiplex screen incorporating an integral check for IgA deficiency","volume":"405","author":"Holding","year":"2014","journal-title":"J Immunol Methods."},{"key":"2023020717412825800_CIT0010","doi-asserted-by":"crossref","first-page":"e44","DOI":"10.1097\/PAS.0000000000001107","article-title":"Statement on best practices in the use of pathology as a diagnostic tool for celiac disease: a guide for clinicians and pathologists","volume":"42","author":"Robert","year":"2018","journal-title":"Am J Surg Pathol."},{"key":"2023020717412825800_CIT0011","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0950-3528(95)90032-2","article-title":"Morphology of the mucosal lesion in gluten sensitivity","volume":"9","author":"Marsh","year":"1995","journal-title":"Baillieres Clin Gastroenterol."},{"key":"2023020717412825800_CIT0012","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1097\/00042737-199910000-00019","article-title":"The histopathology of coeliac disease: time for a standardized report scheme for pathologists","volume":"11","author":"Oberhuber","year":"1999","journal-title":"Eur J Gastroenterol Hepatol."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/157\/1\/136\/49119513\/aqab104.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/157\/1\/136\/49119513\/aqab104.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T20:12:23Z","timestamp":1675800743000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/157\/1\/136\/6372660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,20]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,9,20]]},"published-print":{"date-parts":[[2022,1,6]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqab104","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,1,1]]},"published":{"date-parts":[[2021,9,20]]}}}