{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:12:44Z","timestamp":1787008364405,"version":"build-2736575974"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vlaanderen\u2014Agentschap Innoveren & Ondernemen","award":["HBC.2020.2173"],"award-info":[{"award-number":["HBC.2020.2173"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>In this study, the influence of several key elements of the cytologic sample workflow on DNA and RNA content was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The A549 cell line, patient-derived organoids, and pleural effusions were used to investigate the effect of (1) several collection media and delayed time to processing; (2) cytology specimens; (3) cytologic staining; and (4) formalin-fixed, paraffin-embedded (FFPE) cell block processing on nucleic acid quality and quantity as determined by fragment analyzer, Qubit analysis (Thermo Fisher Scientific), and quantitative polymerase chain reaction\u2013based analysis on the Idylla platform (Biocartis).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Alcohol-based collection media (CytoRich Red [Thermo Fisher Scientific] and EtOH95%) displayed high DNA and RNA preservation capacity, while phosphate-buffered saline and, to a lesser extent, formalin were associated with high RNA quality. Cytospin and smear cytology specimens were subject to DNA and RNA loss. Cytologic staining had no further impact on sample quality, hence destaining is not necessary. Both H&amp;E-stained and unstained FFPE sections are compatible with nucleic acid analysis, despite a strong decrease in DNA and RNA quality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Although several key elements of the cytologic sample workflow have an influence on DNA and RNA quality and quantity, the selection of these elements is also dependent on the downstream (ancillary) testing methods.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab121","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T07:08:37Z","timestamp":1624518517000},"page":"293-304","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of Cytologic Sample Preparations for Compatibility With Nucleic Acid Analysis"],"prefix":"10.1093","volume":"157","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7172-700X","authenticated-orcid":false,"given":"Laure","family":"Sorber","sequence":"first","affiliation":[{"name":"Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Claes","sequence":"additional","affiliation":[{"name":"Biocartis NV, Mechelen, Belgium"},{"name":"miDIAGNOSTICS, Heverlee,Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Zwaenepoel","sequence":"additional","affiliation":[{"name":"Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), Wilrijk, Belgium"},{"name":"Department of Pathology, University Hospital Antwerp (UZA), Edegem, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bieke","family":"Van Dorst","sequence":"additional","affiliation":[{"name":"Biocartis NV, Mechelen, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"De Winne","sequence":"additional","affiliation":[{"name":"miDIAGNOSTICS, Heverlee,Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Els","family":"Fransen","sequence":"additional","affiliation":[{"name":"Biocartis NV, Mechelen, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinier","family":"Wener","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University Hospital Antwerp (UZA), Edegem, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therese","family":"Lapperre","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University Hospital Antwerp (UZA), Edegem, Belgium"},{"name":"Laboratory of Experimental Medicine and Paediatrics, University of Antwerp, Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Lardon","sequence":"additional","affiliation":[{"name":"Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pauwels","sequence":"additional","affiliation":[{"name":"Center for Oncological Research (CORE), Integrated Personalized and Precision Oncology Network (IPPON), Wilrijk, Belgium"},{"name":"miDIAGNOSTICS, Heverlee,Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"key":"2022020822162330500_CIT0001","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 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