{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:12:44Z","timestamp":1787008364613,"version":"build-2736575974"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK15070"],"award-info":[{"award-number":["DK15070"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Thyroid hormone analog 3,5,3'-triiodothyroacetic acid (TRIAC) is effective in reducing the hypermetabolism in monocarboxylate transporter 8 (MCT8)\u2013deficient individuals. Because of the structural similarity between TRIAC and 3,3',5'-triiodothyronine (T3), we sought to investigate the degree of cross-reactivity of TRIAC with various commercially available total and free T3 assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Varying concentrations (50-1,000 ng\/dL) of TRIAC (Sigma Aldrich) were added to pooled serum and assayed for total T3 (TT3) and free T3 (FT3) on the following platforms: e602 (Roche Diagnostics), Architect (Abbott Diagnostics), Centaur (Siemens Healthcare Diagnostics), IMMULITE (Siemens Healthcare Diagnostics), DxI (Beckman Coulter), and Vitros (Ortho Clinical Diagnostics). TT3 competition assay with TRIAC was performed by adding increasing amounts of T3 to pooled serum samples that contained a constant concentration of TRIAC (250 ng\/dL).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Significant overestimation of TT3 and FT3 assays were observed across all platforms corresponding to increasing concentrations of TRIAC. The TRIAC effect at 250 ng\/dL showed a constant interference of approximately 190 ng\/dL TT3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>All commercial TT3 and FT3 assays tested in this work cross-react significantly with TRIAC. Therefore, patients undergoing TRIAC therapy should have T3 hormone response monitored using alternative nonimmunoassay-based methods to avoid misinterpretation of thyroid function profiles.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab124","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T15:10:35Z","timestamp":1624461035000},"page":"156-158","source":"Crossref","is-referenced-by-count":11,"title":["Triiodothyroacetic Acid Cross-Reacts With Measurement of Triiodothyronine (T3) on Various Immunoassay Platforms"],"prefix":"10.1093","volume":"157","author":[{"given":"Siaw Li","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Chicago , Chicago, IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Refetoff","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pediatrics and Committee on Genetics, The University of Chicago , Chicago, IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolina","family":"Babic","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical University of South Carolina , Charleston, SC , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pathology, The University of Illinois at Chicago , Chicago, IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uttam","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Mercy Hospital, and University of Missouri School of Medicine , Kansas City, MO ; USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4708-8420","authenticated-orcid":false,"given":"Kiang-Teck J","family":"Yeo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Pritzker School of Medicine, The University of Chicago , Chicago, IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"key":"2023020711034175500_CIT0001","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1086\/380999","article-title":"A novel syndrome combining thyroid and neurological abnormalities is associated with mutations in a monocarboxylate transporter gene","volume":"74","author":"Dumitrescu","year":"2004","journal-title":"Am J Hum Genet."},{"key":"2023020711034175500_CIT0002","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1016\/S0140-6736(04)17226-7","article-title":"Association between mutations in a thyroid hormone transporter and severe X-linked psychomotor retardation","volume":"364","author":"Friesema","year":"2004","journal-title":"Lancet."},{"key":"2023020711034175500_CIT0003","doi-asserted-by":"crossref","first-page":"40128","DOI":"10.1074\/jbc.M300909200","article-title":"Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter","volume":"278","author":"Friesema","year":"2003","journal-title":"J Biol Chem."},{"key":"2023020711034175500_CIT0004","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.beem.2007.03.005","article-title":"Syndromes of reduced sensitivity to thyroid hormone: genetic defects in hormone receptors, cell transporters and deiodination","volume":"21","author":"Refetoff","year":"2007","journal-title":"Best Pract Res Clin Endocrinol Metab."},{"key":"2023020711034175500_CIT0005","article-title":"Thyroid hormone transporters","volume":"41:bnz008","author":"Groeneweg","year":"2020","journal-title":"Endocr Rev."},{"key":"2023020711034175500_CIT0006","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1210\/jc.2012-2556","article-title":"Diiodothyropropionic acid (DITPA) in the treatment of MCT8 deficiency","volume":"97","author":"Verge","year":"2012","journal-title":"J Clin Endocrinol Metab."},{"key":"2023020711034175500_CIT0007","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/S2213-8587(19)30155-X","article-title":"Effectiveness and safety of the tri-iodothyronine analogue Triac in children and adults with MCT8 deficiency: an international, single-arm, open-label, phase 2 trial","volume":"7","author":"Groeneweg","year":"2019","journal-title":"Lancet Diabetes Endocrinol."},{"key":"2023020711034175500_CIT0008","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.cca.2015.12.008","article-title":"Diiodothyropropionic acid (DITPA) cross-reacts with thyroid function assays on different immunoassay platforms","volume":"453","author":"Leung","year":"2016","journal-title":"Clin Chim Acta."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/157\/2\/156\/49117442\/aqab124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/157\/2\/156\/49117442\/aqab124.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T10:18:26Z","timestamp":1675765106000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/157\/2\/156\/6372665"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,20]]},"references-count":8,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,9,20]]},"published-print":{"date-parts":[[2022,1,3]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqab124","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,2,1]]},"published":{"date-parts":[[2021,9,20]]}}}