{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T03:10:43Z","timestamp":1776049843381,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T00:00:00Z","timestamp":1634515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>CUX1 mutations have been reported in myeloid neoplasms. We aimed to characterize the mutational landscape, clonal architecture, and clinical characteristics of myeloid disorders with CUX1 variants.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We reviewed data from a targeted 62-gene panel with CUX1 variants. Variants were classified as of strong or potential clinical significance (tier I\/tier II) or of unknown significance (VUS).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>CUX1 variants were identified in 169 cases. The 49 tier I\/tier II variants were found in older patients (mean age, 71 vs 60 years old) and predominantly inactivating alterations, while the 120 VUS cases were missense mutations. Monosomy 7\/deletion 7q was more common in tier I\/tier II cases. Co-mutations were detected in 96% of tier I\/tier II cases (average, 3.7\/case) but in only 61% of VUS cases (average, 1.5\/case). Tier I\/tier II CUX1 variants tend to be subclonal to co-mutations (ASXL1, SF3B1, SRSF2, TET2). Among myeloid disorders, tier I\/tier II cases were more frequently diagnosed with myelodysplastic syndromes and had a higher number of bone marrow dysplastic lineages.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CUX1 mutations are seen with adverse prognostic features and could be a late clonal evolutional event of myeloid disorders. The differences between CUX1 tier I\/tier II and VUS underscore the importance of accurate variant classification in reporting of multigene panels.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab157","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T11:15:12Z","timestamp":1628766912000},"page":"586-594","source":"Crossref","is-referenced-by-count":4,"title":["Clinically Significant <i>CUX1<\/i> Mutations Are Frequently Subclonal and Common in Myeloid Disorders With a High Number of Co-mutated Genes and Dysplastic Features"],"prefix":"10.1093","volume":"157","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1139-2914","authenticated-orcid":false,"given":"Josephine K","family":"Dermawan","sequence":"first","affiliation":[{"name":"Robert J. Tomsich Pathology & Laboratory Medicine Institute, Cleveland Clinic , Cleveland, OH , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Wensel","sequence":"additional","affiliation":[{"name":"Robert J. Tomsich Pathology & Laboratory Medicine Institute, Cleveland Clinic , Cleveland, OH , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Visconte","sequence":"additional","affiliation":[{"name":"Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic , Cleveland, OH , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslaw P","family":"Maciejewski","sequence":"additional","affiliation":[{"name":"Department of Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic , Cleveland, OH , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0456-8085","authenticated-orcid":false,"given":"James R","family":"Cook","sequence":"additional","affiliation":[{"name":"Robert J. Tomsich Pathology & Laboratory Medicine Institute, Cleveland Clinic , Cleveland, OH , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S","family":"Bosler","sequence":"additional","affiliation":[{"name":"Robert J. 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