{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:35:35Z","timestamp":1790825735456,"version":"4.1.0"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To assess the efficacy of a method to circumvent CD20-positive antigen masking by rituximab for flow cytometry analysis of B-cell malignancies in hematology patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Mononuclear cells (MNCs) from 10 healthy individuals and 5 untreated patients with B-cell malignancies were sensitized with rituximab. Patients\u2019 diagnoses included chronic lymphocytic leukemia, hairy cell leukemia, and follicular lymphoma. MNCs were isolated by gradient density centrifugation. An EDTA\/glycine acid (EGA) elution method was used to dissociate CD20-rituximab complexes; afterwards, CD20-positive immunoreactivity was assessed by flow cytometry. A saturation curve was built based on serial dilutions of rituximab. Median fluorescent intensities of CD20-positive signals were obtained before sensitization with rituximab and after its elution with EGA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>CD20-positive signals were not detectable by flow cytometry after rituximab sensitization of B cells. CD20-sensitized vs CD20-unsensitized, CD20-sensitized vs CD20-eluted, and CD20-eluted vs CD20-negative control (NC) MNC populations exhibited statistical differences (P = .001), while CD20-sensitized vs CD20-NC populations did not (P = .499), confirming CD20 antigen masking by rituximab.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Rituximab interfered with the flow cytometry protocol for CD20 determination on normal and neoplastic B cells. The EGA method efficiently eluted rituximab, allowing for accurate identification of CD20-positive B cells.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqab176","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T03:09:21Z","timestamp":1631243361000},"page":"685-690","source":"Crossref","is-referenced-by-count":2,"title":["A Method for the Elution of Anti-CD20 With an EDTA\/Glycine Acid Solution for Accurate Immunophenotyping of B Lymphocytes Sensitized With Rituximab"],"prefix":"10.1093","volume":"157","author":[{"given":"Eli","family":"Fuentes-Ch\u00e1vez","sequence":"first","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nereida","family":"M\u00e9ndez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin M","family":"Maltez","sequence":"additional","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Salazar-Riojas","sequence":"additional","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"G\u00f3mez-Almaguer","sequence":"additional","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6804-9095","authenticated-orcid":false,"given":"Jos\u00e9 C","family":"Jaime-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Hematology Department, Internal Medicine Division, Dr Jos\u00e9 Eleuterio Gonz\u00e1lez University Hospital, School of Medicine, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey , 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myeloma","volume":"31","author":"Flores-Montero","year":"2017","journal-title":"Leukemia."},{"key":"2023020616235811900_CIT0010","doi-asserted-by":"crossref","first-page":"95","DOI":"10.21307\/immunohematology-2019-157","article-title":"EDTA glycine acid treatment of red blood cells","volume":"28","author":"Kosanke","year":"2012","journal-title":"Immunohematology."},{"key":"2023020616235811900_CIT0011","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1046\/j.1365-2141.2001.03019.x","article-title":"Loss of CD20 expression following treatment with rituximab (chimaeric monoclonal anti-CD20): a retrospective cohort analysis","volume":"114","author":"Foran","year":"2001","journal-title":"Br J Haematol."},{"key":"2023020616235811900_CIT0012","article-title":"Stoichiometry: calculations with formulas and chemical equations","volume-title":"Chemistry: The Central 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