{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T01:12:15Z","timestamp":1789866735209,"version":"4.0.1"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Recent studies have shown that trichorhinophalangeal syndrome type 1 (TRPS1) is a sensitive and specific marker that shows positive staining in breast carcinoma. We conducted this study to evaluate the role of TRPS1 immunohistochemistry (IHC) in differentiating breast primary vs tumors from other primary sites in malignant pleural effusion cytology specimens (MPECSs).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We selected 61 MPECS cases with cell block material available to analyze TRPS1 IHC staining. Of these 61 cases, 38 cases were metastatic carcinoma (MC) from a breast primary. We primarily selected MC cases confirmed as breast origin based on GATA binding protein 3 IHC positivity, except in two of the cases. The remaining 23 MPECS cases were from various primary sites, including urothelial (n = 6), M\u00fcllerian (n = 6), lung adenocarcinoma (n = 6), malignant melanoma (MM; n = 3), and squamous cell carcinoma (SqCC; n = 2).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>TRPS1 expression was observed in 35 (92%) of 38 MCs of breast origin. The staining intensity was variable, with 18 (47%) cases showing strong nuclear expression. In comparison, no TRPS1 expression was seen in any cases of urothelial carcinoma, MM, and SqCC. However, four of six M\u00fcllerian MC cases demonstrated TRPS1 expression.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>TRPS1 is a new marker that can be used in an IHC panel to investigate breast origin in MPECS.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqac066","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T19:12:57Z","timestamp":1651605177000},"page":"416-425","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of TRPS1 Expression in Pleural Effusion Cytology Specimens With Metastatic Breast Carcinoma"],"prefix":"10.1093","volume":"158","author":[{"given":"Minhua","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Stendahl","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoping","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adebowale","family":"Adeniran","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malini","family":"Harigopal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6185-6062","authenticated-orcid":false,"given":"Syed M","family":"Gilani","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yale School of Medicine , New Haven, CT , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"2022090208582293600_CIT0001","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1002\/cncy.22021","article-title":"Ancillary studies in pleural, pericardial, and peritoneal effusion cytology","volume":"126","author":"Sundling","year":"2018","journal-title":"Cancer Cytopathol."},{"key":"2022090208582293600_CIT0002","doi-asserted-by":"crossref","first-page":"373","DOI":"10.5858\/2008-132-373-AOITC","article-title":"Application of immunohistochemistry to cytology","volume":"132","author":"Fowler","year":"2008","journal-title":"Arch Pathol Lab 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A study of paired primary and metastatic urothelial carcinomas","volume":"37","author":"Zhao","year":"2013","journal-title":"Am J Surg Pathol."},{"key":"2022090208582293600_CIT0013","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1097\/PAI.0000000000000764","article-title":"Incidence and significance of GATA3 positivity in pancreatic ductal adenocarcinoma and cholangiocarcinoma","volume":"28","author":"Agostini-Vulaj","year":"2020","journal-title":"Appl Immunohistochem Mol Morphol."},{"key":"2022090208582293600_CIT0014","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1097\/PAS.0b013e3182a0218f","article-title":"GATA3: a multispecific but potentially useful marker in surgical pathology: a systematic analysis of 2500 epithelial and nonepithelial tumors","volume":"38","author":"Miettinen","year":"2014","journal-title":"Am J Surg Pathol."},{"key":"2022090208582293600_CIT0015","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1097\/JTO.0000000000000626","article-title":"Negative thyroid transcription factor 1 expression defines an unfavorable subgroup of lung adenocarcinomas","volume":"10","author":"Zhang","year":"2015","journal-title":"J Thorac Oncol."},{"key":"2022090208582293600_CIT0016","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.humpath.2018.11.005","article-title":"A combination of GATA3 and SOX10 is useful for the diagnosis of metastatic triple-negative breast cancer","volume":"85","author":"Tozbikian","year":"2019","journal-title":"Hum Pathol."},{"key":"2022090208582293600_CIT0017","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.humpath.2018.10.009","article-title":"Immunohistochemical profiles in primary lung cancers and epithelial pulmonary metastases","volume":"84","author":"Vidarsdottir","year":"2019","journal-title":"Hum Pathol."},{"key":"2022090208582293600_CIT0018","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1097\/PAI.0000000000000883","article-title":"PAX8 expression in breast cancer","volume":"29","author":"Lu","year":"2021","journal-title":"Appl Immunohistochem Mol Morphol."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/158\/3\/416\/45608549\/aqac066.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/158\/3\/416\/45608549\/aqac066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T08:58:39Z","timestamp":1662109119000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/158\/3\/416\/6618763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,27]]},"references-count":18,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,6,27]]},"published-print":{"date-parts":[[2022,9,2]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqac066","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,9,1]]},"published":{"date-parts":[[2022,6,27]]}}}