{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T16:31:30Z","timestamp":1719937890623},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Whole blood real-time polymerase chain reaction (WB-RTPCR) detection of Borrelia burgdorferi is not currently recommended for diagnosing Lyme disease. This study aims to elucidate the utility of WB-RTPCR as a diagnostic aid for early Lyme disease (ELD), defined as either positive PCR or positive immunoglobulin M with negative immunoglobulin G immunoblot.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A retrospective analysis was performed on 33,199 blood specimens evaluated concurrently by WB-RTPCR and antibody-capture serology (ACEIA) methods (group A). Fifty-six pairs of specimens from a separate data set were retrospectively identified and analyzed at initial and follow-up time points to monitor for seroconversion (group B). Also, a separate data set of 2,526 specimens concurrently assessed by molecular and modified two-tiered enzyme-linked immunosorbent assay serology methods was analyzed (group C).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Group A yielded 1,379 specimens consistent with ELD when tested by ACEIA and WB-RTPCR. In total, 131 (9.5% of positive results) were identified by WB-RTPCR, with negative serology. Group C identified 358 samples compatible with ELD, with 31 (8.7% of positive results) identified by RTPCR alone.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>When used concurrently with serologic testing, WB-RTPCR testing increases diagnostic sensitivity in cases of ELD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqac068","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T11:14:06Z","timestamp":1651749246000},"page":"327-330","source":"Crossref","is-referenced-by-count":1,"title":["Utility of Whole Blood Real-Time PCR Testing for the Diagnosis of Early Lyme Disease"],"prefix":"10.1093","volume":"158","author":[{"given":"George W","family":"Pratt","sequence":"first","affiliation":[{"name":"Quest Diagnostics, North Region , Marlborough, MA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihae","family":"Platt","sequence":"additional","affiliation":[{"name":"Quest Diagnostics, North Region , Marlborough, MA , USA"},{"name":"Athena Diagnostics , Marlborough, MA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Velez","sequence":"additional","affiliation":[{"name":"Quest Diagnostics, North Region , Marlborough, MA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lokinendi V","family":"Rao","sequence":"additional","affiliation":[{"name":"Quest Diagnostics, North Region , Marlborough, MA , USA"},{"name":"UMass Chan Medical School , Worcester, MA , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"2022090208580503800_CIT0001","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3201\/eid2702.202728","article-title":"Evaluation of commercial insurance 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multiplex high-definition PCR assay for detection of tick-borne pathogens in whole blood specimens","volume":"57","author":"Buchan","year":"2019","journal-title":"J Clin Microbiol."},{"key":"2022090208580503800_CIT0012","doi-asserted-by":"crossref","first-page":"e2355","DOI":"10.1093\/cid\/ciaa858","article-title":"Detection of Borrelia burgdorferi cell-free DNA in human plasma samples for improved diagnosis of early Lyme borreliosis","volume":"73","author":"Branda","year":"2021","journal-title":"Clin Infect Dis."},{"key":"2022090208580503800_CIT0013","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1471-2334-9-79","article-title":"Diagnostic challenges of early Lyme disease: lessons from a community case series","volume":"9","author":"Aucott","year":"2009","journal-title":"BMC Infect Dis."}],"container-title":["American Journal of Clinical 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