{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:11:18Z","timestamp":1790892678236,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T00:00:00Z","timestamp":1671840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,13]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Hirschsprung disease (HD) is a congenital condition defined by the absence of ganglion cells in the distal-most portion of the gastrointestinal tract. Biopsies and resections for HD can be adrenaline inducing for the general surgical pathologist because specimens are infrequent; HD is 1 of only a few neuroanatomic diseases that general surgical pathologists diagnose; numerous preanalytic factors (eg, biopsy adequacy, surgeon sampling protocol, processing artifacts) can affect histologic interpretation; and most importantly, the diagnosis has high stakes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We provide a comprehensive overview of the background, relevant clinical procedures, and pathologic assessment of HD. Grossing and frozen section protocols, an algorithmic approach to diagnosis, and histologic pearls and pitfalls are also discussed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Evaluation and recognition of the features of HD have evolved significantly in the past 2 decades with the discovery of the value of calretinin immunohistochemistry in the late 2000s and the recent development of straightforward and reproducible histologic criteria for identification of the HD transition zone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These advancements have substantially improved the pathologist\u2019s ability to reliably evaluate for HD. Nonetheless, as with any high-stakes surgical pathology specimen, clear communication with the clinical team is essential.<\/jats:p><\/jats:sec>","DOI":"10.1093\/ajcp\/aqac141","type":"journal-article","created":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T11:04:20Z","timestamp":1671879860000},"page":"228-241","source":"Crossref","is-referenced-by-count":23,"title":["Hirschsprung Disease for the Practicing Surgical Pathologist"],"prefix":"10.1093","volume":"159","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2268-1815","authenticated-orcid":false,"given":"Karen","family":"Matsukuma","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of California Davis School of Medicine , Sacramento, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorina","family":"Gui","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of California Davis School of Medicine , Sacramento, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Payam","family":"Saadai","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Pediatric Surgery, University of California Davis School of Medicine , Sacramento, CA , USA"},{"name":"Pediatric Colorectal Center, Shriners Hospitals for Children , Sacramento, CA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,24]]},"reference":[{"key":"2023031312253762900_","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/978-3-030-15647-3_10","article-title":"Epidemiology and clinical characteristics of Hirschsprung\u2019s disease.","volume-title":"Hirschsprung\u2019s Disease and Allied Disorders","author":"Puri","year":"2019","edition":"4"},{"key":"2023031312253762900_","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/978-3-642-54053-0_7","article-title":"Intestinal motor 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