{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:19:24Z","timestamp":1740136764295,"version":"3.37.3"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T00:00:00Z","timestamp":1691712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Mpox is a viral disease caused by monkeypox, a highly contagious orthopoxvirus that resulted in a global outbreak beginning in spring 2022. Diagnosis is confirmed via polymerase chain reaction (PCR) testing of swabs from mucocutaneous lesions. Rare reports have documented the histologic changes of mpox lesions, but the cytologic features have not been described. We present the cytology findings of samples taken from swabs of mucocutaneous mpox lesions in 3 different patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The patients were all male, aged 55, 43, and 37 years, all with mpox confirmed by PCR testing. Swabs from chest (cases 1 and 2) and tongue (case 3) lesions were directly sampled and submitted in Aptima (case 1) or PreservCyt solution (cases 2 and 3). Liquid-based preps were prepared and stained using the Papanicolaou method. Specimens were assessed for viral cytopathic changes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>All cases showed nuclear cytopathic changes (enlarged nuclei with open chromatin and prominent red nucleoli), 2 cases demonstrated multinucleated keratinocytes, and 1 case showed potential Guarnieri bodies. The chromatin margination and nuclear molding typical of herpesviruses was not appreciated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The cytopathic changes of monkeypox are not specific, but their recognition could prompt appropriate PCR testing. Monkeypox shows distinct cytologic changes compared with herpesviruses.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqad102","type":"journal-article","created":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T12:54:59Z","timestamp":1691758499000},"page":"9-15","source":"Crossref","is-referenced-by-count":0,"title":["Monkeypox virus cytologic findings: An institutional experience with an emerging threat"],"prefix":"10.1093","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0031-0001","authenticated-orcid":false,"given":"Kartik","family":"Viswanathan","sequence":"first","affiliation":[{"name":"Department of Pathology, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrina M","family":"Mansmann","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-9493","authenticated-orcid":false,"given":"Kimberly","family":"Workowski","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5610-3541","authenticated-orcid":false,"given":"Jeannette","family":"Guarner","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly M","family":"Heiman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0409-2374","authenticated-orcid":false,"given":"Daniel J","family":"Lubin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University , Atlanta, GA , US"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,8,11]]},"reference":[{"issue":"2","key":"2024010408032332000_CIT0001","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1699-0463.1959.tb00328.x","article-title":"A pox-like disease in cynomolgus monkeys","volume":"46","author":"von Magnus","year":"1959","journal-title":"Acta Pathol Microbiol Scand"},{"issue":"2","key":"2024010408032332000_CIT0002","first-page":"165","article-title":"Human monkeypox, 1970-79","volume":"58","author":"Breman","year":"1980","journal-title":"Bull World Health Organ."},{"key":"2024010408032332000_CIT0003","first-page":"5077","article-title":"Human monkeypox\u2014after 40 years, an unintended consequence of smallpox eradication","volume-title":"Vaccine.","author":"Simpson","year":"2020"},{"key":"2024010408032332000_CIT0004","first-page":"16262","article-title":"Major increase in human monkeypox incidence 30 years after smallpox vaccination campaigns cease in the Democratic Republic of Congo","volume-title":"Proc Natl Acad Sci U S A.","author":"Rimoin","year":"2010"},{"key":"2024010408032332000_CIT0005","first-page":"e0010141","article-title":"The changing epidemiology of human monkeypox\u2014a potential threat? A systematic review","volume-title":"PLoS Negl Trop Dis.","author":"Bunge","year":"2022"},{"issue":"10","key":"2024010408032332000_CIT0006","first-page":"1","article-title":"Waking up to monkeypox in the midst of COVID-19","volume":"14","author":"Jayasinghe","year":"2022","journal-title":"Cureus"},{"key":"2024010408032332000_CIT0007","first-page":"280","article-title":"Monkeypox: a review of the history and emergence in the Western Hemisphere","volume-title":"Semin Pediatr Infect Dis.","author":"Ligon","year":"2004"},{"key":"2024010408032332000_CIT0008","first-page":"872","article-title":"Outbreak of human monkeypox in Nigeria in 2017-18: a clinical and epidemiological report","volume-title":"Lancet Infect Dis.","author":"Yinka-Ogunleye","year":"2019"},{"key":"2024010408032332000_CIT0009","first-page":"102855","article-title":"The 2022 outbreak and the pathobiology of the monkeypox virus","volume-title":"J Autoimmun.","author":"Kumar","year":"2022"},{"year":"2023","author":"Centers for Disease Control and Prevention","key":"2024010408032332000_CIT0010"},{"key":"2024010408032332000_CIT0011","first-page":"679","article-title":"Monkeypox virus infection in humans across 16 countries\u2014April-June 2022","volume-title":"N Engl J Med.","author":"Thornhill","year":"2022"},{"key":"2024010408032332000_CIT0012","first-page":"e072410","article-title":"Clinical features and novel presentations of human monkeypox in a central London centre during the 2022 outbreak: descriptive case series","volume-title":"BMJ.","author":"Patel","year":"2022"},{"key":"2024010408032332000_CIT0013","first-page":"681","article-title":"Clinical evolution of monkeypox lesions","volume-title":"QJM.","author":"Ordinola Navarro","year":"2022"},{"key":"2024010408032332000_CIT0014","first-page":"1132","article-title":"Histological features associated with human monkeypox virus infection in 2022 outbreak in a non-endemic country","volume-title":"Clin Infect Dis.","author":"Chalali","year":"2022"},{"key":"2024010408032332000_CIT0015","first-page":"28","article-title":"Monkeypox virus: histologic, immunohistochemical and electron-microscopic findings","volume-title":"J Cutan Pathol.","author":"Bayer-Garner","year":"2005"},{"key":"2024010408032332000_CIT0016","first-page":"192","article-title":"The histopathology and electron microscopy of a human monkeypox lesion","volume-title":"Trans R Soc Trop Med Hyg.","author":"Stagles","year":"1985"},{"key":"2024010408032332000_CIT0017","first-page":"1581","article-title":"The pathology of experimental aerosolized monkeypox virus infection in cynomolgus monkeys (Macaca fascicularis)","volume-title":"Lab Invest.","author":"Zaucha","year":"2001"},{"key":"2024010408032332000_CIT0018","first-page":"173","article-title":"On the occurrence of Cytoryctes Variolae, Guarnieri, in the skin of the monkey inoculated with variola virus","volume-title":"J Med Res.","author":"Magrath","year":"1904"},{"key":"2024010408032332000_CIT0019","first-page":"e0133622","article-title":"Characterization of the cytopathic effects of monkeypox virus isolated from clinical specimens and differentiation from common viral exanthems","volume-title":"J Clin Microbiol.","author":"Ma","year":"2022"},{"key":"2024010408032332000_CIT0020","first-page":"139","article-title":"Monkeypox in 2022\u2014what clinicians need to know","volume-title":"JAMA.","author":"Guarner","year":"2022"},{"key":"2024010408032332000_CIT0021","first-page":"209","article-title":"Clinicopathologic features of varicella zoster virus infection of the upper gastrointestinal tract","volume-title":"Am J Surg Pathol.","author":"Mostyka","year":"2021"},{"key":"2024010408032332000_CIT0022","first-page":"1","article-title":"Herpes simplex virus and varicella-zoster virus","volume-title":"Microbiol Spectr.","author":"Levin","year":"2016"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/161\/1\/9\/54917815\/aqad102.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/161\/1\/9\/54917815\/aqad102.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T08:05:20Z","timestamp":1704355520000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/161\/1\/9\/7241303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,11]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,8,11]]},"published-print":{"date-parts":[[2024,1,4]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqad102","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"type":"print","value":"0002-9173"},{"type":"electronic","value":"1943-7722"}],"subject":[],"published-other":{"date-parts":[[2024,1,1]]},"published":{"date-parts":[[2023,8,11]]}}}