{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:00:40Z","timestamp":1782482440561,"version":"3.54.5"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Podari Zhizn Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Mixed-phenotype acute leukemia (MPAL) is a rare disease associated with difficulties in the correct lineage assignment of leukemic cells. One of the least common subtypes within this category is characterized by the simultaneous presence of B- and T-lineage\u2013defining antigens. Each case of suspected B\/T MPAL should be considered in light of all available laboratory and clinical data to avoid misdiagnosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In this study, we describe 6 pediatric patients who presented with leukemic blasts bearing B- and T-lineage antigens at diagnosis, including their clinical, immunophenotypic, morphologic, and cytogenetic characteristics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In 3 patients, more or less distinct populations of B- and T-lymphoid origin were found; the other 3 patients had a single mixed-phenotype blast population. All cases fulfilled the World Health Organization criteria, but not all of them turned out to be bona fide cases of B\/T MPAL according to the available clinical and laboratory data. Found genetic lesions were helpful for the confirmation of MPAL instead of 2 concomitant tumors, but for a general B\/T MPAL diagnosis, genetic studies provided the only descriptive data.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The accurate diagnosis of B\/T MPAL requires a multidisciplinary approach combining high-tech laboratory methods and close cooperation between treating physicians and pathologists.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqae020","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T22:16:05Z","timestamp":1711059365000},"page":"180-190","source":"Crossref","is-referenced-by-count":4,"title":["Laboratory characterization of the pediatric B\/T subtype of mixed-phenotype acute leukemia: Report of a case series"],"prefix":"10.1093","volume":"162","author":[{"given":"Irina","family":"Demina","sequence":"first","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9634-5828","authenticated-orcid":false,"given":"Elena","family":"Zerkalenkova","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Semchenkova","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Roumiantseva","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Borkovskaya","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Matveev","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"},{"name":"Institute for Information Transmission Problems (the Kharkevich Institute, RAS) , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Abramov","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Konovalov","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Miakova","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Ponomareva","sequence":"additional","affiliation":[{"name":"Republican Children\u2019s Hospital , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Belkina","sequence":"additional","affiliation":[{"name":"Republican Children\u2019s Hospital , Simferopol , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Kondratchik","sequence":"additional","affiliation":[{"name":"Morozov Children\u2019s Hospital , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia","family":"Olshanskaya","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2322-5734","authenticated-orcid":false,"given":"Galina","family":"Novichkova","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Karachunskiy","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0889-6986","authenticated-orcid":false,"given":"Alexander","family":"Popov","sequence":"additional","affiliation":[{"name":"Dmitry Rogachev National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology , Moscow , Russian 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Guidelines of Russian-Belarusian multicenter group for pediatric leukemia studies [in Russian]","volume-title":"Pediatr Hematol\/Oncol Immunopathol","author":"Popov","year":"2023"},{"key":"2024080105585764100_CIT0020","first-page":"145","article-title":"Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds","volume-title":"Cytometry A.","author":"Kalina","year":"2015"},{"key":"2024080105585764100_CIT0021","first-page":"64","article-title":"Standardization of flow cytometric minimal residual disease monitoring in children with B-cell precursor acute lymphoblastic leukemia. 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