{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T22:58:24Z","timestamp":1783810704679,"version":"3.55.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T00:00:00Z","timestamp":1746835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21NS119660"],"award-info":[{"award-number":["R21NS119660"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Many fungal species share overlapping morphologic features in tissue sections, preventing reliable identification and optimal treatment. We sought to determine whether immunohistochemistry (IHC) using a panel of commercially available antibodies could effectively distinguish between fungi commonly encountered in anatomic pathology specimens.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Anti-Aspergillus, anti-Rhizopus, and anti-Candida IHC was performed on formalin-fixed, paraffin-embedded tissue sections from 24 cases with fungal infections identified by culture or sequencing (including 4 polyfungal infections).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Anti-Aspergillus IHC was positive in 6 of 6 Aspergillus and focally in 1 of 4 Candida species infections and negative in all cases of Fusarium, Scedosporium, Rhizopus, and Mucor species, yielding overall sensitivity of 100% and specificity of 95%. Anti-Rhizopus IHC was positive in 4 of 4 Rhizopus and 1 of 3 Mucor species infections and negative in all other cases, with a sensitivity of 71% and a specificity of 100%. Anti-Candida IHC was positive in 4 of 4 Candida species infections and showed some cross-reactivity in all other cases, resulting in 100% sensitivity and 0% specificity.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Anti-Aspergillus IHC was highly sensitive and specific in its ability to distinguish Aspergillus from other similar-appearing hyaline molds, including Fusarium and Scedosporium species. Anti-Rhizopus IHC was moderately sensitive and highly specific, while anti-Candida IHC was highly sensitive but had minimal specificity.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqaf037","type":"journal-article","created":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T08:57:54Z","timestamp":1746867474000},"page":"216-225","source":"Crossref","is-referenced-by-count":2,"title":["Sensitivity and specificity of immunohistochemistry for the diagnosis of filamentous fungal infections"],"prefix":"10.1093","volume":"164","author":[{"given":"Victoria L","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Pathology, Brigham and Women\u2019s Hospital, Harvard Medical School , Boston, MA ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1282-2274","authenticated-orcid":false,"given":"Alvaro C","family":"Laga","sequence":"additional","affiliation":[{"name":"Department of 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