{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T23:58:53Z","timestamp":1780876733784,"version":"3.54.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Determination of B-cell clonality in formalin-fixed, paraffin-embedded tissues is challenging, especially in cases where tumor B cells are sparse. Flow cytometry is of limited utility in Hodgkin lymphomas, at least in part due to the rarity and fragility of the malignant cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used a recently US Food and Drug Administration\u2013approved, ultrasensitive \u03ba and \u03bb dual in situ hybridization assay (K\/L dual ISH; Roche\/Ventana Medical Systems) to evaluate B-cell clonality in 184 classic Hodgkin lymphomas (CHLs) and 113 nodular lymphocyte-predominant Hodgkin lymphomas (NLPHLs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Five CHLs and 11 NLPHLs were not scorable due to preanalytical variables. Of the 179 scorable CHL cases, none showed light chain messenger RNA expression in the Hodgkin\/Reed-Sternberg cells, whereas 89% of NLPHLs (91\/102) exhibited light chain expression in lymphocyte-predominant (LP) cells. Among NLPHLs, 66% (68\/102) showed \u03ba restriction, 20% (21\/102) showed \u03bb restriction, and 1.9% (2\/102) showed dual \u03ba and \u03bb expression in the LP cells. There was no appreciable light chain expression in 10% (11\/102) of cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Although morphology and immunohistochemistry are usually sufficient to separate CHL from NLPHL, determining light chain restriction by K\/L dual ISH could provide a valuable adjunct in challenging cases, potentially improving diagnostic accuracy and patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ajcp\/aqag043","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T11:48:39Z","timestamp":1775735319000},"source":"Crossref","is-referenced-by-count":0,"title":["Utility of ultrasensitive, US Food and Drug Administration\u2013approved \u03ba and \u03bb dual in situ hybridization assay in the diagnosis of Hodgkin lymphomas"],"prefix":"10.1093","volume":"165","author":[{"given":"Mohamed","family":"Eltahir","sequence":"first","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine , Stanford, CA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine , Stanford, CA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheren","family":"Younes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine , Stanford, CA,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuchun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine , Stanford, CA,","place":["United 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