{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:34:22Z","timestamp":1788975262328,"version":"build-2803163510"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Lacrimal gland adenoid cystic carcinoma (ACC) is a rare and lethal malignancy associated with a poor prognosis, with no known therapeutic targets. The clinical relevance of Myb, Sox4, c-Kit protein expression, and the MYB::NFIB fusion gene in lacrimal gland ACC is not well known. The current study aims to investigate the prognostic significance of Myb, Sox-4, c-Kit protein, and the MYB::NFIB fusion gene in lacrimal gland ACC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 30 histopathologically diagnosed lacrimal gland ACC cases were included in this study. Myb, Sox4, c-Kit expression, and MYB::NFIB gene fusion were evaluated by immunohistochemistry and fluorescence in situ hybridization, respectively. The results were correlated with clinicopathologic features and patient survival.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Immunoexpression of Myb, Sox4, and c-Kit was observed in 70%, 63%, and 63% of ACC cases, respectively. Myb expression showed a significant association with the solid and cribriform histologic patterns, Sox-4 expression, and disease-free survival. On univariate analysis, Myb expression, solid histologic pattern, bone erosion, intracranial extension, and advanced tumor stage were indicators of poor prognosis. c-Kit expression showed a significant association with advanced tumor stage. MYB::NFIB fusion, detected in 47% of cases, was significantly associated with intracranial extension.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Myb protein emerged as a prognostic indicator in the pathogenesis and clinical outcomes of patients with lacrimal gland ACC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ajcp\/aqag073","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T11:45:44Z","timestamp":1779968744000},"source":"Crossref","is-referenced-by-count":1,"title":["Prognostic significance of Myb protein and its downstream target genes in lacrimal gland adenoid cystic carcinoma"],"prefix":"10.1093","volume":"165","author":[{"given":"Shahzan","family":"Anjum","sequence":"first","affiliation":[{"name":"Ocular Pathology Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Sen","sequence":"additional","affiliation":[{"name":"Ocular Pathology Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehar C","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pathology, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer","family":"Bakhshi","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunzang","family":"Chosdol","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Ocular Pathology Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Pushker","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandeep S","family":"Bajaj","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachna","family":"Meel","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,27]]},"reference":[{"key":"2026062711312500800_aqag073-B1","doi-asserted-by":"publisher","first-page":"4444","DOI":"10.1002\/cncr.27408","article-title":"Adenoid cystic carcinoma of the head and neck: incidence and survival trends based on 1973-2007 Surveillance, Epidemiology, and End Results data","volume":"118","author":"Ellington","year":"2012","journal-title":"Cancer"},{"key":"2026062711312500800_aqag073-B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/aos.12271","article-title":"Tumors of the lacrimal gland. Epidemiological, clinical and genetic characteristics","volume":"91 Thesis 6","author":"von Holstein","year":"2013","journal-title":"Acta Ophthalmol"},{"key":"2026062711312500800_aqag073-B3","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1097\/IOP.0b013e31829f3a0c","article-title":"Clinicopathologic review of epithelial tumors of the lacrimal gland","volume":"29","author":"Chawla","year":"2013","journal-title":"Ophthalmic Plast Reconstr Surg"},{"key":"2026062711312500800_aqag073-B4","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1001\/archopht.116.5.613","article-title":"Malignant epithelial tumors of the lacrimal gland: a clinicopathologic study of 21 cases","volume":"116","author":"Font","year":"1998","journal-title":"Arch Ophthalmol"},{"key":"2026062711312500800_aqag073-B5","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1016\/S1470-2045(10)70245-X","article-title":"Systemic therapy in the management of metastatic or locally recurrent adenoid cystic carcinoma of the salivary glands: a systematic review","volume":"12","author":"Laurie","year":"2011","journal-title":"Lancet Oncol"},{"key":"2026062711312500800_aqag073-B6","doi-asserted-by":"publisher","first-page":"3945","DOI":"10.1002\/cncr.26740","article-title":"A comparison of the demographics, clinical features, and survival of patients with adenoid cystic carcinoma of major and minor salivary glands versus less common sites within the Surveillance, Epidemiology, and End Results registry","volume":"118","author":"Li","year":"2012","journal-title":"Cancer"},{"key":"2026062711312500800_aqag073-B7","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1242\/bio.20123152","article-title":"Duplication and maintenance of the Myb genes of vertebrate animals","volume":"2","author":"Davidson","year":"2013","journal-title":"Biol Open"},{"key":"2026062711312500800_aqag073-B8","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1038\/310249a0","article-title":"Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia","volume":"310","author":"Gonda","year":"1984","journal-title":"Nature"},{"key":"2026062711312500800_aqag073-B9","doi-asserted-by":"publisher","first-page":"12901","DOI":"10.1038\/s41598-021-92352-3","article-title":"Clinicopathologic significance and race-specific prognostic association of MYB overexpression in ovarian cancer","volume":"11","author":"Miree","year":"2021","journal-title":"Sci Rep"},{"key":"2026062711312500800_aqag073-B10","doi-asserted-by":"publisher","first-page":"2868","DOI":"10.1158\/2159-8290.CD-20-1793","article-title":"Selective requirement of MYB for oncogenic hyperactivation of a translocated enhancer in leukemia","volume":"11","author":"Smeenk","year":"2021","journal-title":"Cancer Discov"},{"key":"2026062711312500800_aqag073-B11","doi-asserted-by":"publisher","first-page":"4722","DOI":"10.1158\/1078-0432.CCR-10-0463","article-title":"Comprehensive analysis of the MYB-NFIB gene fusion in salivary adenoid cystic carcinoma: incidence, variability, and clinicopathologic significance","volume":"16","author":"Mitani","year":"2010","journal-title":"Clin Cancer Res"},{"key":"2026062711312500800_aqag073-B12","doi-asserted-by":"publisher","first-page":"18740","DOI":"10.1073\/pnas.0909114106","article-title":"Recurrent fusion of MYB and NFIB transcription factor genes in carcinomas of the breast and head and neck","volume":"106","author":"Persson","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026062711312500800_aqag073-B13","doi-asserted-by":"publisher","first-page":"103745","DOI":"10.1016\/j.critrevonc.2022.103745","article-title":"MYB-NFIB fusion transcript in adenoid cystic carcinoma: current state of knowledge and future directions","volume":"176","author":"Wagner","year":"2022","journal-title":"Crit Rev Oncol Hematol"},{"key":"2026062711312500800_aqag073-B14","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1158\/2159-8290.CD-15-0859","article-title":"Recurrent fusions in MYB and MYBL1 define a common, transcription factor-driven oncogenic pathway in salivary gland adenoid cystic carcinoma","volume":"6","author":"Brayer","year":"2016","journal-title":"Cancer Discov"},{"key":"2026062711312500800_aqag073-B15","doi-asserted-by":"publisher","first-page":"7341","DOI":"10.18632\/oncotarget.23641","article-title":"Transcriptomes define distinct subgroups of salivary gland adenoid cystic carcinoma with different driver mutations and outcomes","volume":"9","author":"Frerich","year":"2018","journal-title":"Oncotarget"},{"key":"2026062711312500800_aqag073-B16","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1152\/physrev.00046.2011","article-title":"Stem cell factor receptor\/c-Kit: from basic science to clinical implications","volume":"92","author":"Lennartsson","year":"2012","journal-title":"Physiol Rev"},{"key":"2026062711312500800_aqag073-B17","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.1097\/01.MP.0000096046.42833.C7","article-title":"Expression of KIT (CD117) in neoplasms of the head and neck: an ancillary marker for adenoid cystic carcinoma","volume":"16","author":"Mino","year":"2003","journal-title":"Mod Pathol"},{"key":"2026062711312500800_aqag073-B18","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1593\/tlo.08127","article-title":"TRK-A, HER-2\/neu, and KIT expression\/activation profiles in salivary gland carcinoma","volume":"1","author":"Negri","year":"2008","journal-title":"Transl Oncol"},{"key":"2026062711312500800_aqag073-B19","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.stem.2012.12.007","article-title":"The Sox family of transcription factors: versatile regulators of stem and progenitor cell fate","volume":"12","author":"Sarkar","year":"2013","journal-title":"Cell Stem Cell"},{"key":"2026062711312500800_aqag073-B20","doi-asserted-by":"publisher","first-page":"5235","DOI":"10.3390\/cancers15215235","article-title":"Pan-cancer analysis and experimental validation of SOX4 as a potential diagnosis, prognosis, and immunotherapy biomarker","volume":"15","author":"Deng","year":"2023","journal-title":"Cancers (Basel)"},{"key":"2026062711312500800_aqag073-B21","doi-asserted-by":"publisher","first-page":"e22910","DOI":"10.1002\/jbt.22910","article-title":"SOX4 induces drug resistance of colorectal cancer cells by downregulating CYLD through transcriptional activation of microRNA-17","volume":"36","author":"Pan","year":"2022","journal-title":"J Biochem Mol Toxicol"},{"key":"2026062711312500800_aqag073-B22","doi-asserted-by":"publisher","first-page":"1315","DOI":"10.1016\/S0002-9440(10)64408-2","article-title":"Large scale molecular analysis identifies genes with altered expression in salivary adenoid cystic carcinoma","volume":"161","author":"Frierson","year":"2002","journal-title":"Am J Pathol"},{"key":"2026062711312500800_aqag073-B23","doi-asserted-by":"publisher","first-page":"93","DOI":"10.3322\/caac.21388","article-title":"The eighth edition AJCC cancer staging manual: continuing to build a bridge from a population-based to a more \u201cpersonalized\u201d approach to cancer staging","volume":"67","author":"Amin","year":"2017","journal-title":"CA Cancer J Clin"},{"key":"2026062711312500800_aqag073-B24","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1097\/PAS.0b013e3182002777","article-title":"MYB expression and translocation in adenoid cystic carcinomas and other salivary gland tumors with clinicopathologic correlation","volume":"35","author":"West","year":"2011","journal-title":"Am J Surg Pathol"},{"key":"2026062711312500800_aqag073-B25","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1186\/s12935-020-01568-2","article-title":"SOX4 promotes the growth and metastasis of breast cancer","volume":"20","author":"Zhang","year":"2020","journal-title":"Cancer Cell Int"},{"key":"2026062711312500800_aqag073-B26","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1007\/s00106-007-1666-x","article-title":"Prognostic value of immunohistochemistry in salivary gland cancer","volume":"56","author":"Ettl","year":"2008","journal-title":"HNO"},{"key":"2026062711312500800_aqag073-B27","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1038\/s41379-018-0005-y","article-title":"Adenoid cystic carcinomas of the salivary gland, lacrimal gland, and breast are morphologically and genetically similar but have distinct microRNA expression profiles","volume":"31","author":"Andreasen","year":"2018","journal-title":"Mod Pathol"},{"key":"2026062711312500800_aqag073-B28","first-page":"113","article-title":"The Myb oncogene family of transcription factors: potent regulators of hematopoietic cell proliferation and differentiation","volume":"5","author":"Introna","year":"1994","journal-title":"Semin Cancer Biol"},{"key":"2026062711312500800_aqag073-B29","doi-asserted-by":"publisher","first-page":"1694","DOI":"10.1038\/bjc.2015.400","article-title":"MYB is a novel regulator of pancreatic tumour growth and metastasis","volume":"113","author":"Srivastava","year":"2015","journal-title":"Br J Cancer"},{"key":"2026062711312500800_aqag073-B30","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1038\/modpathol.2011.86","article-title":"Analysis of MYB expression and MYB-NFIB gene fusions in adenoid cystic carcinoma and other salivary neoplasms","volume":"24","author":"Brill","year":"2011","journal-title":"Mod Pathol"},{"key":"2026062711312500800_aqag073-B31","doi-asserted-by":"publisher","first-page":"106763","DOI":"10.1016\/j.oraloncology.2024.106763","article-title":"MYB alternative promoter activity is increased in adenoid cystic carcinoma metastases and is associated with a specific gene expression signature","volume":"151","author":"Huang","year":"2024","journal-title":"Oral Oncol"},{"key":"2026062711312500800_aqag073-B32","doi-asserted-by":"publisher","first-page":"2130","DOI":"10.1016\/j.ophtha.2013.03.030","article-title":"Adenoid cystic carcinoma of the lacrimal gland: MYB gene activation, genomic imbalances, and clinical characteristics","volume":"120","author":"von Holstein","year":"2013","journal-title":"Ophthalmology"},{"key":"2026062711312500800_aqag073-B33","doi-asserted-by":"publisher","first-page":"E292","DOI":"10.1002\/lary.25356","article-title":"MYB rearrangement and clinicopathologic characteristics in head and neck adenoid cystic carcinoma","volume":"125","author":"Rettig","year":"2015","journal-title":"Laryngoscope"},{"key":"2026062711312500800_aqag073-B34","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1111\/his.13281","article-title":"MYB, MYBL1, MYBL2 and NFIB gene alterations and MYC overexpression in salivary gland adenoid cystic carcinoma","volume":"71","author":"Fujii","year":"2017","journal-title":"Histopathology"},{"key":"2026062711312500800_aqag073-B35","doi-asserted-by":"publisher","first-page":"925","DOI":"10.1111\/his.15040","article-title":"Molecular characterisation of tumours of the lacrimal apparatus","volume":"83","author":"Vibert","year":"2023","journal-title":"Histopathology"},{"key":"2026062711312500800_aqag073-B36","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/j.oraloncology.2010.02.001","article-title":"Expression of c-kit and Slug correlates with invasion and metastasis of salivary adenoid cystic carcinoma","volume":"46","author":"Tang","year":"2010","journal-title":"Oral Oncol"},{"key":"2026062711312500800_aqag073-B37","doi-asserted-by":"publisher","first-page":"2159","DOI":"10.1007\/s00432-015-1989-z","article-title":"Expression profiling of receptor tyrosine kinases in high-grade neuroendocrine carcinoma of the lung: a comparative analysis with adenocarcinoma and squamous cell carcinoma","volume":"141","author":"Matsumura","year":"2015","journal-title":"J Cancer Res Clin Oncol"},{"key":"2026062711312500800_aqag073-B38","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1038\/ng.2764","article-title":"The Cancer Genome Atlas Pan-Cancer analysis project","volume":"45","author":"Weinstein","year":"2013","journal-title":"Nat Genet"},{"key":"2026062711312500800_aqag073-B39","doi-asserted-by":"publisher","first-page":"5626","DOI":"10.1038\/sj.onc.1209566","article-title":"Knockdown of Sox4 expression by RNAi induces apoptosis in ACC3 cells","volume":"25","author":"Pramoonjago","year":"2006","journal-title":"Oncogene"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/165\/6\/aqag073\/68632759\/aqag073.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/165\/6\/aqag073\/68632759\/aqag073.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T15:31:36Z","timestamp":1782574296000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/doi\/10.1093\/ajcp\/aqag073\/8719532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6,4]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqag073","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,6]]},"published":{"date-parts":[[2026,6]]},"article-number":"aqag073"}}