{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:24:35Z","timestamp":1782577475148,"version":"3.54.5"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To examine the immunohistochemical and ultrastructural features of hepatocellular cytoplasmic globules in venous outflow impairment (VOI).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Sixty-four liver core biopsies were screened. Patients with \u03b1-1 antitrypsin (AAT) deficiency were excluded. All biopsies were stained with H&amp;E, Masson trichrome, periodic acid-Schiff with diastase digestion (PAS-D), phosphotungstic acid hematoxylin (PTAH), complement protein 4d (C4d) immunostain, and AAT immunostain. Electron microscopy was also performed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Hepatocellular globules were identified in 8% of in-house cases. Causes of VOI included heart failure and Budd-Chiari syndrome. The hepatocellular cytoplasmic globules showed size variability, random distribution, and positivity for PAS-D, PTAH, and AAT. C4d was inconsistently positive. Electron microscopy showed that the globules were lysosome-bound inclusions containing microfibrillar material and fibrinogen.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>PAS-D\u2013positive hepatocellular globules occur in VOI. They cross-react with AAT but have different appearance, localization, and ultrastructural composition from globules in AAT deficiency.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqz068","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T15:14:47Z","timestamp":1557414887000},"page":"563-569","source":"Crossref","is-referenced-by-count":6,"title":["Immunohistochemical and Ultrastructural Features of Hepatocellular Cytoplasmic Globules in Venous Outflow Impairment"],"prefix":"10.1093","volume":"152","author":[{"given":"Alessia","family":"Buglioni","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung-Teh","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taofic","family":"Mounajjed","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,3]]},"reference":[{"key":"2019100712583361200_CIT0001","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1056\/NEJM197212212872502","article-title":"Liver disease in adults with alpha-1-antitrypsin deficiency","volume":"287","author":"Berg","year":"1972","journal-title":"N Engl J Med."},{"key":"2019100712583361200_CIT0002","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1136\/jcp.30.2.132","article-title":"Alpha-1-antitrypsin bodies in the liver","volume":"30","author":"Blenkinsopp","year":"1977","journal-title":"J Clin 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with special reference to hyaline globules","volume":"162","author":"Shibayama","year":"1990","journal-title":"J Pathol."},{"key":"2019100712583361200_CIT0010","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1097\/01.pas.0000208896.92988.fc","article-title":"Glycogen pseudoground glass change in hepatocytes","volume":"30","author":"Wisell","year":"2006","journal-title":"Am J Surg Pathol."},{"key":"2019100712583361200_CIT0011","doi-asserted-by":"crossref","first-page":"568","DOI":"10.2119\/molmed.2010.00146","article-title":"Novel aspects of fibrin(ogen) fragments during inflammation","volume":"17","author":"Jennewein","year":"2011","journal-title":"Mol Med."}],"container-title":["American Journal of Clinical 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