{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T13:05:49Z","timestamp":1773407149683,"version":"3.50.1"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T00:00:00Z","timestamp":1563840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Disaccharidase (DS) activity in duodenal biopsy specimens is the gold standard for diagnosing DS deficiency. We investigated strategies to reduce the need for DS testing and whether clinical or histopathologic factors predict DS deficiency.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A retrospective chart review analyzed 1,678 DS results in children, biopsy indication(s), and duodenal histopathology.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>One or more DSs were abnormal in 42.8%. Sufficient lactase predicted sucrase, palatinase, and maltase sufficiency (negative predictive value 97.7%). Three patients had sucrase-isomaltase deficiency (0.2%). DS deficiency was more common in biopsy specimens for positive celiac serology (78.0%). Villous blunting, intraepithelial lymphocytosis, and active inflammation predicted DS deficiency; a combination of any two had an 81.4% positive predictive value.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Utilization could be reduced by only testing cases with normal duodenal histopathology and ongoing clinical suspicion for DS deficiency after reviewing pathology. In cases with suspected celiac disease and\/or mucosal injury, DS deficiency is common and likely secondary, limiting test utility.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqz091","type":"journal-article","created":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T15:12:25Z","timestamp":1559229145000},"page":"742-746","source":"Crossref","is-referenced-by-count":5,"title":["Clinical and Histopathologic Predictors of Disaccharidase Deficiency in Duodenal Biopsy Specimens"],"prefix":"10.1093","volume":"152","author":[{"given":"Robyn C","family":"Reed","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Children\u2019s Hospitals and Clinics of Minnesota, Minneapolis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Cristina","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology & Immunology, Division of Pediatric Pathology, Vanderbilt University Medical Center, Nashville, TN"},{"name":"Department of Laboratories, Seattle Children\u2019s Hospital, Seattle, WA"},{"name":"Department of Pathology, University of Washington, Seattle"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,7,23]]},"reference":[{"key":"2019110501060268400_CIT0001","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.gtc.2012.06.003","article-title":"Diarrhea caused by carbohydrate malabsorption","volume":"41","author":"Hammer","year":"2012","journal-title":"Gastroenterol Clin North Am."},{"key":"2019110501060268400_CIT0002","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4292\/wjgpt.v7.i2.283","article-title":"Disaccharidase activity in children undergoing esophagogastroduodenoscopy: a systematic review","volume":"7","author":"Daileda","year":"2016","journal-title":"World J Gastrointest Pharmacol Ther."},{"key":"2019110501060268400_CIT0003","doi-asserted-by":"crossref","first-page":"e348","DOI":"10.1111\/j.1440-1746.2007.05248.x","article-title":"Brush border enzyme activities in relation to histological lesion in pediatric celiac disease","volume":"23","author":"Prasad","year":"2008","journal-title":"J Gastroenterol Hepatol."},{"key":"2019110501060268400_CIT0004","doi-asserted-by":"crossref","first-page":"185","DOI":"10.5152\/tjg.2014.3994","article-title":"Disaccharidase activity in children with inflammatory bowel disease","volume":"25","author":"Wiecek","year":"2014","journal-title":"Turk J Gastroenterol."},{"key":"2019110501060268400_CIT0005","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1136\/jcp.37.3.298","article-title":"Intestinal lactase, sucrase, and alkaline phosphatase in 373 patients with coeliac disease","volume":"37","author":"O\u2019Grady","year":"1984","journal-title":"J Clin Pathol."},{"key":"2019110501060268400_CIT0006","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0003-2697(64)90115-0","article-title":"Method for assay of intestinal disaccharidases","volume":"7","author":"Dahlqvist","year":"1964","journal-title":"Anal Biochem."},{"key":"2019110501060268400_CIT0007","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1097\/01.mpg.0000421401.57633.90","article-title":"Clinical aspects and treatment of congenital sucrase-isomaltase deficiency","volume":"55","author":"Treem","year":"2012","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2019110501060268400_CIT0008","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1097\/01.mpg.0000421405.42386.64","article-title":"Frequency of sucrase deficiency in mucosal biopsies","volume":"55","author":"Nichols","year":"2012","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2019110501060268400_CIT0009","doi-asserted-by":"crossref","first-page":"S56","DOI":"10.1097\/MPG.0000000000001961","article-title":"Clinical characteristics of disaccharidase deficiencies among children undergoing upper endoscopy","volume":"66","author":"Cohen","year":"2018","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2019110501060268400_CIT0010","first-page":"26","article-title":"Variable use of disaccharidase assays when evaluating abdominal pain","volume":"14","author":"Cohen","year":"2018","journal-title":"Gastroenterol Hepatol (N Y)."},{"key":"2019110501060268400_CIT0011","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1097\/MPG.0b013e318231eb30","article-title":"Comparison of quick point-of-care test for small-bowel hypolactasia with biochemical lactase assay in children","volume":"54","author":"Rao","year":"2012","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"2019110501060268400_CIT0012","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1055\/s-2006-925354","article-title":"A biopsy-based quick test in the diagnosis of duodenal hypolactasia in upper gastrointestinal endoscopy","volume":"38","author":"Kuokkanen","year":"2006","journal-title":"Endoscopy."},{"key":"2019110501060268400_CIT0013","unstructured":"Carpentieri\n              DF\n            , MarshWM, McOmberE. A cost effective approach for screening lactase deficiency in a pediatric hospital [abstract]. Paper presented at: Society for Pediatric Spring Meeting; March 21-22, 2015; Boston, MA. Abstract 42."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/152\/6\/742\/30380348\/aqz091.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/152\/6\/742\/30380348\/aqz091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T01:17:48Z","timestamp":1572916668000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/152\/6\/742\/5537012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,23]]},"references-count":13,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,7,23]]},"published-print":{"date-parts":[[2019,11,4]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqz091","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,12]]},"published":{"date-parts":[[2019,7,23]]}}}