{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T08:38:24Z","timestamp":1758703104797},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T00:00:00Z","timestamp":1579305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Lymphoid enhancer binding factor 1 (LEF1) is expressed in most cases of chronic lymphocytic leukemia\/small lymphocytic lymphoma (CLL\/SLL) and has shown utility in distinguishing CLL\/SLL from other small B-cell lymphomas. LEF1 expression has not been systematically studied in CD5-positive marginal zone lymphomas (MZLs), lymphoplasmacytic lymphomas (LPLs), and follicular lymphomas (FLs). We evaluated whether these cases lacked LEF1, helping to distinguish them from CLL\/SLL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>MZLs, LPLs, and FLs expressing CD5 were retrospectively studied for expression of LEF1 by immunohistochemistry.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>LEF1 was absent in 17 of 18 CD5-positive lymphomas including 13 MZLs (2 nodal, 3 splenic, and 8 mucosa-associated lymphoid tissue lymphomas), 3 LPLs, and 1 of 2 FLs. One grade 3A CD5-positive FL expressed LEF1 in a majority of tumor cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>LEF1 is not expressed in most CD5-positive MZLs and LPLs; therefore, it is a reliable marker for distinguishing them from CLL\/SLL. LEF1 may be expressed in CD5-positive FLs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajcp\/aqz207","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T12:08:09Z","timestamp":1575374889000},"page":"646-655","source":"Crossref","is-referenced-by-count":12,"title":["Immunohistochemical Expression of Lymphoid Enhancer Binding Factor 1 in CD5-Positive Marginal Zone, Lymphoplasmacytic, and Follicular Lymphomas"],"prefix":"10.1093","volume":"153","author":[{"given":"Nisha","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Durkin","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juraj","family":"Bodo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric D","family":"Hsi","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,1,18]]},"reference":[{"key":"2020041521554117000_CIT0001","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1038\/modpathol.2011.103","article-title":"Nuclear overexpression of lymphoid-enhancer-binding factor 1 identifies chronic lymphocytic leukemia\/small lymphocytic lymphoma in small B-cell lymphomas","volume":"24","author":"Tandon","year":"2011","journal-title":"Mod Pathol."},{"key":"2020041521554117000_CIT0002","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1136\/jclinpath-2015-202862","article-title":"LEF1: a highly specific marker for the diagnosis of chronic lymphocytic B cell leukaemia\/small lymphocytic B cell lymphoma","volume":"68","author":"Menter","year":"2015","journal-title":"J Clin Pathol."},{"key":"2020041521554117000_CIT0003","doi-asserted-by":"crossref","DOI":"10.1093\/ajcp\/aqw208","article-title":"Diagnostic utility of lymphoid enhancer binding factor 1 immunohistochemistry in small B-cell lymphomas","author":"Menter","year":"2017","journal-title":"Am J Clin Pathol."},{"key":"2020041521554117000_CIT0004","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1016\/j.ajpath.2012.12.013","article-title":"Aberrant lymphocyte enhancer-binding factor 1 expression is characteristic for sporadic Burkitt\u2019s lymphoma","volume":"182","author":"Walther","year":"2013","journal-title":"Am J Pathol."},{"key":"2020041521554117000_CIT0005","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow","year":"2017"},{"key":"2020041521554117000_CIT0006","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1309\/AJCP6ZODWV1CIDME","article-title":"Lymphoplasmacytic lymphoma and marginal zone lymphoma in the bone marrow: paratrabecular involvement as an important distinguishing feature","volume":"143","author":"Bassarova","year":"2015","journal-title":"Am J Clin Pathol."},{"key":"2020041521554117000_CIT0007","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/modpathol.2015.42","article-title":"CD5-positive follicular lymphoma: clinicopathologic correlations and outcome in 88 cases","volume":"28","author":"Li","year":"2015","journal-title":"Mod Pathol."},{"key":"2020041521554117000_CIT0008","doi-asserted-by":"crossref","first-page":"604","DOI":"10.3324\/haematol.2009.011049","article-title":"CD5 expression identifies a subset of splenic marginal zone lymphomas with higher lymphocytosis: a clinico-pathological, cytogenetic and molecular study of 24 cases","volume":"95","author":"Baseggio","year":"2010","journal-title":"Haematologica."},{"key":"2020041521554117000_CIT0009","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1046\/j.1365-2141.1996.d01-1711.x","article-title":"Splenic lymphoma with villous lymphocytes: clinical presentation, biology and prognostic factors in a series of 100 patients. Groupe Francais d\u2019H\u00e9matologie Cellulaire (GFHC)","volume":"93","author":"Troussard","year":"1996","journal-title":"Br J Haematol."},{"key":"2020041521554117000_CIT0010","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1016\/j.humpath.2011.11.004","article-title":"CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma: a clinicopathologic study of 14 cases","volume":"43","author":"Jaso","year":"2012","journal-title":"Hum Pathol."},{"key":"2020041521554117000_CIT0011","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/modpathol.2015.42","article-title":"CD5-positive follicular lymphoma: clinicopathologic correlations and outcome in 88 cases","volume":"28","author":"Li","year":"2015","journal-title":"Mod Pathol."},{"key":"2020041521554117000_CIT0012","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/ajcp\/aqy144","article-title":"IRTA1 and MNDA expression in marginal zone lymphoma: utility in differential diagnosis and implications for classification","volume":"151","author":"Wang","year":"2019","journal-title":"Am J Clin Pathol."},{"key":"2020041521554117000_CIT0013","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.1182\/blood-2006-09-045989","article-title":"CD10-MUM1+ follicular lymphoma lacks BCL2 gene translocation and shows characteristic biologic and clinical features","volume":"109","author":"Karube","year":"2007","journal-title":"Blood."},{"key":"2020041521554117000_CIT0014","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.anndiagpath.2016.09.016","article-title":"Expression of LEF1 in mantle cell lymphoma","volume":"26","author":"O\u2019Malley","year":"2017","journal-title":"Ann Diagn Pathol."},{"key":"2020041521554117000_CIT0015","doi-asserted-by":"crossref","DOI":"10.1093\/ajcp\/138.suppl1.267","article-title":"CD5 hairy cell leukemia: a rare entity and potential diagnostic pitfall","volume":"138","author":"Sramek","year":"2012","journal-title":"Am J Clin Pathol."},{"key":"2020041521554117000_CIT0016","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.4049\/jimmunol.1002173","article-title":"A conserved enhancer element differentially regulates developmental expression of CD5 in B and T cells","volume":"185","author":"Berland","year":"2010","journal-title":"J Immunol."},{"key":"2020041521554117000_CIT0017","doi-asserted-by":"crossref","first-page":"4537","DOI":"10.1182\/blood-2002-05-1525","article-title":"Human CD5 promotes B-cell survival through stimulation of autocrine IL-10 production","volume":"100","author":"Gary-Gouy","year":"2002","journal-title":"Blood."},{"key":"2020041521554117000_CIT0018","doi-asserted-by":"crossref","first-page":"5623","DOI":"10.4049\/jimmunol.0802412","article-title":"IL-6 modulates CD5 expression in B cells from patients with lupus by regulating DNA methylation","volume":"182","author":"Garaud","year":"2009","journal-title":"J Immunol."},{"key":"2020041521554117000_CIT0019","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1182\/blood-2016-02-697185","article-title":"EBI2 overexpression in mice leads to B1 B-cell expansion and chronic lymphocytic leukemia-like B-cell malignancies","volume":"129","author":"Niss Arfelt","year":"2017","journal-title":"Blood."}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/153\/5\/646\/33049436\/aqz207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ajcp\/article-pdf\/153\/5\/646\/33049436\/aqz207.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T01:55:55Z","timestamp":1587002155000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/153\/5\/646\/5709530"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,18]]},"references-count":19,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,1,18]]},"published-print":{"date-parts":[[2020,4,15]]}},"URL":"https:\/\/doi.org\/10.1093\/ajcp\/aqz207","relation":{},"ISSN":["0002-9173","1943-7722"],"issn-type":[{"value":"0002-9173","type":"print"},{"value":"1943-7722","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5]]},"published":{"date-parts":[[2020,1,18]]}}}