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Physical activity during pregnancy reduces the risk of these APOs, yet few meet physical activity guidelines during pregnancy. Little is known about the role of sedentary behavior or sleep in APOs, a critical gap in knowledge given these behaviors comprise the majority of a 24-hour day. To address this knowledge gap, the Pregnancy 24\/7 cohort study (2020\u20132025) uses 2 devices for 24-hour activity assessment in each trimester of pregnancy to examine associations of sedentary behavior, sleep, and the 24-hour activity cycle (composition of sedentary behavior, physical activity, and sleep) with hypertensive disorders and other APOs. Participants (n\u00a0= 500) are recruited from the University of Iowa, University of Pittsburgh, and West Virginia University in early pregnancy and followed through delivery. The activPAL3 micro and Actiwatch Spectrum Plus are worn in each trimester for 7 days of 24-hour wear to assess the 24-hour activity cycle. APOs are abstracted from medical charts. This study will provide critical data to fuel future research examining how modifying the 24-hour activity cycle in pregnancy can improve maternal health.<\/jats:p>","DOI":"10.1093\/aje\/kwad208","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T18:41:08Z","timestamp":1699468868000},"page":"415-425","source":"Crossref","is-referenced-by-count":17,"title":["Study Design and Protocol of the Multisite Pregnancy 24\/7 Cohort Study"],"prefix":"10.1093","volume":"193","author":[{"given":"Kara M","family":"Whitaker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa A","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Catov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maisa","family":"Feghali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher E","family":"Kline","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Santillan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Santillan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget","family":"Zimmerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany Barone","family":"Gibbs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"issue":"1","key":"2024101708392734100_ref1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1097\/01.AOG.0000248537.89739.96","article-title":"Fetal death","volume":"109","author":"Silver","year":"2007","journal-title":"Obstet Gynecol."},{"issue":"6","key":"2024101708392734100_ref2","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1053\/j.semperi.2017.07.007","article-title":"Epidemiology of maternal morbidity and mortality","volume":"41","author":"Hirshberg","year":"2017","journal-title":"Semin Perinatol."},{"issue":"8","key":"2024101708392734100_ref3","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1161\/CIRCRESAHA.116.307547","article-title":"Cardiovascular disease in women: clinical perspectives. 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