{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:13:38Z","timestamp":1784607218239,"version":"3.55.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T00:00:00Z","timestamp":1624406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM104357"],"award-info":[{"award-number":["P20GM104357"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM121334"],"award-info":[{"award-number":["P20GM121334"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20121334"],"award-info":[{"award-number":["P20121334"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01HL051971"],"award-info":[{"award-number":["P01HL051971"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL1136684"],"award-info":[{"award-number":["R01HL1136684"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Pressure-induced constriction (PIC) is inherent to small arteries and arterioles, in which intraluminal pressure-induced vascular smooth muscle cell stretch elicits vasoconstriction. Degenerin (Deg) proteins, such as beta-epithelial Na+ channel (\u03b2ENaC), have been studied in the PIC response because they are evolutionarily linked to known mechanosensors. While loss of Deg function phenotypes are plentiful, a gain-of-function phenotype has not been studied. The aim of this study was to determine if expression of exogenous \u03b2ENaC in the isolated middle cerebral artery (MCA) enhances the PIC response.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Isolated MCA segments from female mice (24 weeks, n = 5) were transfected with enhanced green fluorescent protein\u2013\u03b2ENaC (EGFP\u2013\u03b2ENaC) or with EGFP alone, incubated overnight at 37 \u00b0C, then studied in a pressure myograph.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Mechanical\/morphological properties and vasoconstrictor responses to KCl and phenylephrine were identical in EGFP\u2013\u03b2ENaC and EGFP MCAs. In contrast, PIC responses were greater in EGFP\u2013\u03b2ENaC segments with ~2-fold greater peak myogenic tone.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These data confirm previous findings that \u03b2ENaC is critical in the PIC response. These data provide proof-of-concept that upregulating \u03b2ENaC can enhance PIC responses and lay the foundation to test the hypothesis that inflammation-mediated downregulation of \u03b2ENaC contributes to cerebrovascular dysfunction.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajh\/hpab098","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T19:13:14Z","timestamp":1624043594000},"page":"1227-1235","source":"Crossref","is-referenced-by-count":11,"title":["Expression of Exogenous Epithelial Sodium Channel Beta Subunit in the Mouse Middle Cerebral Artery Increases Pressure-Induced Constriction"],"prefix":"10.1093","volume":"34","author":[{"given":"Zoltan","family":"Nemeth","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Mississippi Medical Center , Jackson, Mississippi , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael 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