{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:15:13Z","timestamp":1777439713494,"version":"3.51.4"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T00:00:00Z","timestamp":1472169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,1]]},"abstract":"<jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>We previously reported that offspring heterozygous mice partially lacking endothelial nitric oxide synthase (eNOS) gene, and born to hypertensive eNOS\u2212\/\u2212 Knockout mother, are hypertensive. We hypothesized that those offspring when placed on high-fat diet (HFD) will undergo altered metabolic programming increasing their risk for developing metabolic syndrome.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>eNOS\u2212\/\u2212KO and wild-type mice (eNOS+\/+WT) were cross-bred to produce heterozygous offspring: maternal heterozygous (Mat, eNOS\u2212\/+), born from hypertensive eNOS\u2212\/\u2212KO mothers; and paternal heterozygous (Pat, eNOS\u2212\/+), born from normotensive WT mothers. Mat, eNOS\u2212\/+ and Pat, eNOS\u2212\/+ female were allocated to HFD or control diet (CD) until 8 weeks of age. Then a metabolic profile was obtained: weight, glucose\/insulin tolerance test (GTT, ITT), systolic blood pressure (SBP), serum fasting levels of insulin, adiponectin, leptin, and a lipid panel.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>Weight was not different between all offspring within each diet. GTT curve was higher in Mat, eNOS\u2212\/+ vs. Pat, eNOS\u2212\/+ offspring on both diet (P &amp;lt; 0.001). In ITT, glucose level at 15 minutes was higher in Mat, eNOS\u2212\/+ on HFD. Insulin level was increased in Mat, eNOS\u2212\/+ vs. Pat, eNOS\u2212\/+ on either diet. SBP was elevated in Mat, eNOS\u2212\/+ vs. Pat, eNOS\u2212\/+ on CD and was further raised in Mat, eNOS\u2212\/+ offspring on HFD (P &amp;lt; 0.001). No other differences were seen except for lower high-density lipoprotein levels in Mat, eNOS\u2212\/+ fed HFD (P &amp;lt; 0.003).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Mat, eNOS\u2212\/+ offspring exposed in utero to maternal hypertension and fed HFD postnatally have increased susceptibility for metabolic abnormalities. Thus, maternal HTN is a risk factor for altered fetal metabolic programming.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajh\/hpw088","type":"journal-article","created":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T03:18:53Z","timestamp":1472267933000},"page":"1366-1373","source":"Crossref","is-referenced-by-count":16,"title":["Adverse Effect of High-Fat Diet on Metabolic Programming in Offspring Born to a Murine Model of Maternal Hypertension"],"prefix":"10.1093","volume":"29","author":[{"given":"Monica","family":"Longo","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerrie S.","family":"Refuerzo","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lovepreet","family":"Mann","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mateo","family":"Leon","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hind N.","family":"Moussa","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baha M.","family":"Sibai","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean C.","family":"Blackwell","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology and Reproductive Sciences, McGovern Medical School at The University of Texas Health Science Center at Houston 1 , Texas , USA ."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,8,26]]},"reference":[{"key":"2024020111111935600_CIT0001","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1136\/bmj.298.6673.564","article-title":"Growth in utero, blood pressure in childhood and adult life, and mortality from cardiovascular disease","volume":"298","author":"Barker","year":"1989","journal-title":"BMJ"},{"key":"2024020111111935600_CIT0002","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0140-6736(89)90710-1","article-title":"Weight in infancy and death from ischaemic heart disease","volume":"2","author":"Barker","year":"1989","journal-title":"Lancet"},{"key":"2024020111111935600_CIT0003","doi-asserted-by":"crossref","first-page":"592408","DOI":"10.1155\/2011\/592408","article-title":"Maternal obesity and developmental programming of metabolic disorders in offspring: evidence from animal models","volume":"2011","author":"Li","year":"2011","journal-title":"Exp Diabetes Res"},{"key":"2024020111111935600_CIT0004","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1113\/jphysiol.2011.223305","article-title":"Nature, nurture or nutrition? 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