{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T20:07:28Z","timestamp":1770840448273,"version":"3.50.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Russian Scientific Foundation","award":["18-15-00222"],"award-info":[{"award-number":["18-15-00222"]}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["18-15-00222"],"award-info":[{"award-number":["18-15-00222"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["18-15-00222"],"award-info":[{"award-number":["18-15-00222"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Previous studies implicated cardiotonic steroids, including Na\/K-ATPase inhibitor marinobufagenin (MBG), in the pathogenesis of preeclampsia (PE). We demonstrated that MBG induces fibrosis via mechanism involving inhibition of Fli1, a nuclear transcription factor and a negative regulator of collagen-1 synthesis. We hypothesized that PE blockade of increased MBG with antibody would lessen the fibrosis of umbilical arteries and lower the blood pressure in rats with PE.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>We tested 36 pregnant Sprague-Dawley rats in which 12 were made hypertensive by 1.8% Na supplementation (days 6\u201319 of gestation), 12 pregnant rats served controls. At day 19, PE rats received one intraperitoneal injection of polyclonal anti-MBG-4 antibody (0.5 ug\/ml) for 4 hours.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>PE was associated with higher blood pressure (117 \u00b1 2 vs. 107 \u00b1 2 mm Hg; P &amp;lt; 0.01), plasma MBG levels (1.54 \u00b1 0.34 vs. 0.49 \u00b1 0.11 nmol\/L; P &amp;lt; 0.01), protein excretion (26 vs. 12 mg\/24 hours), sFlt-1 (3-fold), decrease in Fli1 (7-fold) and increase in collagen-1 in aorta (4-fold) vs. control rats (all P &amp;lt; 0.01). In 12 rats treated with polyclonal anti-MBG-4 antibody blood pressure dropped (93 \u00b1 3 mm Hg) and Fli1 was decreased much less (2-fold; P &amp;lt; 0.01 vs. nontreated rats).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>These results demonstrate that in experimental PE elevated MBG level is implicated in umbilical fibrosis via suppression of Fli1.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ajh\/hpz180","type":"journal-article","created":{"date-parts":[[2019,11,9]],"date-time":"2019-11-09T12:10:31Z","timestamp":1573301431000},"page":"514-519","source":"Crossref","is-referenced-by-count":6,"title":["Antibody against Na\/K-ATPase Inhibitor Lowers Blood Pressure and Increases Vascular Fli1 in Experimental Preeclampsia"],"prefix":"10.1093","volume":"33","author":[{"given":"Natalia I","family":"Agalakova","sequence":"first","affiliation":[{"name":"Sechenov Institute of Evolutionary Physiology and Biochemistry , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitaly A","family":"Reznik","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, St. Petersburg State Pediatric Medical University , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga V","family":"Nadei","sequence":"additional","affiliation":[{"name":"Sechenov Institute of Evolutionary Physiology and Biochemistry , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan A","family":"Ershov","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, St. Petersburg State Pediatric Medical University , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga S","family":"Rassokha","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, St. Petersburg State Pediatric Medical University , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina L","family":"Vasyutina","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, Almazov National Medical Research Centre , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry O","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, St. Petersburg State Pediatric Medical University , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C David","family":"Adair","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Tennessee , Chattanooga, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael M","family":"Galagudza","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, Almazov National Medical Research Centre , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexei Y","family":"Bagrov","sequence":"additional","affiliation":[{"name":"Sechenov Institute of Evolutionary Physiology and Biochemistry , St. Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,12]]},"reference":[{"key":"2022100608484396000_CIT0001","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1097\/01.ede.0000152912.02042.cd","article-title":"Long-term mortality after 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