{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:14:47Z","timestamp":1789139687788,"version":"build-2803163510"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Pseudomonas aeruginosa (PA) associates with poor clinical outcomes, however, exhibits inter-individual clinical and treatment heterogeneity in bronchiectasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sputum metagenomes from n\u2009=\u2009600 individuals with bronchiectasis from 10 countries were assessed to derive PA subgroups based on abundance (abundotypes) and\/or microbial interactions (interactotypes). Longitudinal dynamics of these subgroups were assessed in two common clinical scenarios: acute exacerbation and PA eradication. Transition probabilities for each subgroup were estimated in these scenarios.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Four PA-abundotypes with comparable exacerbation risk were identified: PA-dominant (PD), Moderate PA-dominant (MPD), Other Microbe Dominant (OMD) and High Diversity (HD). Four separate PA-interactotypes were characterized: BC1 (PA-Neisseria interactions); BC2 (PA-S. aureus &amp; E. coli interactions); BC3 (PA-Streptococcus interactions) and BC4 (mixed interactions). Core (conserved) interactions with commensal taxa were shared across all interactotypes while ancillary (variable) interactions remain interactotype-specific. Abundotypes correlate with bronchiectasis severity, symptoms and lung function while interactotypes stratify exacerbation risk with BC3 the lowest-risk group. BC1 and BC4 exhibit 1.45- and 1.44-higher fold rates (p\u2009=\u20090.037 and p\u2009=\u20090.048 respectively) for exacerbation while BC2 demonstrates a non-significant increase in exacerbation risk (1.32-fold; p\u2009=\u20090.18). In hypothesis-generating analyses using a small longitudinal cohort, PA-abundotypes remain stable through exacerbations (n\u2009=\u20096) while PA-interactotypes shift substantially suggesting dynamic responsiveness to acute clinical states. During PA eradication (n\u2009=\u200911), abundotypes exhibit structured transition with standard therapy while interactotypes demonstrate no consistent patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PA-abundotypes and interactotypes represent independent traits capturing different dimensions of PA ecology and potentially contributing to observed clinical and treatment heterogeneity in bronchiectasis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ajrccm\/aamag464","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:50:12Z","timestamp":1789041012000},"source":"Crossref","is-referenced-by-count":0,"title":["Pseudomonas Aeruginosa Abundotypes and Interactotypes Reflect Clinical Heterogeneity in Bronchiectasis"],"prefix":"10.1093","author":[{"given":"Jayanth Kumar","family":"Narayana","sequence":"first","affiliation":[{"name":"Nanyang Technological University Lee Kong Chian School of Medicine, , Singapore,","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tavleen Kaur","family":"Jaggi","sequence":"additional","affiliation":[{"name":"Nanyang Technological University Lee Kong Chian School of Medicine, , Singapore,","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Dimakou","sequence":"additional","affiliation":[{"name":"Attica\u2019\u2019 Rehabilitation Center \u201c , Athens,","place":["Greece"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fransiskus Xaverius","family":"Ivan","sequence":"additional","affiliation":[{"name":"Nanyang Technological University Lee Kong Chian School of Medicine, , Singapore,","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miche\u00e1l","family":"Mac Aog\u00e1in","sequence":"additional","affiliation":[{"name":"St. James\u2019s Hospital Department of Biochemistry, , Dublin,","place":["Ireland"]},{"name":"Trinity College Clinical Biochemistry Unit, School of Medicine, , Dublin,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mau Ern","family":"Poh","sequence":"additional","affiliation":[{"name":"Universiti Malaya Department of Medicine, Faculty of Medicine, , Kuala Lumpur,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca C","family":"Hull","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandani","family":"Hennayake","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merete B","family":"Long","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma D","family":"Johnson","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly","family":"Lind","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter C","family":"Goeminne","sequence":"additional","affiliation":[{"name":"AZ Nikolaas Department of Respiratory Disease, ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Shteinberg","sequence":"additional","affiliation":[{"name":"Pulmonology Institute and CF Center, Carmel Medical Center , Haifa,","place":["Israel"]},{"name":"The Technion- Israel Institute of Technology , B. Rappaport Faculty of Medicine, Haifa,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"De Soyza","sequence":"additional","affiliation":[{"name":"Population and Health Science Institute, Newcastle University and NIHR Biomedical Research Centre for Ageing, Freeman Hospital , Newcastle,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Aliberti","sequence":"additional","affiliation":[{"name":"Humanitas University Department of Biomedical Sciences, , Via Rita Levi Montalcini 4, 20072, Pieve Emanuele, Milan,","place":["Italy"]},{"name":"IRCCS Humanitas Research Hospital, Respiratory Unit , Via Manzoni 56, 20089, Rozzano, Milan,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josje","family":"Altenburg","sequence":"additional","affiliation":[{"name":"Amsterdam Medical Center Department of Pulmonary Diseases, , Amsterdam, The","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles S","family":"Haworth","sequence":"additional","affiliation":[{"name":"Royal Papworth Hospital and University of Cambridge Cambridge Centre for Lung Infection, , Cambridge,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriol","family":"Sibila","sequence":"additional","affiliation":[{"name":"University of Barcelona Servicio de Neumolog\u00eda, Instituto Cl\u00ednico de Respiratorio. IDIBAPS, CIBERES. Hospital Cl\u00ednic, , Barcelona,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Polverino","sequence":"additional","affiliation":[{"name":"Hospital Universitari Vall d\u2019Hebron, Vall d\u2019Hebron Institut de Recerca (VHIR), Vall d\u2019Hebron Barcelona Hospital Campus, CIBERES Pneumology Department, , Barcelona,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R","family":"Loebinger","sequence":"additional","affiliation":[{"name":"Royal Brompton and Harefield Hospitals and National Heart and Lung Institute, Imperial College London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Shoemark","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]},{"name":"Royal Brompton and Harefield Hospitals and National Heart and Lung Institute, Imperial College London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix C","family":"Ringshausen","sequence":"additional","affiliation":[{"name":"Hannover Medical School, and German Center for Lung Research (DZL) Department of Respiratory Medicine and Infectious Diseases, , Hannover,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Lorent","sequence":"additional","affiliation":[{"name":"University Hospitals Leuven Department of Respiratory Diseases, , Leuven,","place":["Belgium"]},{"name":"KU Leuven Department of Chronic Diseases and Metabolism, Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), , Leuven,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Blasi","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Milano Department of Pathophysiology and Transplantation, ,","place":["Italy"]},{"name":"Respiratory and Cystic Fibrosis Unit, Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico Milano ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D","family":"Chalmers","sequence":"additional","affiliation":[{"name":"University of Dundee, Ninewells Hospital and Medical School Division of Respiratory Medicine and Gastroenterology, , Dundee,","place":["UK"]},{"name":"University of Oxford John Radcliffe Department of Medicine, , Oxford,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay H","family":"Chotirmall","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore,","place":["Singapore"]},{"name":"Tan Tock Seng Hospital Department of Respiratory and Critical Care Medicine, , Singapore,","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,11]]},"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/advance-article-pdf\/doi\/10.1093\/ajrccm\/aamag464\/71032675\/aamag464.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/advance-article-pdf\/doi\/10.1093\/ajrccm\/aamag464\/71032675\/aamag464.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:42:58Z","timestamp":1789137778000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/advance-article\/doi\/10.1093\/ajrccm\/aamag464\/8790593"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/ajrccm\/aamag464","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]},"article-number":"aamag464"}}