{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:59:51Z","timestamp":1789523991732,"version":"build-2803163510"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States National Science Foundation Division of Environmental Biology","award":["1119944"],"award-info":[{"award-number":["1119944"]}]},{"name":"Chilean Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica-Fondo Nacional de Desarrollo Cientifico y Technol\u00f3gico","award":["1120619"],"award-info":[{"award-number":["1120619"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background and Aims<\/jats:title>\n                  <jats:p>Canyon stream beds in the hyperarid Atacama Desert surprisingly harbour magnificent groves of endemic giant horsetail wetland plants, Equisetum xylochaetum. Our previous metagenomic study of eukaryotes closely associated with this plant indicated that the microbiome included prokaryotes that might likewise influence host success and environment. We explored this possibility by using the metagenomic sequence to characterize prokaryote taxa and functional genes present in the microbiome of E. xylochaetum sampled from remote sites differing in the degree of anthropogenic disturbance. We focused on biogeochemical functions known to be important in wetland ecosystems.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>To ensure that analyses were conducted on microbes most closely associated with plants, we extracted DNA from well-washed plant organs whose microbial biofilms were revealed with scanning electron microscopy. To assess the benefits of longer sequences for taxonomic and gene classifications, results of analyses performed using contigs were compared with those obtained with unassembled reads. We employed methods widely used to estimate genomic coverage of single taxa for genomic analysis to infer relative abundances of taxa and functional genes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key Results<\/jats:title>\n                  <jats:p>Key functional bacterial genera (e.g. Hydrogenophaga, Sulfuritalea and Rhodoferax) inferred from taxonomic and functional gene analysis of contigs \u2013 but not unassembled reads \u2013 to occur on surfaces of (or within) plants at relatively high abundance (&amp;gt;50\u00d7 genomic coverage) indicated roles in nitrogen, sulfur and other mineral cycling processes. Comparison between sites revealed impacts on biogeochemical functions, e.g. reduced levels of the nifH gene marker under disturbance. Vanadium nitrogenases were more important than molybdenum nitrogenases, indicated by both functional genes and taxa such as Rhodomicrobium and Phaeospirillum inferred from contigs but not unassembled reads.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our contig-based metagenomic analyses revealed that microbes performing key wetland biogeochemical functions occur as tightly adherent biofilms on the plant body, not just in water or sediments, and that disturbance reduces such functions, providing arguments for conservation efforts.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/aob\/mcac060","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T20:07:36Z","timestamp":1651781256000},"page":"65-75","source":"Crossref","is-referenced-by-count":5,"title":["Nitrogen fixation and other biogeochemically important features of Atacama Desert giant horsetail plant microbiomes inferred from metagenomic contig analysis"],"prefix":"10.1093","volume":"130","author":[{"given":"Anchittha","family":"Satjarak","sequence":"first","affiliation":[{"name":"Plants of Thailand Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University , Bangkok , Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda E","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Wisconsin-Madison , Madison, WI , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie T","family":"Trest","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Wisconsin-Madison , Madison, WI , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"Zedler","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Wisconsin-Madison , Madison, WI , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer J","family":"Knack","sequence":"additional","affiliation":[{"name":"Department of Botany, University of Wisconsin-Madison , Madison, WI , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Arancibia-Avila","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, University of B\u00edo-B\u00edo , Chillan , Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"2022071916384305600_CIT0001","first-page":"47","article-title":"The capacity of northern peatlands for long-term carbon sequestration","volume-title":"Biogeosciences","author":"Alexandrov","year":"2020"},{"key":"2022071916384305600_CIT0002","doi-asserted-by":"crossref","first-page":"55","DOI":"10.2307\/2399466","article-title":"Effects of aridity on plant diversity in the northern Chilean Andes: results of a 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