{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:01:44Z","timestamp":1787238104293,"version":"build-2736575974"},"reference-count":121,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Charles University Research Centre programme","award":["PRIMUS\/19\/SCI\/02"],"award-info":[{"award-number":["PRIMUS\/19\/SCI\/02"]}]},{"DOI":"10.13039\/501100004240","name":"Czech Academy of Sciences","doi-asserted-by":"publisher","award":["RVO 67985939"],"award-info":[{"award-number":["RVO 67985939"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Operational Program Integrated Infrastructure"},{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","award":["ITMS313011T721"],"award-info":[{"award-number":["ITMS313011T721"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"e-INFRA CZ project","award":["ID:90254"],"award-info":[{"award-number":["ID:90254"]}]},{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youth and Sports of the Czech Republic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Plants have colonized diverse habitats, with their ecological success depending on key adaptive traits. Among these, reproductive characteristics such as pollen and ovule production are critical. The allocation to sexual reproduction is likely shaped by diverse selective pressures and constraints. This study investigates the evolution of pollen and ovule production and its drivers in the Brassicaceae family.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analysed 158 Brassicaceae species, recorded pollen and ovule numbers, and used phylogenetic analyses to characterize the evolution of these traits. We then cultivated a subset of 66 species in a common garden and tested the association of pollen and ovule production with environmental conditions of original habitats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results and Conclusions<\/jats:title>\n                    <jats:p>Ovule production is strongly constrained by phylogeny, whereas pollen production is more evolutionarily flexible. Expectedly, recurrent mating system shifts played a role in this observation, as selfing species consistently produced fewer pollen grains but did not show significant changes in ovule number, supporting selection for reduced male investment in response to increased mating efficiency. In contrast, environmental associations with both traits were limited yet significant, with pollen and ovule production evolving according to different environmental pressures. Polyploidy did not influence the evolution of these traits. These phylogeny-informed findings refine our understanding of the evolutionary drivers of sexual allocation, demonstrating that male and female investment evolve under distinct selective pressures in hermaphroditic species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/aob\/mcaf176","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T11:55:02Z","timestamp":1754049302000},"page":"868-882","source":"Crossref","is-referenced-by-count":1,"title":["Divergent evolutionary trajectories of male and female investment in the Brassicaceae"],"prefix":"10.1093","volume":"138","author":[{"given":"Mohammadjavad","family":"Haghighatnia","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Science, Charles University , Prague CZ-128 01 ,","place":["Czech Republic"]},{"name":"Institute of Botany, The Czech Academy of Sciences , Pr\u016fhonice 252 43 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2710-8102","authenticated-orcid":false,"given":"Marek","family":"Svitok","sequence":"additional","affiliation":[{"name":"Department of Biology and General Ecology, Faculty of Ecology and Environmental Sciences, Technical University in Zvolen , Zvolen 960 01 ,","place":["Slovakia"]},{"name":"Department of Forest Ecology, Faculty of Forestry and Wood Sciences, University of Life Sciences Prague , Praha 6, Suchdol 165 21 ,","place":["Czech Republic"]},{"name":"Plant Science and Biodiversity Centre, Slovak Academy of Sciences , Bratislava 845 23 ,","place":["Slovakia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus A","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Plant Systematics, Centre for Organismal Studies, Heidelberg University , Heidelberg 69120 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roswitha","family":"Schmickl","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Charles University , Prague CZ-128 01 ,","place":["Czech Republic"]},{"name":"Institute of Botany, The Czech Academy of Sciences , Pr\u016fhonice 252 43 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-8104","authenticated-orcid":false,"given":"Cl\u00e9ment","family":"Lafon Placette","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Charles University , Prague CZ-128 01 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,4]]},"reference":[{"key":"2026081905372626100_mcaf176-B1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1093\/aob\/mcaf055","article-title":"The 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