{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T22:51:02Z","timestamp":1780354262526,"version":"3.54.1"},"reference-count":71,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012088","name":"Arcadia Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001047","name":"Ian Potter Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001047","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Friends and Foundation of the Botanic Gardens"},{"name":"Maxwell Foundation"},{"name":"Greatorex Foundation"},{"name":"HSBC Bank"},{"name":"Bloomberg"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Recent studies on desiccation-tolerant Australian rainforest seeds demonstrated that some were short-lived in storage. We sought to understand structural changes of storage lipids that might occur during storage at \u221220 \u00b0C that could contribute to a short lifespan.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used differential scanning calorimetry (DSC) to examine exothermic and endothermic transitions during freezing and thawing in dry seed samples of 23 species. Seed samples and extracted triacylglycerols (TAGs) were cooled to \u2212150 \u00b0C and rewarmed to 50 \u00b0C at 10 \u00b0C min\u22121; slower and faster rates of cooling\/warming were used for a subset of species to examine lipid crystallization and melting kinetics. Thermograms were analysed for temperature and enthalpy of observed peaks, and these were compared with expected values to detect anomalies. Extracted lipids were further analysed using gas chromatography to characterize fatty acid composition. The thermal profiles of six species were used to design experiments comparing the impact of storage at \u221220 \u00b0C with storage at temperatures outside the range of thermal transitions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Thermal activity was detected in 22 species within the narrow temperature range of \u221230 and \u221210 \u00b0C; activity at broader temperature ranges was also detected depending on species, cooling protocol and fatty acid composition. A profound interaction between DSC parameters and time at low temperature, as well as fatty acid composition, suggested that TAG crystallization rates contribute to low-temperature sensitivity. We confirmed that damage from TAG crystallization could be avoided by storing seeds at temperatures above TAG crystallization and melting events; storage at cryogenic temperatures improved survival over storage at \u221220 \u00b0C but requires further optimization to maintain pre-storage germination potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We conclude that the crystallization and melting of TAGs during storage may negatively impact seed longevity. Seed thermal profiles and rate of TAG crystallization may serve as predictive tools for sensitivity to storage at \u221220 \u00b0C.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/aob\/mcaf181","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T11:36:39Z","timestamp":1754393799000},"page":"1547-1564","source":"Crossref","is-referenced-by-count":2,"title":["Thermal behaviour of lipids in short-lived seeds of Australian rainforest species"],"prefix":"10.1093","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9843-6616","authenticated-orcid":false,"given":"Karen D","family":"Sommerville","sequence":"first","affiliation":[{"name":"Australian PlantBank, Botanic Gardens of Sydney , Mount Annan, NSW 2567","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Hill","sequence":"additional","affiliation":[{"name":"National Laboratory for Genetic Resources Preservation, United States Department of Agriculture , Fort Collins, CO 80521 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A","family":"Offord","sequence":"additional","affiliation":[{"name":"Australian PlantBank, Botanic Gardens of Sydney , Mount Annan, NSW 2567","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Walters","sequence":"additional","affiliation":[{"name":"National Laboratory for Genetic Resources Preservation, United States Department of Agriculture , Fort Collins, CO 80521 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"2025122013520877500_mcaf181-B1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1079\/SSR2003135","article-title":"Acquisition of desiccation tolerance in developing oil palm (Elaeis guineensis Jacq.) embryos in planta and in vitro in relation to sugar 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