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Here, we show that the rice PLETHORA transcription factor OsPLT1 suppresses rice tolerance to P-deficient conditions. Under P-starved conditions, osplt1 mutants accumulated more biomass and exhibited higher soluble and total P concentrations, whereas OsPLT1-overexpressing lines were hypersensitive to P deficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>Mechanistically, osplt1 mutants displayed reduced P retention in root cell walls, particularly in the water-soluble pectin fraction, accompanied by increased water-soluble pectin uronic acid content and elevated pectin methylesterase activity. Enzymatic digestion assays confirmed enhanced release of wall-bound P in the mutants. Moreover, osplt1 mutants showed higher nitric oxide (NO) accumulation under P deficiency, whereas sodium nitroprusside treatment largely reduced the differences in cell wall P and soluble P between Nip and osplt1 mutants, suggesting that NO is associated with OsPLT1-dependent P reutilization. The phosphate uptake-related gene, OsPT6, was also more strongly induced in the mutants, which may contribute to improved P acquisition. Notably, OsPHR1\/2 and representative PHR-responsive genes showed comparable expression among genotypes under the same P condition, suggesting that OsPLT1-mediated regulation may not primarily depend on major changes in canonical PHR signalling activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Together, these results reveal a link between root developmental control and P homeostasis and suggest that OsPLT1 may provide a useful genetic target for enhancing P-use efficiency in rice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/aob\/mcag142","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T09:54:06Z","timestamp":1779357246000},"page":"1343-1356","source":"Crossref","is-referenced-by-count":1,"title":["<i>OsPLT1<\/i>\n                    negatively regulates phosphorus deficiency tolerance by modulating nitric oxide-mediated cell wall phosphorus reutilization in rice"],"prefix":"10.1093","volume":"138","author":[{"given":"Yong Qiang","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]},{"name":"School of Environment Science and Spatial Informatics, China University of Mining and Technology , Xuzhou 221116 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong Wei","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]},{"name":"School of Environment Science and Spatial Informatics, China University of Mining and Technology , Xuzhou 221116 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia Qi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiu","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Environment Science and Spatial Informatics, China University of Mining and Technology , Xuzhou 221116 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6474-8198","authenticated-orcid":false,"given":"Ren Fang","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2052-2212","authenticated-orcid":false,"given":"Xiao Fang","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008 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