{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:14:25Z","timestamp":1781662465724,"version":"3.54.5"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Chinese Academy of Medical Sciences Oxford Institute"},{"name":"Innovation Fund for Medical Sciences","award":["2021-1-I2M-052"],"award-info":[{"award-number":["2021-1-I2M-052"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Fat transplantation is a common method employed to treat soft-tissue defects. The dedifferentiation of mature adipocytes has been well documented, but whether it occurs after fat transplantation remains unclear.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The major purpose of this project was to investigate the dedifferentiation of mature adipocytes after fat transplantation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Human lipoaspirate tissue was obtained from 6 female patients who underwent esthetic liposuction. Mature adipocytes were extracted and labeled with PKH26, mixed with lipoaspirate, and injected into nude mice. In addition, PKH26+ adipocytes were subjected to a ceiling culture. Grafted fat was harvested from nude mice, and stromal vascular fragment cells were isolated. The immunophenotype of PKH26+ cells was detected by flow cytometry analysis at 2 days and 1 week. The PKH26+ cells were sorted and counted at 2 and 4 weeks to verify their proliferation and multilineage differentiation abilities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Two days after transplantation, almost no PKH26+ cells were found in the stromal vascular fragment cells. The PKH26+ cells found 1 week after transplantation showed a positive expression of cluster of differentiation (CD) 90 (CD90) and CD105 and a negative expression of CD45. This indicates that the labeled adipocytes were dedifferentiated. Its pluripotency was further demonstrated by fluorescent cell sorting and differentiation culture in vitro. In addition, the number of live PKH26+ cells at week 4 [(6.83 \u00b1 1.67) \u00d7 104] was similar with that at week 2 [(7.11 \u00b1 1.82) \u00d7 104].<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Human mature adipocytes can dedifferentiate into stem cell-like cells in vivo after fat transplantation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/asj\/sjab402","type":"journal-article","created":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T14:10:44Z","timestamp":1642255844000},"page":"NP423-NP431","source":"Crossref","is-referenced-by-count":17,"title":["Dedifferentiation of Human Adipocytes After Fat Transplantation"],"prefix":"10.1093","volume":"42","author":[{"given":"Yimeng","family":"Chai","sequence":"first","affiliation":[{"name":"Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences , Beijing , People\u2019s Republic of 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