{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:02:12Z","timestamp":1792069332643,"version":"4.4.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101839"],"award-info":[{"award-number":["82101839"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Skin T cells are essential for maintaining the skin's immune barrier and promoting early wound healing. Exosomes from adipose-derived stem cells (ADSCs-exo) can accelerate wound healing and reduce inflammation, but their impact on skin T-cell inflammation is unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aims to explore ADSCs-exo\u2019s regulatory effects on skin T cells and wound inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>ADSCs-exo were isolated by differential ultracentrifugation. An in vitro inflammation model using the human skin T-cell line HuT 78 was established to analyze the effects of ADSCs-exo on T-cell activation markers, inflammatory cytokines, and PI3K\/Akt signaling. Apoptosis in HuT 78 cells was assessed with Calcein-AM\/PI staining. A full-thickness skin injury model in C57 mice was used to evaluate ADSCs-exo's impact on dendritic epidermal T cells (DETCs) and inflammatory cytokine expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Phorbol 12-myristate 13-acetate (PMA) enhanced interleukin (IL)-2, IL-17A, tumor necrosis factor alpha, and interferon gamma mRNA expression elevated T cell activation marker CD25 and reduced Akt\/PI3K phosphorylation in HuT 78 cells, while inducing apoptosis. Although ADSCs-exo alone showed no CD25 modulation, their co-administration with PMA attenuated CD25 expression, inhibited IL-2 and IL-17A, and enhanced Akt\/PI3K phosphorylation compared with PMA alone. Furthermore, ADSCs-exo can reverse the proapoptotic effect of PMA. In vivo, DETCs comprised 1% of mouse epidermal cells and increased at the wound margins postinjury. ADSCs-exo reduced both DETC recruitment and IL-17A levels during early wound healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ADSCs-exo inhibited PMA-induced skin T-cell activation and inflammatory cytokine expression. Although acute trauma increased DETC expression at the wound site, ADSCs-exo inhibited early DETC and IL-17A expression, preventing excessive inflammation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/asj\/sjaf040","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T08:18:44Z","timestamp":1742199524000},"page":"723-734","source":"Crossref","is-referenced-by-count":10,"title":["Exosomes From Adipose-Derived Stem Cells Inhibit Skin T-Cell Activation and Alleviate Wound 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