{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:26:23Z","timestamp":1787012783122,"version":"build-2736575974"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The SPY Fluorescence Imaging system enables intraoperative visualization of microvascular perfusion. Recently, SPY has been integrated with Quantitative Perfusion (QP) software to provide numerical assessment of relative tissue perfusion; however, clinically actionable perfusion thresholds predictive of mastectomy skin necrosis complications have not been clearly established.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The aim of this study was to evaluate the predictive performance of proposed SPY Portable Handheld Imaging Quantitative Perfusion (PHI QP) perfusion thresholds in patients undergoing staged breast reconstruction and to explore a QP-specific cutoff while accounting for nitroglycerin ointment use as a potential confounder.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Patients undergoing staged breast reconstruction with tissue expander placement were included. SPY-PHI QP was used intraoperatively to assess relative perfusion. Diagnostic performance metrics\u2014including sensitivity, specificity, positive predictive value, negative predictive value, accuracy, and odds ratios\u2014were calculated. A subanalysis excluding patients who received nitroglycerin ointment was performed to identify a QP-specific cutoff in unmanipulated tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fifty-eight patients (83 breasts) were included. Using a 33% perfusion threshold, SPY-PHI QP demonstrated high sensitivity (87.5%) and negative predictive value (98.3%) for mastectomy skin flap necrosis. After adjusting for nitroglycerin use, the association between low perfusion and mastectomy skin flap necrosis was attenuated. In patients who did not receive nitroglycerin, a perfusion threshold of 30% demonstrated optimal diagnostic performance (sensitivity 100%, specificity 76.7%, area under the curve 0.8875).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SPY-PHI QP provides quantitative, real-time assessment of tissue perfusion during breast reconstruction. In patients not treated with nitroglycerin, a 30% perfusion threshold may be predictive of wound complications. These findings support further investigation of QP-specific thresholds in larger, prospective studies that account for adjunctive interventions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Level of Evidence: 4 (Therapeutic)<\/jats:title>\n                    <jats:p>For image description, please refer to the figure legend and surrounding text.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/asj\/sjag099","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:39:38Z","timestamp":1779190778000},"page":"NP81-NP87","source":"Crossref","is-referenced-by-count":0,"title":["Establishing a Predictive Perfusion Threshold Using SPY-PHI QP in Staged Breast Reconstruction: A Prospective Evaluation With Nitroglycerin Adjustment"],"prefix":"10.1093","volume":"46","author":[{"given":"Nikki","family":"Rezania","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly A","family":"Harmon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Okensama","family":"La-Anyane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3752-847X","authenticated-orcid":false,"given":"Annie","family":"Fritsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7117-0394","authenticated-orcid":false,"given":"Helen","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Seu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quyen","family":"Diep","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Kokosis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"2026081719565786500_sjag099-B1","doi-asserted-by":"publisher","first-page":"21S","DOI":"10.1097\/PRS.0000000000005947","article-title":"The importance of tissue perfusion in reconstructive breast surgery","volume":"144","author":"Frey","year":"2019","journal-title":"Plast Reconstr Surg"},{"key":"2026081719565786500_sjag099-B2","doi-asserted-by":"publisher","first-page":"1454","DOI":"10.1007\/s00266-020-01784-1","article-title":"Fat necrosis after DIEP flap breast reconstruction: a review of perfusion-related causes","volume":"44","author":"Bhullar","year":"2020","journal-title":"Aesthetic Plast Surg"},{"key":"2026081719565786500_sjag099-B3","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/s0750-7658(87)80357-x","article-title":"Absorption of CO2: principles and use","volume":"6","author":"Benhamou","year":"1987","journal-title":"Ann Fr Anesth Reanim"},{"key":"2026081719565786500_sjag099-B4","doi-asserted-by":"publisher","first-page":"448e","DOI":"10.1097\/PRS.0000000000000025","article-title":"Whom should we SPY? A cost analysis of laser-assisted indocyanine green angiography in prevention of mastectomy skin flap necrosis during prosthesis-based breast reconstruction","volume":"133","author":"Kanuri","year":"2014","journal-title":"Plast Reconstr Surg"},{"key":"2026081719565786500_sjag099-B5","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.breast.2010.06.006","article-title":"Factors affecting post-operative complications following skin sparing mastectomy with immediate breast reconstruction","volume":"20","author":"Davies","year":"2011","journal-title":"Breast"},{"key":"2026081719565786500_sjag099-B6","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1002\/bjs.5815","article-title":"Late results of skin-sparing mastectomy followed by immediate breast reconstruction","volume":"94","author":"Meretoja","year":"2007","journal-title":"Br J 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